• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Integrated thermophysical property analysis of live cells by photothermal microscopy

Research Project

Project/Area Number 18K03561
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 13040:Biophysics, chemical physics and soft matter physics-related
Research InstitutionWakayama University

Principal Investigator

Jun Miyazaki  和歌山大学, システム工学部, 講師 (50467502)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords光熱変換顕微鏡 / 生細胞イメージング / 熱伝導 / ミトコンドリア / ミクロ熱物性 / 無標識イメージング / リソソーム / 光熱変換顕微計測法 / 熱物性計測 / 極微スケール熱伝導 / 生細胞
Outline of Final Research Achievements

This study aims to develop an optical method for measuring heat conduction inside a cell based on photothermal microscopy. Using this system, we aimed to reveal the relationship between heat and cellular function and the cause of the temperature imbalance observed inside a cell.
This project developed a novel photodetector and optical setup that enable high-sensitivity and high-fidelity photothermal imaging of mitochondria in live cells. We demonstrated the effectiveness of the simultaneous multi-frequency method for measuring the thermal conductivity of moving objects. We identified cellular organelles observed by photothermal microscope and examined optimal culture conditions for selective imaging of mitochondria. Subsequently, thermal conductivity measurements in the vicinity of mitochondria were performed.

Academic Significance and Societal Importance of the Research Achievements

がん細胞などの病態細胞では亢進した熱発生があること、細胞活性に依存して熱伝導率が変化することが報告されており、本研究で開発した熱物性計測法は新規診断,治療法の開発に貢献すると期待できる。さらにナノテクノロジーを基盤とするマイクロマシン、ナノマシンの高効率・高速動作にはまさつの制御と熱散逸過程の把握がキーポイントとなり、本研究で提案したミクロな熱物性測定法はその実現に貢献できる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (10 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results)

  • [Journal Article] 光熱変換顕微鏡による生細胞の無標識イメージング2020

    • Author(s)
      宮崎 淳
    • Journal Title

      フォトニクスニュース

      Volume: 6

    • Related Report
      2020 Research-status Report
  • [Journal Article] 生細胞の無標識イメージング -光熱変換顕微鏡で見るミトコンドリアとリソソーム-2020

    • Author(s)
      宮崎 淳
    • Journal Title

      光学

      Volume: 49 Pages: 229-229

    • Related Report
      2020 Research-status Report
  • [Journal Article] Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy2019

    • Author(s)
      Miyazaki Jun、Toumon Yasunobu
    • Journal Title

      Biomedical Optics Express

      Volume: 10 Issue: 11 Pages: 5852-5852

    • DOI

      10.1364/boe.10.005852

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Experimental evaluation of temperature increase and associated detection sensitivity in shot noise-limited photothermal microscopy2019

    • Author(s)
      Jun Miyazaki and Yasunobu Toumon
    • Journal Title

      Optics Communications

      Volume: 430 Pages: 170-175

    • DOI

      10.1016/j.optcom.2018.08.007

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 光熱変換顕微鏡を用いた液中単一単層カーボンナノチューブの吸収イメージングと分散・凝集状態の観察2022

    • Author(s)
      宮崎淳、近藤亮介
    • Organizer
      第68回応用物理学会春季学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 光熱変換顕微鏡による動物細胞の無標識オートファジー計測2021

    • Author(s)
      宮崎淳、岡本浩二
    • Organizer
      第83回応用物理学会秋季学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Label-fee dynamic imaging of mitochondria and related organelles inside live cells with simultaneous dual-wavelength photothermal microscopy2019

    • Author(s)
      J. Miyazaki and Y. Toumon
    • Organizer
      OPTICS and PHOTONICS International Congress 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Multi-Wavelength Absorption Contrast Imaging of Individual Single-Wall Carbon Nanotubes with Photothermal Microscopy2019

    • Author(s)
      Y. Ishikawa and J. Miyazaki,
    • Organizer
      International Symposium on Imaging, Sensing, and Optical Memory 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Photothermal Microscopy for High-sensitivity Absorption Imaging of Biological Tissues2019

    • Author(s)
      J. Miyazaki
    • Organizer
      International Symposium on Imaging, Sensing, and Optical Memory 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 光熱変換顕微鏡による生物組織の高感度光吸収イメージング2019

    • Author(s)
      宮崎淳
    • Organizer
      レーザー学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 光熱変換顕微鏡によるミトコンドリアダイナミクスの無標識イメージング2019

    • Author(s)
      宮崎淳、東門泰伸
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] 同時2色光熱変換顕微イメージングによる単一単層カーボンナノチューブの解析2019

    • Author(s)
      石川祐栽、宮崎淳
    • Organizer
      第66回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] Label-fee dynamic imaging of mitochondria and related organelles inside live cells with simultaneous dual-wavelength photothermal microscopy2019

    • Author(s)
      Jun Miyazaki and Yasunobu Toumon
    • Organizer
      OPTICS&PHOTONICS International Conference 2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 同時2色光熱変換顕微イメージングによる単一単層カーボンナノチューブの分析2018

    • Author(s)
      石川祐栽、宮崎淳
    • Organizer
      第29回 光物性研究会
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi