Intracellular molecular mechanisms of selective killing of cancer cells by plasma-activated solutions

• Project/Area Number: 18K03599
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 14030:Applied plasma science-related
• Research Institution: Nagoya University
• Principal Investigator: Hiromasa Tanaka 名古屋大学, 低温プラズマ科学研究センター, 教授 (00508129)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 低温プラズマ / プラズマ活性溶液 / プラズマ生命科学 / プラズマがん治療 / 脳腫瘍培養細胞 / 遺伝子発現

Outline of Final Research Achievements

Low-temperature plasma has been studied for medical applications. We had focused on the plasma cancer treatments, and we have previously invented plasma-activated solutions. In this study, we used plasma-activated medium (PAM) and plasma-activated Ringer’s lactate solution (PAL) for plasma cancer treatments. We found that PAM induced oxidative stress-dependent cell death on glioblastoma brain tumor cells while PAL induced oxidative stress-independent cell death on glioblastoma cells. These results suggest that plasma-activated solutions might be useful for cancer drug resistant cancer therapy.

Academic Significance and Societal Importance of the Research Achievements

近年、低温プラズマの医療応用研究が盛んに行われてきており、低温プラズマによるがん治療は最も魅力的かつ挑戦的な研究分野として世界中の多くの研究者が携わっている。低温プラズマを直接がんに照射するのみならず、低温プラズマを照射した溶液（プラズマ活性溶液）を用いたがん治療法を我々は提案した。本研究で我々は更に様々なプラズマ活性によるがん細胞の細胞死の機構を調べ、プラズマ活性溶液ごとにその機構が異なることを突き止めた。本成果により将来プラズマ活性溶液が抗癌剤に耐性を有するがん治療にも適用できるようになるかもしれない。

Research Products

• [Journal Article] L-Dehydroascorbate efficiently degrades non-thermal plasma-induced hydrogen peroxide (2021)
  • Author(s): Okazaki Yasumasa、Ishidzu Yuuri、Ito Fumiya、Tanaka Hiromasa、Hori Masaru、Toyokuni Shinya
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 700 Pages: 108762-108762
  • DOI: 10.1016/j.abb.2021.108762
  • Peer Reviewed
• [Journal Article] Preclinical Verification of the Efficacy and Safety of Aqueous Plasma for Ovarian Cancer Therapy (2021)
  • Author(s): Nakamura Kae、Yoshikawa Nobuhisa、Mizuno Yuko、Ito Miwa、Tanaka Hiromasa、Mizuno Masaaki、Toyokuni Shinya、Hori Masaru、Kikkawa Fumitaka、Kajiyama Hiroaki
  • Journal Title: Cancers Volume: 13 Issue: 5 Pages: 1141-1141
  • DOI: 10.3390/cancers13051141
  • Peer Reviewed / Open Access
• [Journal Article] Inside Front Cover: Plasma Process. Polym. 1/2021 (2021)
  • Author(s): Hashizume Hiroshi、Kitano Hidemi、Mizuno Hiroko、Abe Akiko、Yuasa Genki、Tohno Satoe、Tanaka Hiromasa、Ishikawa Kenji、Matsumoto Shogo、Sakakibara Hitoshi、Nikawa Susumu、Maeshima Masayoshi、Mizuno Masaaki、Hori Masaru
  • Journal Title: Plasma Processes and Polymers Volume: 18 Issue: 1 Pages: 2170002-2170002
  • DOI: 10.1002/ppap.202170002
  • Peer Reviewed
• [Journal Article] Improvement of yield and grain quality by periodic cold plasma treatment with rice plants in a paddy field (2020)
  • Author(s): Hashizume Hiroshi、Kitano Hidemi、Mizuno Hiroko、Abe Akiko、Yuasa Genki、Tohno Satoe、Tanaka Hiromasa、Ishikawa Kenji、Matsumoto Shogo、Sakakibara Hitoshi、Nikawa Susumu、Maeshima Masayoshi、Mizuno Masaaki、Hori Masaru
