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BAT thermogenesis-triggered organ cross-talk through the exosomal microRNA

Research Project

Project/Area Number 18J21697
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section国内
Research Field Metabolomics
Research InstitutionHokkaido University

Principal Investigator

BARIUAN JUSSIAEA VALENTE  北海道大学, 獣医学院, 特別研究員(DC1)

Project Period (FY) 2018-04-25 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2020: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2019: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
KeywordsmicroRNA-122 / skeletal muscle / C2C12 cells / interleukin-6 / time-response / myostatin / brown adipose tissue / uncoupling protein 1 / cold exposure / microRNA / microRNA 122 (miR-122) / Uncoupling protein 1 / BAT activation / nonessential fatty acids / beta-adrenergic receptor agonist / liver
Outline of Annual Research Achievements

To understand the effect of batokines: interleukin-6 (IL-6)and myostatin (GDF 8), on microRNA 122 (miR-122) expression in myocytes, C2C12 myoblasts differentiated for 14 to 16 days and showing myotube formation were treated with two different concentrations of each of the mentioned batokines for 6 hours. After treatment, the microRNA of the cells were harvested and measured. 25ng of IL-6 significantly increased C2C12 miR-122 expression (%) unlike the higher 100ng treatment concentration which only increased the C2C12 by % compared to the control group.
To determine the potency of IL-6 effect on miR-122 expression in C2C12 cells, I checked the time- and dose-responses. The effects of 5ng, 10ng and 25ng IL-6 doses on C2C12 were not significantly different from each other. In contrast, a significant difference in the time-response to IL-6 treatment was observed. After 2 hours, miR-122 expression was 56% higher than at the start of the treatment. At 4 hours after treatment, a 53% increase in the miR-122 expression was observed whereas after 8 hours, there was a 31% drop in the miR-122 expression in the C2C12 cells. This indicates a significant time-response of myocyte miR-122 expression to IL-6.
On the other hand, GDF8 treatment did not produce any significant effects on C2C12 miR-122 expression

Research Progress Status

令和2年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和2年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • Research Products

    (8 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Chronic low-dose exposure to imidacloprid potentiates high fat diet-mediated liver steatosis in C57BL/6J male mice2021

    • Author(s)
      NIMAKO Collins、IKENAKA Yoshinori、OKAMATSU-OGURA Yuko、BARIUAN Jussiaea V.、KOBAYASHI Atsushi、YAMAZAKI Ryo、TAIRA Kumiko、HOSHI Nobuhiko、HIRANO Tetsushi、NAKAYAMA Shouta M. M.、ISHIZUKA Mayumi
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 83 Issue: 3 Pages: 487-500

    • DOI

      10.1292/jvms.20-0479

    • NAID

      130008021217

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Attenuation of carrageenan-induced hind paw edema and plasma TNF-α level by Philippine stingless bee (<i>Tetragonula biroi</i> Friese) propolis2021

    • Author(s)
      Calimag KPD, Arbis CCH, Collantes TMA, Bariuan JV, Ang MJC, Cervancia CA, Desamero MJM, Estacio MAC.
    • Journal Title

      Experimental Animals

      Volume: 70 Issue: 2 Pages: 185-193

    • DOI

      10.1538/expanim.20-0118

    • NAID

      130008037408

    • ISSN
      0007-5124, 1341-1357, 1881-7122
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cold Exposure Increases Circulating miR-122 Levels via UCP1-Dependent Mechanism in Mice2020

    • Author(s)
      J.V. Bariuan, Y. Okamatsu-Ogura, A. Tsubota, S. Matsuoka, M. Saito, K. Kimura.
    • Journal Title

      Japanese Journal of Veterinary Research

      Volume: 68 Issue: 3 Pages: 187-195

    • DOI

      10.14943/jjvr.68.3.187

    • NAID

      120006887731

    • ISSN
      0047-1917
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of circulating exosomal miR-122 levels with BAT activity in healthy humans2019

    • Author(s)
      Okamatsu-Ogura Yuko、Matsushita Mami、Bariuan Jussiaea Valente、Nagaya Kazuki、Tsubota Ayumi、Saito Masayuki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 13243-13243

    • DOI

      10.1038/s41598-019-49754-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of brown adipose tissue in body temperature control during the early postnatal period in Syrian hamsters and mice2019

    • Author(s)
      Tsubota, A., Okamatsu-Ogura, Y., Bariuan, J.V., Mae, J., Matsuoka, S., Nio-Kobayashi, J., and Kimura, K.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 10 Pages: 1461-1467

    • DOI

      10.1292/jvms.19-0371

    • NAID

      130007732113

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 寒冷暴露は褐色脂肪の熱産生タンパク質 UCP1依存的に血中 miR- 122を上昇させる2020

    • Author(s)
      Jussiaea Valente Bariuan、岡松優子、松岡慎也、坪田あゆみ、斉藤昌之、木村和弘
    • Organizer
      第163回日本獣医学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Cold-induced activation of BAT thermogenesis increases circulating miR-122 level possibly through the secretion from muscle2019

    • Author(s)
      Jussiaea Valente Bariuan
    • Organizer
      97th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] Circulating microRNA-122 (miR-122): Do adipose tissues really controbute?2018

    • Author(s)
      Jussiaea Valente BARIUAN
    • Organizer
      The 6th Sapporo Summer Symposium for One Health (SaSSOH 2018) supported by Hokkaido University Program for Leading Graduate School
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research

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Published: 2018-05-01   Modified: 2024-03-26  

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