High-performance biomaterial with essential metal ions to supply to in the living body

• Project/Area Number: 18K04734
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 26030:Composite materials and interfaces-related
• Research Institution: Tokyo Denki University
• Principal Investigator: Hirakuri Kenji 東京電機大学, 工学部, 教授 (60225505)
• Co-Investigator(Kenkyū-buntansha): 馬目 佳信 東京慈恵会医科大学, 医学部, 教授 (30219539)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Zn-DLCコーティング / Zn溶出 / 骨代謝 / 深さ方向元素分析 / 骨折治療 / DLCコーティング / 生体必須元素 / 動物実験 / 生体親和性 / 骨芽細胞 / Zn徐放

Outline of Final Research Achievements

This study proposes that osteogenesis ability of zinc (Zn)-containing diamond-like carbon (Zn-DLC) splint was assessed in vivo experiments using a mouse model. The results of the in vivo tests using mice demonstrated that the use of Zn-DLC splints tend to stimulate the bone to grow thick. It was also shown that Zn-DLC can elute Zn even in different biological environments and that the elution amount can be the highest in an in vivo environment. These results indicate that Zn-DLC may improve upon the therapeutic effect of conventional implants with the eluted Zn in healing fractures.

Academic Significance and Societal Importance of the Research Achievements

本研究では、DLC特有の抗血栓性、化学的安定性、低摩擦、高硬度などの静的特性に加え、生体細胞の活性化に重要な役割を演じる金属元素である亜鉛（Zn）をドープすることで、生体活性元素を含有したDLCによる表面コーティング技術のバイオマテリアルへの展開を目的とした。この研究結果を「生体内におけるZn溶出型DLC膜の骨形成促進効果」、「Z生体材料としてのZn-DLCの深さ方向におけるZn溶出特性」、「骨折治療に有効な生体材料であるZn-DLC」とした発表を行い、研究成果の社会還元に寄与した（研究発表参照）。

