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Quantification of bacterial chemotaxis by use of observation of collective motion of cells and mathematical model based on individual behavior

Research Project

Project/Area Number 18K03950
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 19010:Fluid engineering-related
Research InstitutionTottori University

Principal Investigator

GOTO Tomonobu  鳥取大学, 工学研究科, 教授 (00260654)

Co-Investigator(Kenkyū-buntansha) 中井 唱  鳥取大学, 工学研究科, 准教授 (80452548)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords走化性 / 細菌 / 集積 / 誘引物質 / 数理モデル / 誘因物質 / 単毛性細菌 / べん毛 / 方向転換 / 定量化 / 個体運動
Outline of Final Research Achievements

Accumulation of bacterial cells around several amino acids and swimming motion of individual cell were observed. Two strains of cells were used: One of them is Salmonella typhimurium, SJW1103, which is peritrichous, and the other is Vibrio alginolyticus, YM4, monotrichous. 1) Salmonella cells accumulate more around aspartic acid than around L-serine. 2) Vibrio cells accumulate more around L-serine than around glycine, leucine, alanine. 3) Chemotaxis had been thought to be caused by suppression of tumbles. However, we found that biased reorientation is another cause of chemotaxis. In Salmonella cells, chemotaxis occurs mainly due to the biased reorientation. 4) Numerical simulation including mathematical model for individual behavior of each cell revealed that the difference in individual motion between Salmonella (peritrichous) and Vibrio (monotrichous) produces the difference in number density distribution of cells around an attractant chemical.

Academic Significance and Societal Importance of the Research Achievements

誘引物質に対する細菌の集積速度や誘引物質まわり細菌分布を予測するためには,個々の細菌の運動から集団運動としての走化性の強さを同定する必要がある.このことは,狭い環境下では,細菌の増殖と合わせて生じるぬめりなどの形成の定量評価に寄与するであろうし,広い環境下では,食物連鎖の中の細菌の役割を明らかにすることに繋がる.また,有用細菌の培養条件の設定や,最近試みられている集団運動の制御にも寄与するものと考えられる.
ここで得られた研究成果は,走化性の強さを同定するための方法の開発の基礎となると同時に,走化性の強さを示すデータの一部となる.

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (21 results)

All 2022 2021 2020 2019 2018 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (17 results) (of which Int'l Joint Research: 7 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Difference in chemotaxis of <i>Salmonella</i> cells between serine and aspartic acid2021

    • Author(s)
      NAKAI Tonau, HIGASHIDA Yasutoshi, NAKAMURA Fumiya、GOTO Tomonobu
    • Journal Title

      Journal of Biomechanical Science and Engineering

      Volume: 16 Issue: 3 Pages: 21-00074-21-00074

    • DOI

      10.1299/jbse.21-00074

    • NAID

      130008088411

    • ISSN
      1880-9863
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biased reorientation in the chemotaxis of peritrichous bacteria Salmonella enterica serovar Typhimurium2021

    • Author(s)
      Nakai Tonau, Ando Taishi, Goto Tomonobu
    • Journal Title

      Biophysical Journal

      Volume: 120 Issue: 13 Pages: 2623-2630

    • DOI

      10.1016/j.bpj.2021.04.033

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimation of chemotaxis intensity of <i>Salmonella</i> cells by the capillary assay and biased random walk simulation2019

    • Author(s)
      Tonau Nakai, Junpei Yoshino, Tomonobu Goto
    • Journal Title

      Journal of Aero Aqua Bio-mechanisms

      Volume: 8 Issue: 1 Pages: 63-68

    • DOI

      10.5226/jabmech.8.63

    • NAID

      130007676841

    • ISSN
      2185-1522
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] BIASED REORIENTATION IN THE CHEMOTAXIS OF PERITRICHOUS BACTERIA2022

    • Author(s)
      Tonau Nakai, Tomonobu Goto
    • Organizer
      Biological fluids & flows - A conference to 80th birthday of Prof. Tim Pedley, FRS
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Difference in the chemotaxis between monotrichous and peritrichous bacteria by a numerical simulation and observation2021

