| Project/Area Number |
18J11639
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
Biofunction/Bioprocess
|
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| Research Institution | The University of Tokyo (2019)
Yokohama National University (2018) |
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Principal Investigator |
MYASNIKOVA Dina 東京大学, 大学院情報理工学系研究科, 特別研究員(PD)
|
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| Project Period (FY) |
2018-04-25 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2019: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
|
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| Keywords | pancreas / insulin / ROS / glucose / glucotoxicity / oxidative stress / tissue engineering / antioxidant |
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| Outline of Annual Research Achievements |
The goal of my research was to fabricate bioartificial pancreatic islets and optimise their culture conditions. The first key point of my work is the use of oxygen permeable PDMS-chip to fabricate pancreatic spherical aggregates (spheroids), which provided cells with adequate oxygenation and prevented development of hypoxic core during long culture (1 week).
The next key point is the use of the antioxidant ascorbic acid-2-phosphate in culture medium, which protects cells from reactive oxygen species generated due to the fact that in vitro conditions differ from the optimal in vivo ones.
The third key point is the glucose concentration in culture medium. Although monolayer is culture in high glucose, my investigation showed that lower glucose concentration is beneficial for spheroid culture.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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