  • Journal Title: Plasma Processes and Polymers Volume: 18 Issue: 1 Pages: 2000181-2000181
  • DOI: 10.1002/ppap.202000181
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Non-thermal plasma?activated lactate solution kills U251SP glioblastoma cells in an innate reductive manner with altered metabolism (2020)
  • Author(s): Ishikawa Kenji、Hosoi Yugo、Tanaka Hiromasa、Jiang Li、Toyokuni Shinya、Nakamura Kae、Kajiyama Hiroaki、Kikkawa Fumitaka、Mizuno Masaaki、Hori Masaru
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 688 Pages: 108414-108414
  • DOI: 10.1016/j.abb.2020.108414
  • Peer Reviewed
• [Journal Article] Laser-induced-plasma-activated medium enables killing of HeLa cells (2020)
  • Author(s): Kurokawa Yukihiro、Takeda Keigo、Ishikawa Kenji、Tanaka Hiromasa、Hori Masaru
  • Journal Title: Applied Physics Express Volume: 13 Issue: 10 Pages: 106001-106001
  • DOI: 10.35848/1882-0786/abb68c
  • Peer Reviewed
• [Journal Article] Adjusted multiple gases in the plasma flow induce differential antitumor potentials of plasma‐activated solutions (2020)
  • Author(s): Nakamura Kae、Yoshikawa Nobuhisa、Yoshihara Masato、Ikeda Yoshiki、Higashida Akihiro、Niwa Akihiro、Jindo Takahiro、Tanaka Hiromasa、Ishikawa Kenji、Mizuno Masaaki、Toyokuni Shinya、Hori Masaru、Kikkawa Fumitaka、Kajiyama Hiroaki
  • Journal Title: Plasma Processes and Polymers Volume: 17 Issue: 10 Pages: 1900259-1900259
  • DOI: 10.1002/ppap.201900259
  • Peer Reviewed / Open Access
• [Journal Article] Atomic oxygen radical‐induced intracellular oxidization of mould spore cells (2020)
  • Author(s): Tanaka Yuta、Oh Jun‐Seok、Hashizume Hiroshi、Ohta Takayuki、Kato Masashi、Hori Masaru、Ito Masafumi
  • Journal Title: Plasma Processes and Polymers Volume: 17 Issue: 10 Pages: 2000001-2000001
  • DOI: 10.1002/ppap.202000001
  • Peer Reviewed
• [Journal Article] Special issue: Advanced applications of plasmas in Life Sciences 2020 (2020)
  • Author(s): Favia Pietro、Sardella Eloisa、Tanaka Hiromasa
  • Journal Title: Plasma Processes and Polymers Volume: 17 Issue: 10 Pages: 2070028-2070028
  • DOI: 10.1002/ppap.202070028
  • Peer Reviewed
• [Journal Article] Plasma-activated medium promotes autophagic cell death along with alteration of the mTOR pathway (2020)
  • Author(s): Yoshikawa Nobuhisa、Liu Wenting、Nakamura Kae、Yoshida Kosuke、Ikeda Yoshiki、Tanaka Hiromasa、Mizuno Masaaki、Toyokuni Shinya、Hori Masaru、Kikkawa Fumitaka、Kajiyama Hiroaki
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1614-1614
  • DOI: 10.1038/s41598-020-58667-3
  • Peer Reviewed / Open Access
• [Journal Article] Oxidative stress-dependent and -independent death of glioblastoma cells induced by non-thermal plasma-exposed solutions (2019)
  • Author(s): Tanaka Hiromasa、Mizuno Masaaki、Katsumata Yuko、Ishikawa Kenji、Kondo Hiroki、Hashizume Hiroshi、Okazaki Yasumasa、Toyokuni Shinya、Nakamura Kae、Yoshikawa Nobuhisa、Kajiyama Hiroaki、Kikkawa Fumitaka、Hori Masaru
