| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2020: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2019: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2018: ¥800,000 (Direct Cost: ¥800,000)
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| Outline of Annual Research Achievements |
To understand the effect of batokines: interleukin-6 (IL-6)and myostatin (GDF 8), on microRNA 122 (miR-122) expression in myocytes, C2C12 myoblasts differentiated for 14 to 16 days and showing myotube formation were treated with two different concentrations of each of the mentioned batokines for 6 hours. After treatment, the microRNA of the cells were harvested and measured. 25ng of IL-6 significantly increased C2C12 miR-122 expression (%) unlike the higher 100ng treatment concentration which only increased the C2C12 by % compared to the control group.
To determine the potency of IL-6 effect on miR-122 expression in C2C12 cells, I checked the time- and dose-responses. The effects of 5ng, 10ng and 25ng IL-6 doses on C2C12 were not significantly different from each other. In contrast, a significant difference in the time-response to IL-6 treatment was observed. After 2 hours, miR-122 expression was 56% higher than at the start of the treatment. At 4 hours after treatment, a 53% increase in the miR-122 expression was observed whereas after 8 hours, there was a 31% drop in the miR-122 expression in the C2C12 cells. This indicates a significant time-response of myocyte miR-122 expression to IL-6.
On the other hand, GDF8 treatment did not produce any significant effects on C2C12 miR-122 expression
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