| Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2021: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2020: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
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| Outline of Final Research Achievements |
Intercellular communication between cancer cells and stromal cells is essential for drug resistance and metastasis formation of cancer, exosomes play a important role as messenger. In our study of drug resistance, exosomes secreted from sunitinib-resistant tumor have shown distinct miRNA profiles compared with control. Further study is required to elucidate the mechanism by which exosomes induce drug resistance. In our study of metastasis formation, we established a bone metastatic RCC cell line. Treatment of mice with exosomes collected from culture supernatant of bone metastatic RCC cells promoted angiogenesis in bone in a time-dependent manner and increased bone metastasis. We showed that exosomal CD13 contributed to angiogenesis. In addition, exosomes from bone metastatic RCC contained abundant CD13 and promoted angiogenesis compared with exosomes from RCC without metastasis. Our findings provide a new insight to the role of exosome in cancer metastasis and drug resistance.
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