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 13657-13657
  • DOI: 10.1038/s41598-019-50136-w
  • Peer Reviewed / Open Access
• [Journal Article] -Dehydroascorbic acid recycled by thiols efficiently scavenges non-thermal plasma-induced hydroxyl radicals (2019)
  • Author(s): Okazaki Yasumasa、Tanaka Hiromasa、Hori Masaru、Toyokuni Shinya
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 669 Pages: 87-95
  • DOI: 10.1016/j.abb.2019.05.019
  • Peer Reviewed
• [Journal Article] Gene Expression of Osteoblast-like Cells on Carbon-Nanowall as Scaffolds during Incubation with Electrical Stimulation (2019)
  • Author(s): Ichikawa Tomonori、Kondo Hiroki、Ishikawa Kenji、Tsutsumi Takayoshi、Tanaka Hiromasa、Sekine Makoto、Hori Masaru
  • Journal Title: ACS Applied Bio Materials Volume: 2 Issue: 7 Pages: 2698-2702
  • DOI: 10.1021/acsabm.9b00178
  • Peer Reviewed
• [Journal Article] Plasma-activated solution alters the morphological dynamics of supported lipid bilayers observed by high-speed atomic force microscopy (2019)
  • Author(s): Yamaoka Sotaro、Kondo Hiroki、Hashizume Hiroshi、Ishikawa Kenji、Tanaka Hiromasa、Hori Masaru
  • Journal Title: Applied Physics Express Volume: 12 Issue: 6 Pages: 066001-066001
  • DOI: 10.7567/1882-0786/ab1a58
  • NAID: 210000155782
  • Peer Reviewed
• [Journal Article] Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner (2019)
  • Author(s): Sato Kotaro、Shi Lei、Ito Fumiya、Ohara Yuuki、Motooka Yashiro、Tanaka Hiromasa、Mizuno Masaaki、Hori Masaru、Hirayama Tasuku、Hibi Hideharu、Toyokuni Shinya
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 65 Issue: 1 Pages: 8-15
  • DOI: 10.3164/jcbn.18-91
  • NAID: 130007672104
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access
• [Journal Article] A 65-nm CMOS Fully Integrated Analysis Platform Using an On-Chip Vector Network Analyzer and a Transmission-Line-Based Detection Window for Analyzing Circulating Tumor Cell and Exosome (2019)
  • Author(s): Niitsu Kiichi、Nakanishi Taiki、Murakami Shunya、Matsunaga Maya、Kobayashi Atsuki、Karim Nissar Mohammad、Ito Jun、Ozawa Naoya、Hase Tetsunari、Tanaka Hiromasa、Sato Mitsuo、Kondo Hiroki、Ishikawa Kenji、Odaka Hidefumi、Hasegawa Yoshinori、Hori Masaru、Nakazato Kazuo
  • Journal Title: IEEE Transactions on Biomedical Circuits and Systems Volume: 13 Issue: 2 Pages: 470-479
  • DOI: 10.1109/tbcas.2018.2882472
  • Peer Reviewed / Open Access
• [Journal Article] Non-thermal plasma-activated medium modified metabolomic profiles in the glycolysis of U251SP glioblastoma (2019)
  • Author(s): Kurake Naoyuki、Ishikawa Kenji、Tanaka Hiromasa、Hashizume Hiroshi、Nakamura Kae、Kajiyama Hiroaki、Toyokuni Shinya、Kikkawa Fumitaka、Mizuno Masaaki、Hori Masaru
  • Journal Title: Archives of Biochemistry and Biophysics Volume: 662 Pages: 83-92
  • DOI: 10.1016/j.abb.2018.12.001
  • Peer Reviewed
• [Journal Article] Low-autofluorescence fluoropolymer membrane filters for cell filtration (2018)
