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Estimation of the environmental pollution with antimicrobial-resistant bacteria by human activities using genomic and mathematics analyses

Research Project

Project/Area Number 18H04073
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 58:Society medicine, nursing, and related fields
Research InstitutionGifu University

Principal Investigator

Asai Tetsuo  岐阜大学, 大学院連合獣医学研究科, 教授 (10509764)

Co-Investigator(Kenkyū-buntansha) 安藤 匡子  鹿児島大学, 農水産獣医学域獣医学系, 准教授 (10466914)
源 利文  神戸大学, 人間発達環境学研究科, 教授 (50450656)
森部 絢嗣  岐阜大学, 社会システム経営学環, 准教授 (50456620)
大松 勉  東京農工大学, 農学部, 准教授 (60455392)
臼井 優  酪農学園大学, 獣医学群, 准教授 (60639540)
鈴木 正嗣  岐阜大学, 応用生物科学部, 教授 (90216440)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥43,420,000 (Direct Cost: ¥33,400,000、Indirect Cost: ¥10,020,000)
Fiscal Year 2021: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2020: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2019: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2018: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Keywords薬剤耐性 / 野生動物 / 環境DNA解析 / 地理情報解析 / セファロスポリン耐性 / キノロン耐性 / 環境 / 環境DNA分析 / 数理解析 / 人間活動 / 大腸菌 / GIS解析 / 環境DNA
Outline of Final Research Achievements

The purpose of this study is to elucidate the mode of transmission of antimicrobial-resistant bacteria released from human activities, control the spread of antimicrobial-resistant bacteria in the environment, and reduce the effects of environment-derived antimicrobial-resistant bacteria on humans. The prevalence of antimicrobial resistance in 963 wildlife-derived stocked strains from 2013 to 2017 and 894 wildlife-derived strains collected from 2018 to 2021 was low, and no change in proportion was observed. Next, we analyzed the geographic information of the captured sites of wild animals carrying antimicrobial-resistant bacteria, suggesting their relevance to human social activities. And animals of origin of antimicrobial-resistant bacteria were estimated by environmental DNA analysis in environmental water, suggesting that they may be derived from livestock.

Academic Significance and Societal Importance of the Research Achievements

人医療や動物医療分野での薬剤耐性モニタリングが実施されてきたが、国内の野生動物を対象に、統一した手法で薬剤耐性の発現状況を提供し、今後のモニタリングの方向性を示した。また、古典的な微生物学的手法(薬剤耐性菌の分離)と生態情報を利用した数理解析および環境DNA解析を融合することで自然環境における薬剤耐性菌の伝播・拡散様式の一端を明らかにした。本研究により、人間活動からの薬剤耐性菌の環境中での拡散を制御する必要があることを示唆した。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (23 results)

All 2022 2021 2020 2019 2018

All Journal Article (10 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 9 results,  Open Access: 6 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Journal Article] Antimicrobial resistance in Enterobacteriaceae isolated from arthropods in Gifu City, Japan2021

    • Author(s)
      Odoi Justice O.、Yamamoto Miku、Sugiyama Michiyo、Asai Tetsuo
    • Journal Title

      Microbiology and Immunology

      Volume: 65 Issue: 3 Pages: 136-141

    • DOI

      10.1111/1348-0421.12878

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Antimicrobial-Resistant Escherichia coli in Migratory Greater White-Fronted Geese (Anser albifrons) and their Habitat in Miyajimanuma, Japan2021

    • Author(s)
      Fukuda Akira、Usui Masaru、Ushiyama Katsumi、Shrestha Dipti、Hashimoto Nagisa、Sakata Masayuki K.、Minamoto Toshifumi、Yoshida Osamu、Murakami Kanako、Tamura Yutaka、Asai Tetsuo
    • Journal Title

      Journal of Wildlife Diseases

      Volume: 57 Issue: 4 Pages: 954-958

    • DOI

      10.7589/jwd-d-21-00006

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of antimicrobial resistance in bacteria isolated from Great Cormorants (<i>Phalacrocorax carbo hanedae</i>) in Japan2021

    • Author(s)
      ODOI Justice Opare、SUGIYAMA Michiyo、KITAMURA Yuko、SUDO Akiko、OMATSU Tsutomu、ASAI Tetsuo
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 83 Issue: 8 Pages: 1191-1195

    • DOI

      10.1292/jvms.21-0108

    • NAID

      130008072094

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Association between the blaCTX-M-14-harboring Escherichia coli Isolated from Weasels and Domestic Animals Reared on a University Campus2021

    • Author(s)
      Yossapol Montira、Yamamoto Miku、Sugiyama Michiyo、Odoi Justice Opare、Omatsu Tsutomu、Mizutani Tetsuya、Ohya Kenji、Asai Tetsuo
    • Journal Title

      Antibiotics

      Volume: 10 Issue: 4 Pages: 432-432

    • DOI

      10.3390/antibiotics10040432

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Continuous prevalence of VEB‐3 extended‐spectrum β‐lactamase‐producing <i>Aeromonas hydrophila</i> in a local river in gifu city, Japan2021