    • Author(s)
      Yuta Kunimasa, Tonau Nakai, Tomonobu Goto
    • Organizer
      The 11th Asian-Pacific Conference on Biomechanics (AP Biomech 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biased Re-orientation in chemotaxis of peritrichous bacteria2021

    • Author(s)
      Tonau Nakai, Taishi Ando, Tomonobu Goto
    • Organizer
      25th International Congress of Theoretical and Applied Mechanics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細菌の走化性強さの観測と数理モデルによる評価2021

    • Author(s)
      中井 唱,國政 裕太,後藤 知伸
    • Organizer
      第60回 日本生体医工学会大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ピエゾ駆動レンズで取得した映像のOpenCVを用いた画像処理による細菌の三次元遊泳計測2021

    • Author(s)
      中嶋 貴也,中井 唱,後藤 知伸
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] 数理モデルを用いた細菌の走化性強さの推定2020

    • Author(s)
      中井 唱,後藤 知伸
    • Organizer
      日本生物物理学会 第58回年会 シンポジウム 「もっと面白くなる細菌べん毛研究~残された宿題への挑戦~」
    • Related Report
      2020 Research-status Report
  • [Presentation] 観察に基づくバイアス付きランダムウォークモデルによる単毛性細菌及び周毛性細菌の走化性シミュレーション2020

    • Author(s)
      國政裕太,中井 唱,後藤 知伸
    • Organizer
      日本機械学会 第31回バイオフロンティア講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] Difference in Chemotaxis of Salmonella Cells between Different Amino Acids2019

    • Author(s)
      Tonau Nakai, Taishi Ando, Fumiya Nakamura, Yasutoshi Higashida, Tomonobu Goto
    • Organizer
      The 10th Asian-Pacific Conference on Biomechanics, Taipei
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Estimation of Chemotaxis Intensity in Bacteria by Using a Mathematical Model2019

    • Author(s)
      Tonau Nakai, Yasutoshi Higashida, Fumiya Nakamura, Taishi Ando, Tomonobu Goto
    • Organizer
      Mathematical Methods in Biofluid Mechanics (a RIMS Satellite Seminar in 2019), Awaji
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] サルモネラ菌の走化性の誘引物質による差異2019

    • Author(s)
      中井 唱,後藤 知伸
    • Organizer
      第40回エアロ・アクアバイオメカニズム学会定例講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] サルモネラ菌の走化性の誘引物質による差異2019

    • Author(s)
      中村 文哉,東田 康稔,安藤 大師,中井 唱,後藤 知伸
    • Organizer
      日本機械学会第30回バイオフロンティア講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] セリンとアスパラギン酸に対するサルモネラ菌の走化性の差異2019

    • Author(s)
      中井唱,東田康稔,中村文哉,安藤大師,後藤知伸
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] サルモネラ菌の走化性強さの観察と数理モデルによる推定2018

    • Author(s)
      中井唱,吉野純平,安藤大師,後藤知伸
    • Organizer
      日本機械学会 ロボティクス・メカトロニクス講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] A scale for quantitative measure on strength of bacterial chemotaxis2018

    • Author(s)
      Tomonobu Goto, Mami Kajitani, Junpei Yoshino, Tonau Nakai
    • Organizer
      8th World Congress of Biomechanics
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Chemotaxis measurement for a single bacterial cell and cell group2018

    • Author(s)
      Tonau Nakai, Junpei Yoshino, Taishi Ando, Tomonobu Goto
    • Organizer
      The Seventh International Symposium on Aero Aqua Bio-mechanisms
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 誘引物質まわりの細菌単体の挙動計測2018

    • Author(s)
      安藤大師,中井唱,後藤知伸
    • Organizer
      日本機械学会年次大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 細菌単体の挙動計測に基づく走化性効果の再考2018

    • Author(s)
      安藤大師,中井唱,後藤知伸
    • Organizer
      第39回エアロ・アクアバイオメカニズム学会定例講演会
    • Related Report
      2018 Research-status Report
  • [Remarks] 生体システム解析学研究室

    • URL

      http://www.damp.tottori-u.ac.jp/~lab5/index.html

    • Related Report
      2021 Annual Research Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

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