  • Author(s): Kihara Naoto、Kuboyama Daiki、Onoshima Daisuke、Ishikawa Kenji、Tanaka Hiromasa、Ozawa Naoya、Hase Tetsunari、Koguchi Ryohei、Yukawa Hiroshi、Odaka Hidefumi、Hasegawa Yoshinori、Baba Yoshinobu、Hori Masaru
  • Journal Title: Japanese Journal of Applied Physics Volume: 57 Issue: 6S2 Pages: 06JF03-06JF03
  • DOI: 10.7567/jjap.57.06jf03
  • NAID: 210000149208
  • Peer Reviewed / Open Access
• [Journal Article] Molecular mechanisms of non-thermal plasma-induced effects in cancer cells (2018)
  • Author(s): Tanaka Hiromasa、Mizuno Masaaki、Ishikawa Kenji、Toyokuni Shinya、Kajiyama Hiroaki、Kikkawa Fumitaka、Hori Masaru
  • Journal Title: Biological Chemistry Volume: 400 Issue: 1 Pages: 87-91
  • DOI: 10.1515/hsz-2018-0199
  • NAID: 120006577057
  • Peer Reviewed
• [Journal Article] New Hopes for Plasma-Based Cancer Treatment (2018)
  • Author(s): Tanaka Hiromasa、Mizuno Masaaki、Ishikawa Kenji、Toyokuni Shinya、Kajiyama Hiroaki、Kikkawa Fumitaka、Hori Masaru
  • Journal Title: Plasma Volume: 1 Issue: 1 Pages: 150-155
  • DOI: 10.3390/plasma1010014
  • Peer Reviewed
• [Journal Article] Cytotoxic effects of plasma-irradiated fullerenol (2018)
  • Author(s): Kanno Daiki、Tanaka Hiromasa、Ishikawa Kenji、Hashizume Hiroshi、Hori Masaru
  • Journal Title: Journal of Physics D: Applied Physics Volume: 51 Issue: 37 Pages: 375401-375401
  • DOI: 10.1088/1361-6463/aad510
  • Peer Reviewed
• [Journal Article] Liquid dynamics in response to an impinging low-temperature plasma jet (2018)
  • Author(s): Brubaker T R、Ishikawa K、Kondo H、Tsutsumi T、Hashizume H、Tanaka H、Knecht S D、Bilen S G、Hori M
  • Journal Title: Journal of Physics D: Applied Physics Volume: 52 Issue: 7 Pages: 075203-075203
  • DOI: 10.1088/1361-6463/aaf460
  • Peer Reviewed
• [Presentation] Plasma activated Ringer's lactate solution affected cellular respiratory system on HeLa cells. (2020)
  • Author(s): Hiromasa Tanaka, Shogo Maeda, Kae Nakamura, Hiroshi Hashizume, Kenji Ishikawa, Mikako Ito, Kinji Ohno, Masaaki Mizuno, Shinya Toyokuni, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori.
  • Organizer: 73th Annual Gaseous Electronics Virtual Conference
  • Int'l Joint Research
• [Presentation] 様々なプラズマ活性溶液による細胞死の作用機序 (2020)
  • Author(s): 田中 宏昌、堀 勝
  • Organizer: 第81回応用物理学会秋季学術講演会
  • Invited
• [Presentation] Plasma activated media for cancer treatment (2019)
  • Author(s): H. Tanaka & M. Hori
  • Organizer: 6th International Workshop on Plasma for Cancer Treatment (IWPCT2019) Mini-course: Plasma & Cancer , Antwerp, Belgium , 2019/04/01
  • Int'l Joint Research / Invited
• [Presentation] Plasma-activated fluids as a novel treatment option for peritoneal metastasis from gastric/pancreatic cancer (2019)
  • Author(s): Y.Kodera, S.Yamada, K.Torii, N.Hattori, S.Takeda, Y.Sato, H.Tanaka, M.Mizuno and M.Hori
  • Organizer: 6th International Workshop on Plasma for Cancer Treatment (IWPCT2019) , Antwerp, Belgium , 2019/04/02
  • Int'l Joint Research / Invited
