Shortening of measurement time for in vivo digital EPR spectrometer

• Project/Area Number: 18K04164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 21030:Measurement engineering-related
• Research Institution: Osaka University
• Principal Investigator: Hideo Akaba 大阪大学, 基礎工学研究科, 准教授 (00552077)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 電子常磁性共鳴 / 電子スピン共鳴 / デジタルEPR分光器 / In vivo 生体イメージング / EPRイメージング / 自動整合回路 / EPRデジタル分光器 / EPR / 生体計測 / デジタル信号処理 / 高速磁場掃引

Outline of Final Research Achievements

We have developed the technology needed to shorten spectral acquisition with a digital electron paramagnetic resonance (EPR) spectrometer for biological applications. Based on the results of electromagnetic simulation, a compact arrayed magnet without a yoke structure for EPR was fabricated. Low inductance gradient coils and sweep coil suitable for high speed control of the magnetic field have been successfully attached to the EPR magnet. We have also developed an automatic amplitude / phase control feedback system that enables high-speed magnetic field scanning, and succeeded in acquiring the EPR spectrum by magnetic field scanning at approximately 10kHz to 60kHz. As a result, it has become possible to acquire 3D spatial imaging of free radical molecules with 181 projections in about 4 seconds.

Academic Significance and Societal Importance of the Research Achievements

本研究では、デジタルEPR分光器の高速化に必要な磁気共鳴用マグネットを可搬可能な小型軽量に製作する技術や新しい磁場掃引制御方法を提案し実証した。今後、開発した高速なデジタルEPR分光器を用いた可搬型フリーラジカル分子イメージングシステムの開発に着手し、多くの生命科学研究者が生体用EPR技術を容易に応用できるようにしたい。

Research Products

• [Journal Article] Design and Fabrication of Compact Arrayed Magnet for Biological EPR Imaging (2020)
  • Author(s): Sato-Akaba Hideo、Okada Yuki、Tsuji Kentaro、Emoto Miho C.、Fujii Hirotada G.
  • Journal Title: Applied Magnetic Resonance Volume: － Issue: 8 Pages: 1-13
  • DOI: 10.1007/s00723-020-01256-4
  • Peer Reviewed
• [Journal Article] Development of an L-band rapid scan EPR digital console (2019)
  • Author(s): Sato-Akaba Hideo,Tseytlin Mark
  • Journal Title: Journal of Magnetic Resonance Volume: 304 Pages: 42-52
  • DOI: 10.1016/j.jmr.2019.05.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Brain Redox Imaging Using In Vivo Electron Paramagnetic Resonance Imaging and Nitroxide Imaging Probes (2019)
  • Author(s): Fujii Hirotada、Emoto Miho、Sato-Akaba Hideo
  • Journal Title: Magnetochemistry Volume: 5 Issue: 1 Pages: 11-11
  • DOI: 10.3390/magnetochemistry5010011
  • Peer Reviewed
• [Journal Article] Non-invasive mapping of glutathione levels in mouse brains by in vivo electron paramagnetic resonance (EPR) imaging: Applied to a kindling mouse model (2019)
  • Author(s): Emoto Miho C.、Sato-Akaba Hideo、Matsuoka Yuta、Yamada Ken-ichi、Fujii Hirotada G.
  • Journal Title: Neuroscience Letters Volume: 690 Pages: 6-10
  • DOI: 10.1016/j.neulet.2018.10.001
  • Peer Reviewed
• [Presentation] Lバンド・ラピッドスキャンEPRデジタル分光器の開発 (2019)
  • Author(s): 大槻智也, 辻健太郎, 赤羽英夫
  • Organizer: 第58回電子スピンサイエンス学会年会
• [Presentation] EPR生体イメージング用静磁場マグネットの設計 (2019)
  • Author(s): 岡田侑樹, 辻健太郎, 赤羽英夫
  • Organizer: 第58回電子スピンサイエンス学会年会
• [Presentation] 電子スピン共鳴用L-band共振器の自動整合制御機構の開発 (2018)
  • Author(s): 濱垣 研太、宮戸 祐治、赤羽 英夫
  • Organizer: 第79回応用物理学会秋季学術講演会
• [Presentation] L-band CW-EPRにおける送受信干渉抑制機構の解析 (2018)
  • Author(s): 濱垣 研太、宮戸 祐治、赤羽 英夫
  • Organizer: 第57回電子スピンサイエンス学会年会