    • Author(s)
      Aratani Tomoki、Koide Nanami、Hayami Kana、Sugiyama Michiyo、Minamoto Toshifumi、Asai Tetsuo
    • Journal Title

      Microbiology and Immunology

      Volume: 65 Issue: 2 Pages: 99-100

    • DOI

      10.1111/1348-0421.12873

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clonal Spread of Quinolone-Resistant Escherichia coli among Sika Deer (Cervus nippon) Inhabiting an Urban City Park in Japan2021

    • Author(s)
      Ikushima Shiori、Torii Harumi、Asano Makoto、Suzuki Masatsugu、Asai Tetsuo
    • Journal Title

      Journal of Wildlife Diseases

      Volume: 57 Issue: 1 Pages: 172-177

    • DOI

      10.7589/jwd-d-19-00005

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ISOLATION AND ANTIMICROBIAL SUSCEPTIBILITIES OF NONTUBERCULOUS MYCOBACTERIA FROM WILDLIFE IN JAPAN2020

    • Author(s)
      Odoi Justice Opare、Ohya Kenji、Moribe Junji、Takashima Yasuhiro、Sawai Kotaro、Taguchi Kyoichiro、Fukushi Hideto、Wada Takayuki、Yoshida Shiomi、Asai Tetsuo
    • Journal Title

      Journal of Wildlife Diseases

      Volume: 56 Issue: 4 Pages: 851-862

    • DOI

      10.7589/2019-10-261

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of fecal samples from Amami rabbits (Pentalagus furnessi) indicates low levels of antimicrobial resistance in Escherichia coli2020

    • Author(s)
      Matsunaga Nonoka、Suzuki Mariko、Andoh Masako、Ijiri Moe、Ishikawa Kemi、Obi Takeshi、Chuma Takehisa、Fujimoto Yoshikazu
    • Journal Title

      European Journal of Wildlife Research

      Volume: 66 Issue: 5 Pages: 84-84

    • DOI

      10.1007/s10344-020-01424-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 野生動物における耐性菌の保有状況2020

    • Author(s)
      浅井 鉄夫
    • Journal Title

      Modern Media

      Volume: 66 Pages: 219-225

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] Antimicrobial susceptibility of <i>Escherichia coli</i> isolates obtained from wild mammals between 2013 and 2017 in Japan2020

    • Author(s)
      ASAI Tetsuo、USUI Masaru、SUGIYAMA Michiyo、IZUMI Kazuhiro、IKEDA Tomoyuki、ANDOH Masako
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 82 Issue: 3 Pages: 345-349

    • DOI

      10.1292/jvms.19-0554

    • NAID

      130007814909

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 野生動物に分布する薬剤耐性菌の生態学2022

    • Author(s)
      浅井鉄夫
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 環境DNA分析を用いた薬剤耐性菌の保菌動物の探索2022

    • Author(s)
      橋本渚、坂田雅之、杉山美千代、浅井鉄夫、源利文
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヤクシカの大腸菌:食中毒起因因子と薬剤耐性について2021

    • Author(s)
      池田智行、Kwon MyoungHyun、明石尚美、安藤匡子
    • Organizer
      第9回屋久島学ソサエティ
    • Related Report
      2021 Annual Research Report
  • [Presentation] 野生動物に分布する薬剤耐性菌2021

    • Author(s)
      浅井鉄夫
    • Organizer
      動物用抗菌剤研究会 第47回シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 野生動物に分布する薬剤耐性菌2021

    • Author(s)
      浅井鉄夫
    • Organizer
      第36回 日本環境感染学会総会・学術集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] One Healthの視点からみた動物由来耐性菌の現状2021

    • Author(s)
      臼井優
    • Organizer
      福岡県One Health 国際フォーラム
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 哺乳類環境DNAメタバーコーディング手法による薬剤耐性菌保菌動物の推定2020

    • Author(s)
      速水花奈, 坂田雅之, 荒谷朋紀, 浅井鉄夫, 源利文
    • Organizer
      第67回日本生態学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 奈良公園のシカ個体群内におけるキノロン耐性大腸菌のクローナル拡散2020

    • Author(s)
      生島 詩織,鳥居 春己,淺野 玄,鈴木 正嗣,浅井 鉄夫
    • Organizer
      第93回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 琵琶湖に流入する河川におけるESBL 産生大腸菌の調査2019

    • Author(s)
      小出 菜々美,浅井 鉄夫
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 岐阜市における節足動物からの腸内細菌科細菌の分離と薬剤感受性2019

    • Author(s)
      浅井 鉄夫
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Transmission of a CTX-M-14/IncI1 plasmid from domestic animals to wild animals in the Gifu University, Japan2019

    • Author(s)
      Montira Yossapol、浅井 鉄夫、杉山 美千代
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] カワウから分離されたEscherichia coliおよびE. albertiiの薬剤感受性2018

    • Author(s)
      小出菜々美、杉山美千代、村上光一、黒田誠、須藤明子、鈴木正嗣、浅井鉄夫
    • Organizer
      第161回日本獣医学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 野生動物に分布する薬剤耐性の現状と課題2018

    • Author(s)
      浅井鉄夫
    • Organizer
      第30回日本臨床微生物学会総会・学術集会
    • Related Report
      2018 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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