• [Presentation] Gene expression analyses revealed the differences of intracellular molecular mechanisms of PAM- and PAL-induced cell death (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Yuko Katsumata, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Organizer: "The 12th Asian-European International Conference on Plasma Surface Engineering (AEPSE 2019)" , Jeju-do, Korea , 2019/09/03
  • Int'l Joint Research / Invited
• [Presentation] Effects of Plasma Treatment on Plant Cultivation (2019)
  • Author(s): H. Hashizume, H. Kitano, S.Mitsumoto, G. Yuasa, S. Tohno, M.Kojima, Y. Takebayashi, H. Mizuno, S. Kinoshita, K. Tsubota, H.Tanaka, K. Ishikawa, M.Ito, H. Sakakibara, M. Maeshima, T. Okuma, S.Nikawa, M. Mizuno, and M. Hori
  • Organizer: 14th Asian Pacific Physics conference , Sarawak, Malaysia , 2019/11/20
  • Int'l Joint Research / Invited
• [Presentation] Plasma cancer treatments in the current plasma medical science (2019)
  • Author(s): H. Tanaka, M. Mizuno, K. Ishikawa, S. Toyokuni, H. Kajiyama, F. Kikkawa and M. Hori
  • Organizer: "29th Annual Meeting of MRS-Japan 2019" , 横浜情報文化センター、横浜市開港記念会館 , 2019/11/29
  • Int'l Joint Research / Invited
• [Presentation] ene expression dynamics of glioblastoma cells in plasma-activated medium and plasma-activated Ringer’s lactate solution (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Makoto Sekine, Hiroki Kondo, Hiroshi Hashizume, Takayoshi Tsutsumi, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Shinya Toyokuni, Fumitaka Kikkawa, Masaru Hori
  • Organizer: 24th International Symposium on Plasma Chemistry (ISPC24) , Naples Italy , 2019/06/12
  • Int'l Joint Research
• [Presentation] Enhancement of alcohol production of budding yeasts by treatment of non-equilibrium atmospheric pressure plasma (2019)
  • Author(s): Shogo Matsumura, Kenji Ishikawa, Hiromasa Tanaka, Hiroshi Hashizume, Masafumi Ito, Masaru Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases(XXXIV ICPIG)/10th International Conference on Reactive Plasmas(ICRP-10) , Sapporo, Hokkaido , 2019/07/16
  • Int'l Joint Research
• [Presentation] Differences of intracellular molecular mechanisms of cell death between plasma-activated medium-treated glioblastoma cells and plasma-activated Ringer’s lactate solution-treated glioblastoma cells (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Yuko Katsumata, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
  • Organizer: XXXIV International Conference on Phenomena in Ionized Gases(XXXIV ICPIG)/10th International Conference on Reactive Plasmas(ICRP-10) , Sapporo, Hokkaido , 2019/07/18
  • Int'l Joint Research
• [Presentation] Intracellular responses of Coccomyxa sp. during culture in plasma-treated nutrient solution (2019)
  • Author(s): Takumi Kato, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: "The 12th Asian-European International Conference on Plasma Surface Engineering (AEPSE 2019)" , Jeju-do, Korea , 2019/09/02
  • Int'l Joint Research
