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Development of precision induction of organoids based on biomaterial design of fluidic and curved extracellular matrix

Research Project

Project/Area Number 18H04167
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionKyushu University

Principal Investigator

Kidoaki Satoru  九州大学, 先導物質化学研究所, 教授 (10336018)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000)
Fiscal Year 2021: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2020: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2019: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2018: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Keywordsオルガノイド / 流動性足場 / 局面足場 / メカノバイオロジー / 曲面足場 / 動的粘弾性 / メカノバイオマテリアル
Outline of Final Research Achievements

To develop a technique and basic understandings for high reproducible and efficient preparation of organoids, i.e., the budding structures of artificial small organs formed in a culture environment, we systematically investigated the effects of parameters of viscoelastic properties and shape-inducing curvature of scaffolds for organoid culture. The rheological properties of Matrigel, which is commonly used in conventional organoid culture, were modulated by additional cross-linking and mechanical fragmentation of the matrix to determine the optimal viscoelastic property of the matrix for organoid formation. A simple synthetic polymer, PEG, was physically crosslinked with modified polypeptides, and the determined optimal viscoelastic properties were introduced to a laminin-loaded system through regulating the strength of physical crosslinking. This synthetic polymer matrix successfully produced liver organoids more stable than in the Matrigel system.

Academic Significance and Societal Importance of the Research Achievements

オルガノイド形成のための足場材料の力学物性と形状特性の影響に関する基礎研究は関連の分野で近年注目が集まっている。また実用的な観点でも、生物由来材料であるマトリゲルを合成高分子マトリックスに置き換えてオルガノイドを高効率かつ安定して誘導可能な足場材料の開発は強く望まれている状況にある。本研究ではオルガノイドの再現性の高い高効率誘導を可能とするマトリックスの粘弾性特性を特定し、合成高分子マトリックスにおいてその物性を導入することでマトリゲルの代替材料の学理と実際の材料構築に成功し、学術的および社会的意義の両面において本質的な成果を得ている。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (31 results)

All 2022 2021 2020 2019 2018

All Journal Article (13 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 11 results,  Open Access: 6 results) Presentation (17 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Adhesive-ligand-independent cell-shaping controlled by the lateral deformability of a condensed polymer matrix2021

    • Author(s)
      S. Masaike, S. Sasaki, H. Ebata, K. Moriyama, S. Kidoaki
    • Journal Title

      Polymer J

      Volume: 54 Issue: 2 Pages: 211-222

    • DOI

      10.1038/s41428-021-00577-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear activation in dual-durotaxing cells on a matrix with cell-scale stiffness-heterogeneity2021

    • Author(s)
      Kidoaki Satoru、Ebata Hiroyuki、Moriyama Kosuke、Kuboki Thasaneeya、Tsuji Yukie、Sawada Rumi、Sasaki Saori、Okuda Tatsuya、Hamano Kosuke、Kawano Takahito、Yamamoto Aki、Kono Ken、Tanaka Kazusa
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2021.10.30.466554

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of Cell Shaping and Migration on the Matrix with Cell-scale Stiffness-heterogeneity2021

    • Author(s)
      EBATA Hiroyuki、KIDOAKI Satoru
    • Journal Title

      Seibutsu Butsuri

      Volume: 61 Issue: 3 Pages: 152-156

    • DOI

      10.2142/biophys.61.152

    • NAID

      130008045193

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Why epithelial cells collectively move against a traveling signal wave,2020

    • Author(s)
      T. Fukuyama, H. Ebata, Y. Kondo, S. Kidoaki, K. Aoki, Y. T. Maeda
    • Journal Title

      arXiv

      Volume: -

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] Mechanisms of endothelial cell coverage by pericytes: computational modelling of cell wrapping and in vitro experiments.2020

    • Author(s)
      Sugihara K, Sasaki S, Uemura A, Kidoaki S, Miura T.
    • Journal Title

      J R Soc Interface

      Volume: 17 Issue: 162 Pages: 20190739-20190739

    • DOI

      10.1098/rsif.2019.0739

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 間葉系幹細胞の分化抑制培養力学場の設計2020

    • Author(s)
      木戸秋 悟
    • Journal Title

      実験医学増刊号「疾患に挑むメカノバイオロジー」

      Volume: 38 Pages: 1251-1255

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Characterization of 3D matrix conditions for cancer cell migration with elasticity/porosity-independent tunable microfiber gels2020

    • Author(s)
      Daoxiang Huang, Yu Nakamura, Aya Ogata, Satoru Kidoaki
    • Journal Title

      Polymer Journal

      Volume: 52 Issue: 3 Pages: 333-344

    • DOI

      10.1038/s41428-019-0283-3

    • NAID

      40022176448

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Precise design of microwrinkles through the independent regulation of elasticity on the surface and in the bulk of soft hydrogels2020

    • Author(s)
      Saori Sasaki, Satoru Kidoaki
    • Journal Title

      Polymer Journal

      Volume: 52 Issue: 5 Pages: 515-522

    • DOI

      10.1038/s41428-019-0299-8

    • NAID

      40022335642

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] General cellular durotaxis induced with cell-scale heterogeneity of matrix-elasticity2020

    • Author(s)
      Hiroyuki Ebata, Kousuke Moriyama, Thasaneeya Kuboki, Satoru Kidoaki
    • Journal Title

      Biomaterials

      Volume: 203 Pages: 119647-119647

    • DOI

      10.1016/j.biomaterials.2019.119647

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stiffness-optimized drug-loaded matrix for selective capture and elimination of cancer cells2020

    • Author(s)
      D. Huang and S. Kidoaki
    • Journal Title

      J. Drug Deliv. Sci. Technol.

      Volume: 55 Pages: 01414-01414

    • DOI

      10.1016/j.jddst.2019.101414

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hierarchical Development of Motile Polarity in Durotactic Cells Just Crossing an Elasticity Boundary2020

    • Author(s)
      Thasaneeya Kuboki, Hiroyuki Ebata, Tomoki Matsuda, Yoshiyuki Arai, Takeharu Nagai, Satoru Kidoaki
    • Journal Title

      Cell Structure and Function

      Volume: 45 Issue: 1 Pages: 33-43

    • DOI

      10.1247/csf.19040

    • NAID

      130007801487

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells2018

    • Author(s)
      M. Iwashita, H. Ohta, T. Fujisawa, M. Cho, M. Ikeya, S. Kidoaki and Y. Kosodo
    • Journal Title

      Sci. Rep.

