Cross-organ HSC trafficking during definitive hematopoiesis formation
| Project/Area Number |
18J20690
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 国内 |
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| Research Field |
Developmental biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
瀬崎 真衣子 熊本大学, 医学教育部, 特別研究員(DC1)
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| Project Period (FY) |
2018-04-25 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2020: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2019: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2018: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | definitive hematopoiesis / hematopoietic stem cells / stem cell heterogeneity / bone marrow / HSC trafficking / 3D whole-organ imaging / lineage tracing / mathematical modeling / hematopoietic switching / deep-learning analysis / EC remodeling |
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| Outline of Annual Research Achievements |
In this study, the cellular and molecular mechanism of hematopoietic stem cell (HSC) trafficking that occurs during definitive hematopoiesis formation was investigated in the fetal to neonatal mouse. Single-cell RNA-sequencing of P2 HSCs isolated from the bone marrow, liver and spleen revealed HSC heterogeneity. PCA analysis showed 3 distinct clusters of HSCs with unique HSC marker expression (varying levels of cKit, Sca-1, CD150 mean fluorescence intensity) and differentially expressed genes. Our functional analysis via in vitro CFU-F and in vivo 10-cell transplantation into lethally irradiated mice revealed striking differences among these 3 groups in their ability to generate colonies and donor chimerism. We are now investigating the biological significance of these HSC groups and their contribution to adult hematopoiesis via lineage tracing and knock-out or knock-down studies.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(13 results)