• [Presentation] Intracellular response of HeLa cells treated by plasma- activated Ringer's lactate solution (2019)
  • Author(s): Shogo Maeda, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine, Fumitaka Kikkawa, Masaaki Mizuno and Masaru Hori
  • Organizer: "The 12th Asian-European International Conference on Plasma Surface Engineering (AEPSE 2019)" , Jeju-do, Korea , 2019/09/02
  • Int'l Joint Research
• [Presentation] Yield improvement of rice plants with cold plasma treatment in paddy field during cultivation. (2019)
  • Author(s): H. Hashizume, H. Kitano, G. Yuasa, S. Tohno, H. Tanaka, K. Ishikawa, S. Matsumoto, H. Sakakibara, S. Nikawa, M. Maeshima, M. Mizuno, and M. Hori
  • Organizer: "The 12th Asian-European International Conference on Plasma Surface Engineering (AEPSE 2019)" , Jeju-do, Korea , 2019/09/02
  • Int'l Joint Research
• [Presentation] Modification of lipid metabolism in Coccomyxa sp. treated by non-equilibrium atmosphere pressure plasma (2019)
  • Author(s): Takumi Kato, Kenji Ishikawa, Hiroshi Hashizume, Hiromasa Tanaka, Takayoshi Tsutsumi, Hiroki Kondo, Makoto Sekine, and Masaru Hori
  • Organizer: MATERIALS RESEARCH MEETING 2019 , Yokohama, Japan , 2019/12/13
  • Int'l Joint Research
• [Presentation] Non-thermal plasma as a powerful tool to synthesize bio-effective materials in solutions (2019)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Nobuhisa Yoshikawa, Hiroaki Kajiyama, Fumitaka Kikkawa, and Masaru Hori
  • Organizer: MATERIALS RESEARCH MEETING 2019 , Yokohama, Japan , 2019/12/11
  • Int'l Joint Research
• [Presentation] 低温プラズマ医療科学の最前線 (2019)
  • Author(s): 田中 宏昌、堀 勝
  • Organizer: 核融合科学研究所と低温プラズマ科学研究センター連携・協力に関する協定書調印式・記念講演会 , 名古屋大学 減災連携研究センター1 階 減災ホール , 2019/10/4
  • Invited
• [Presentation] 低温プラズマの大気圧下での発生・発生源から生体への活性種輸送 (2019)
  • Author(s): 堀 勝、石川健治、田中 宏昌、水野 正明、吉川 史隆、豊國 伸哉
  • Organizer: 第72回日本酸化ストレス学会学術集会 , 北海道立道民活動センター かでる２・７ , 2019/6/27
  • Invited
• [Presentation] プラズマ活性リンゲル液処理による細胞応答と抗腫瘍効果物質の解明 (2019)
  • Author(s): 須田雄貴, 石川健治, 田中宏昌, 橋爪博司, 吉川史隆, 水野正明, 堀勝
  • Organizer: 第80回応用物理学会秋季学術講演会, 北海道大学 札幌キャンパス, 2019年9月18日-21日
• [Presentation] プラズマ活性乳酸リンゲル液（PAL）で培養したHeLa細胞の細胞代謝解析 (2019)
  • Author(s): 前田 昌吾、石川 健治、田中 宏昌、中村 香江、橋爪 博司、吉川 史隆、水野 正明、堀 勝
  • Organizer: 第80回応用物理学会秋季学術講演会, 北海道大学札幌キャンパス, 2019年9月18日-21日
• [Presentation] Mechanism of Aqueous Reactions in Non-equilibrium Atmospheric Pressure Plasma-activated Lactec Solutions (2019)
  • Author(s): Yang Liu、Kenji Ishikawa、Hiroshi Hashizumi、Hiromasa Tanaka、Takayoshi Tsutsumi、Hiroki Kondo、Makoto Sekine、Masaru Hori
  • Organizer: 2019年 第80回応用物理学会秋季学術講演会 , 北海道大学札幌キャンパス , 2019/9/20
• [Presentation] プラズマ活性化乳酸リンゲル点滴 (PAL) によるイネ幼苗の成長促進効果 (2019)
  • Author(s): 橋爪 博司、北野 英己、水野 寛子、湯浅 元気、東野 里江、田中 宏昌、石川 健治、松本 省吾、榊原 均、仁川 進、前島 正義、水野 正明、堀 勝
  • Organizer: 2019年 第80回応用物理学会秋季学術講演会 , 北海道大学札幌キャンパス , 2019/9/20
• [Presentation] プラズマ処理によるイチゴ果実中の抗酸化物質量増加 (2019)
  • Author(s): 橋爪 博司、坪田 憲紀、松本 省吾、伊藤 昌文、湯浅 元気、東野 里江、田中 宏昌、石川 健治、北野 英己、榊原 均、仁川 進、前島 正義、水野 正明、大熊 隆之、堀 勝