      Volume: 9 Issue: 1 Pages: 3068-3068

    • DOI

      10.1038/s41598-018-38395-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cellular durotaxis revisited: Initial-position-dependent determination of the threshold stiffness gradient to induce durotaxis2018

    • Author(s)
      K. Moriyama and S. Kidoaki
    • Journal Title

      Langmuir

      Volume: 未定 Issue: 23 Pages: 7478-7486

    • DOI

      10.1021/acs.langmuir.8b02529

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] マトリックスメカノバイオロジー2021

    • Author(s)
      木戸秋 悟
    • Organizer
      RIMS Tutorial Seminar
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] オルガノイド形成のための足場界面ミクロレオロジーの解析2020

    • Author(s)
      岩下 皇蔵、江端 宏之、藤田 彩乃、辻 ゆきえ、木戸秋 悟
    • Organizer
      第59回日本生体医工学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 管状足場における間葉系幹細胞の曲率依存的動態の解析2020

    • Author(s)
      藤田 彩乃、木戸秋 悟
    • Organizer
      第59回日本生体医工学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オルガノイド安定形成を誘導するマトリケ?ル粘弾性条件の特定2020

    • Author(s)
      岩下皇藏、江端宏之、辻ゆきえ、木戸秋悟
    • Organizer
      第32回高分子ゲル研究討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] S1P修飾ハイドロゲルを用いたMuse細胞ホーミング及び力学場応答性の解析2019

    • Author(s)
      郭 蕾、辻ゆきえ、木戸秋 悟
    • Organizer
      第56回化学関連支部合同九州大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞培養ハイドロゲル表面リンクルの精密マルチスケール制御2019

    • Author(s)
      佐々木 沙織,木戸秋悟
    • Organizer
      日本機械学会 第 30 回バイオフロンティア講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞スケール不均一弾性場におけるデュロタクシスの一般挙動2019

    • Author(s)
      江端宏之、森山幸祐、久保木タッサニーヤー、木戸秋悟
    • Organizer
      第一回メカノバイオロジー研究会2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 弾性パターニング場におけるドメインサイズ依存的な硬領域指向性細胞運動2019

    • Author(s)
      江端 宏之, 森山幸祐, 久保木タッサニーヤー, 木戸秋 悟
    • Organizer
      日本物理学会2019秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] iPS cells show mechanotactic accumulation, enhanced proliferation and higher expression of stemness marker in optimal region of matrix elasticity2019

    • Author(s)
      王 夢繁、木戸秋悟
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Homing and mechano-response of Muse cells analyzed on S1P-modified hydrogel with tunable elasticity2019

    • Author(s)
      郭蕾、木戸秋悟
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Evaluation of cell mechanotransduction regulation via matrix with lateral deformation characteristics2019

    • Author(s)
      政池彩雅、木戸秋悟
    • Organizer
      第57回日本生物物理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 非一様弾性場・非定住遊走による間葉系幹細胞のエクササイズ培養2019

    • Author(s)
      "木戸秋 悟、森山 幸祐、久保木 タッサニーヤー、澤田 留美、辻 ゆきえ、江端 宏之、佐々木 沙織、山本 安希、田中 和沙、河野 健 "
    • Organizer
      第68回高分子討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Exercising mesenchymal stem cells through nomadic culture on heterogeneous field of matrix elasticity2019

    • Author(s)
      Satoru Kidoaki, Kouske Moriyama, Thasaneeya Kuboki, Rumi Sawada, Yukie Tsuji, Hiroyuki Ebata, Saori Sasaki, Aya Yamamoto, Ken Kono, Kazusa Tanaka
    • Organizer
      Cell Physics 2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] Heterogeneous field of matrix elasticity to exercise mesenchymal stem cells through their nomadic migrations2019

    • Author(s)
      Satoru Kidoaki
    • Organizer
      Okinawa Colloids 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微視的培養力学場設計に基づく細胞操作技術2019

    • Author(s)
      木戸秋 悟
    • Organizer
      日本動物実験代替法学会 第32回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] "Traction force dynamics of mesenchymal stem cells on micro-elastically patterned hydrogels "2019

    • Author(s)
      Hiroyuki Ebata, Satoru Kidoaki
    • Organizer
      2nd G'L'owing Polymer Symposium in KANTO
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞形態振動を誘起する接着海面の垂直/水平変形特性の動的制御2019

    • Author(s)
      政池 彩雅、木戸秋 悟
    • Organizer
      第32回バイオエンジニアリング講演会
    • Related Report
      2019 Annual Research Report
  • [Book] Material-based Mechanobiology2022

    • Author(s)
      Jun Nakanishi, Koichiro Uto Ed.
    • Total Pages
      450
    • Publisher
      Royal Society of Chemistry
    • ISBN
      183916185X
    • Related Report
      2021 Annual Research Report

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Published: 2018-04-23   Modified: 2023-01-30  

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