| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
This study aims to develop an optical method for measuring heat conduction inside a cell based on photothermal microscopy. Using this system, we aimed to reveal the relationship between heat and cellular function and the cause of the temperature imbalance observed inside a cell.
This project developed a novel photodetector and optical setup that enable high-sensitivity and high-fidelity photothermal imaging of mitochondria in live cells. We demonstrated the effectiveness of the simultaneous multi-frequency method for measuring the thermal conductivity of moving objects. We identified cellular organelles observed by photothermal microscope and examined optimal culture conditions for selective imaging of mitochondria. Subsequently, thermal conductivity measurements in the vicinity of mitochondria were performed.
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