  • Organizer: 2019年 第80回応用物理学会秋季学術講演会 , 北海道大学札幌キャンパス , 2019/9/20
• [Presentation] イネ圃場における定期的な低温プラズマ処理による生育や収穫に対する検討 (II) (2019)
  • Author(s): 橋爪 博司、北野 英己、水野 寛子、湯浅 元気、東野 里江、田中 宏昌、石川 健治、松本 省吾、榊原 均、仁川 進、前島 正義、水野 正明、堀 勝
  • Organizer: 2019年 第80回応用物理学会秋季学術講演会 , 北海道大学札幌キャンパス , 2019/9/20
• [Presentation] プラズマ誘起有機酸の前駆体が拓く超バイオ機能 (2019)
  • Author(s): 石川 健治、橋爪 博司、田中 宏昌、吉武 淳、柴田 貴広、小鹿 一、伊藤 昌文、古閑 一憲、白谷 正治、豊國 伸哉、吉川 史隆、水野 正明、堀 勝
  • Organizer: 2019年 第80回応用物理学会秋季学術講演会 , 北海道大学札幌キャンパス , 2019/9/19
• [Presentation] 非平衡大気圧Ar プラズマ源における放電形状の経時変化および水素ラジカル密度空間分布計測 (2019)
  • Author(s): 勝野 楓、堤 隆嘉、石川 健治、竹田 圭吾、橋爪 博司、田中 宏昌、近藤 博基、関根 誠、堀 勝
  • Organizer: 2019年 第80回応用物理学会秋季学術講演会 , 北海道大学札幌キャンパス , 2019/9/20
• [Presentation] Effectiveness and Safety of Plasma Activated Medium (2018)
  • Author(s): Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Shinya Toyokuni, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
  • Organizer: A Meeting of the Materials Research Society
  • Int'l Joint Research / Invited
• [Presentation] Similarities and Differences in Gene Transcription in Plasma-activated Medium Treated and Plasma-activated Ringer’s Lactate Solution Treated Glioblastomas (2018)
  • Author(s): H. Tanaka, M. Mizuno, K. Ishikawa, K. Takeda, H. Hashizume, H. Kondo, M. Sekine, K. Nakamura, H. Kajiyama, S. Akiyama, S. Maruyama, S. Toyokuni, F. Kikkawa, M. Hori
  • Organizer: ICPM7
  • Int'l Joint Research
• [Presentation] プラズマ活性溶液の合成・分析の現状と今後の展望 (2018)
  • Author(s): 田中 宏昌、堀 勝
  • Organizer: 学振136委員会 第138回研究会 『プラズマ誘起による液中化学反応技術の現状と将来展望」
  • Invited
• [Presentation] 様々なプラズマ活性溶液による抗腫瘍効果とその細胞内分子機構 (2018)
  • Author(s): 田中 宏昌、黒川 幸宏、水野 正明、石川 健治、竹田 圭吾、橋爪 博司、中村 香江、梶山 広明、豊國 伸哉、吉川 史隆、堀 勝
  • Organizer: 第79回応用物理学会秋季学術講演会
• [Book] Plasma Cancer Therapy (2020)
  • Author(s): Hiromasa Tanaka, Mounir Laroussi, Sander Bekeschus, Dayun Yan, Masaru Hori, and Michael Keidar
  • Total Pages: 303
  • Publisher: Springer
  • ISBN: 9783030499662
• [Remarks] 低温プラズマ科学研究センター
  • URL: https://www.plasma.nagoya-u.ac.jp/
• [Patent(Industrial Property Rights)] イネの生産方法 (2020)
  • Inventor(s): 堀勝、田中宏昌、他7名
  • Industrial Property Rights Holder: 堀勝、田中宏昌、他7名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
• [Patent(Industrial Property Rights)] イネの生産方法 (2019)
  • Inventor(s): 堀 勝、橋爪 博司、田中 宏昌、他6名
  • Industrial Property Rights Holder: 堀 勝、橋爪 博司、田中 宏昌、他6名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] イネの生産方法 (2019)
  • Inventor(s): 堀 勝、橋爪 博司、田中 宏昌、他6名
  • Industrial Property Rights Holder: 堀 勝、橋爪 博司、田中 宏昌、他6名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019
• [Patent(Industrial Property Rights)] 腹腔内洗浄溶液の製造方法 (2018)
  • Inventor(s): 吉川 史隆、田中 宏昌、堀 勝、他9名
  • Industrial Property Rights Holder: 吉川 史隆、田中 宏昌、堀 勝、他9名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018
• [Patent(Industrial Property Rights)] 創傷治癒促進溶液の製造方法 (2018)
  • Inventor(s): 吉川 史隆、田中 宏昌、堀 勝、他11名
  • Industrial Property Rights Holder: 吉川 史隆、田中 宏昌、堀 勝、他11名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018