Hybrid transfection using a combination of nanosecond shock wave and pulse electric field with piezoelectric loading device
| Project/Area Number |
18K04271
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Review Section |
Basic Section 21060:Electron device and electronic equipment-related
|
|---|
| Research Institution | Kanazawa Institute of Technology |
|---|
Principal Investigator |
Aizawa Koji 金沢工業大学, 工学部, 教授 (40222450)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
小木 美恵子 金沢工業大学, 基礎教育部, 教授 (50410288)
|
|---|
| Project Period (FY) |
2018-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | パルス電界 / 衝撃波 / 外来物質導入 / 圧電体 / パルスレーザ / ナノ秒 / 圧電 / トランスフェクション |
|---|
| Outline of Final Research Achievements |
In this study, we developed a general-purpose system for introducing foreign substances into human cells. A developed system (hybrid transfection system) is composed of a combination of shock wave and pulsed electric field using a commercially available electroporation cuvette introducing a piezoelectric material. We also applied this system to yeast (S. cerevisiae) and experimentally investigated its effect.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究で行ったヒト細胞へのナノ秒衝撃波照射実験およびパルス電界印加実験の成果は外来物質導入に関する新たな知見をいくつか与えている．また圧電体導入による印加電界の増強効果は従来法にはない現象で導入効率向上につながる可能性がある．本研究で構築したシステムは，市販の細胞培養容器を使っているため汎用性があり，酵母に対する効果を実験的に確かめるなど，ヒト細胞以外にも応用できることを示した点で意義がある．
|
Report
(4 results)
Research Products
(13 results)