Research Products

• [Journal Article] Antibacterial property of F doped DLC film with plasma treatment (2020)
  • Author(s): S. Onodera, S. Fujii, H. Moriguchi, M.Tsujioka, K. Hirakuri
  • Journal Title: Diamond and Related Materials, Volume: 107 Pages: 1-7
  • DOI: 10.1016/j.diamond.2020.107835
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of sliding properties and durability of DLC coating for medical devices (2020)
  • Author(s): K. Sakurai, M. Hiratsuka, H. Nakamori, K. Nakamori, K. Hirakuri
  • Journal Title: Diamond and Related Materials Volume: 96 Pages: 97-103
  • DOI: 10.1016/j.diamond.2019.03.021
  • Peer Reviewed / Open Access
• [Journal Article] DLCのバイオマテリアルへの応用 (2019)
  • Author(s): 平栗建二、小野寺修、齋藤一拓
  • Journal Title: メカニカル・サーフェス・テック Volume: 12 Pages: 31-33
• [Journal Article] Coupling of bone resorption and formation by RANKL reverse signalling. (2018)
  • Author(s): Ikebuchi Y, Aoki S, Honma M, Hayashi M, Sugamori Y, Khan M, Kariya Y, Kato G, Tabata Y, Penninger JM, Udagawa N, Aoki K, Suzuki H
  • Journal Title: Nature Volume: 561 Issue: 7722 Pages: 195-200
  • DOI: 10.1038/s41586-018-0482-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] DLC coting with UV protection and antibacterial property (2020)
  • Author(s): Shu Onodera, Shinya Fujii, Hideki Moriguchi, Masanori Tsujioka and Kenji Hirakuri
  • Organizer: International Conference on Diamond and Carbon Materials
  • Int'l Joint Research
• [Presentation] Effect of plasma frequency for nitrogen doped DLC films] (2020)
  • Author(s): T. Kaiho, A. Homma, K. Hirakuri, H. Sumikura, Y. Ohgoe
  • Organizer: ICDCM 2019 (30th International Conference on Diamond and Carbon Material）
  • Int'l Joint Research
• [Presentation] Anticorrosion and durability of DLC coatings on copper alloy (2020)
  • Author(s): Mao Kaneko, Kazushige Namiki, Shinya Fujii, Hideki Moriguchi, Masanori Tsujioka and Kenji Hirakuri
  • Organizer: ICDCM 2019 (30th International Conference on Diamond and Carbon Material）
  • Int'l Joint Research
• [Presentation] Evaluation of Zn elution from Zn-DLC as biomaterial (2020)
  • Author(s): Kazuhiro Saito, Yasuharu Ohgoe, Keisuke Sato, Masanori Hiratsuka, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
  • Organizer: ICDCM 2019 (30th International Conference on Diamond and Carbon Material）
  • Int'l Joint Research
• [Presentation] Investigation of nitrogen-containing DLC thin films for biological response by neutron reflectometry (2020)
  • Author(s): N. Tatsumi, Y. Ohgoe, N. Miyata, T. Miyazaki, T. Yaguchi, K. Hirakuri, A. Homma, H. Sumikura
  • Organizer: ICDCM 2019 (30th International Conference on Diamond and Carbon Material）
  • Int'l Joint Research
• [Presentation] Improvement of wear property or acid and sterilization treatment for medical device by DLC coating (2020)
  • Author(s): Kengo Sakurai, Kyoichi Terai, Hideki Nakamori, Masanori Hiratsuka, Kazushige Namiki, Kenji Hirakuri
  • Organizer: International Conference on Diamond and Carbon Materials
  • Int'l Joint Research
• [Presentation] Evaluation of fracture treatment of Zn-DLC for biomaterial application (2020)
  • Author(s): Shunta Kidera, Kouki Fujioka, Yasuharu Ohgoe, Keisuke Sato, Masanori Hiratsuka, Hitomi Tsuboi, Hideki Nakamori, Hideki Masuda, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
  • Organizer: International Conference on Material and Chemical Engineering
  • Int'l Joint Research
• [Presentation] 生体内におけるZn-DLCのZn溶出評価 (2020)
  • Author(s): 齋藤一拓, 木寺俊太, 藤岡宏樹, 馬目佳信, 平塚傑工, 本田宏志, 大越康晴, 佐藤慶介,平栗健二
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] 生体材料としてのZn-DLCの深さ方向におけるZn溶出特性 (2020)
  • Author(s): 齋藤一拓、藤岡宏樹、馬目佳信、平塚傑工、本田宏志、大越康晴、平栗健二
  • Organizer: 第80回応用物理学会秋季学術講演会
• [Presentation] 骨折治療に有効な生体材料であるZn-DL (2020)
  • Author(s): 齋藤一拓、馬目佳信、平塚傑工、本田宏志、大越康晴、平栗健二
  • Organizer: 第33回ダイヤモンドシンポジウム
• [Presentation] Keynote speech (2019)
  • Author(s): Kenji Hirakuri
  • Organizer: Biomedical materials, advances in medical imaging technology, and cell therapy, Japan-Taiwan Medical Sciences Frontiers Forum
  • Int'l Joint Research / Invited
• [Presentation] Evaluation of Zn elution from Zn-DLC as biomaterial (2019)
  • Author(s): Kazuhiro Saito, Yasuharu Ohgoe, Keisuke Sato, Masanori Hiratsuka, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
  • Organizer: International Conference on Diamond and Carbon Materials
  • Int'l Joint Research
• [Presentation] Zn-DLC for effective treatment of fracture (2019)
  • Author(s): Kazuhiro Saito, Yasuharu Ohgoe, Masanori Hiratsuka, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakur
  • Organizer: International Symposium on Engineering and Natural Sciences
  • Int'l Joint Research
• [Presentation] 生体材料としてのZn-DLCの深さ方向におけるZn溶出特性 (2019)
  • Author(s): 齋藤一拓、馬目佳信、平塚傑工、本田宏志、大越康晴、平栗健二
  • Organizer: 第80回応用物理学会秋季学術講演会
• [Presentation] 折治療に有効な生体材料であるZn-DLC (2019)
  • Author(s): 4.齋藤一拓、藤岡宏樹、馬目佳信、平塚傑工、本田宏志、大越康晴、平栗健二
  • Organizer: 第33回ダイヤモンドシンポジウム
• [Presentation] 生体内におけるZn-DLCのZn溶出評価 (2019)
  • Author(s): 4.齋藤一拓, 木寺俊太, 藤岡宏樹, 馬目佳信, 平塚傑工, 本田宏志, 大越康晴, 佐藤慶介,平栗健二
  • Organizer: 第66回応用物理学会春季学術講演会
• [Presentation] Time course of Zn release from Zn-containing DLC (2018)
  • Author(s): 1.Shunta Kidera, Kouki Fujioka, Yasuharu Ohgoe, Keisuke Sato, Masanori Hiratsuka, Hitomi Tsuboi, Hideki Nakamori, Hideki Masuda, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
  • Organizer: International Conference on Diamond and Carbon Materials,
  • Int'l Joint Research
• [Presentation] Evaluation of fracture treatment of Zn-DLC for biomaterial application (2018)
  • Author(s): 2.Shunta Kidera, Kouki Fujioka, Yasuharu Ohgoe, Keisuke Sato, Masanori Hiratsuka, Hitomi Tsuboi, Hideki Nakamori, Hideki Masuda, Hiroshi Honda, Yoshinobu Manome, Kenji Hirakuri
  • Organizer: International Conference on Material and Chemical Engineering
  • Int'l Joint Research
• [Presentation] Effect of Zn-release diamond-like carbon films on bone formation in vivo (2018)
  • Author(s): 3.Kidera Shunta, Katouno Junichi, Fujioka Kouki, Ohgoe Yasuharu, Sato Keisuke, Hiratsuka Masanori, Tsuboi Hitomi, Nakamori Hideki, Masuda Hideki, Honda Hiroshi, Manome Yoshinobu, Hirakuri Kenji
  • Organizer: 第57回日本生体医工学会大会
• [Book] 医用工学の基礎 (2020)
  • Author(s): 土肥健純、平栗健二
  • Total Pages: 240
  • Publisher: 東京電機大学出版
  • ISBN: 9784501333201