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Nanobio antifungal agent for fungal pandemics

Research Project

Project/Area Number 18K04842
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionTohoku University

Principal Investigator

NAKAZAWA HIKARU  東北大学, 工学研究科, 准教授 (40584991)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsタンパク質工学 / 無機ナノ粒子 / 酵素 / 表面提示 / キチナーゼ活性 / 抗菌活性 / クラスター化 / インターフェイス / 銀ナノ粒子 / キチナーゼ / 高密度提示 / 高活性化 / 安定化 / 酵素ライブラリー / 抗真菌剤 / ワンポット合成
Outline of Final Research Achievements

Japanese mold (Candida aureas) has become poisonous overseas, causing the first mycotic pandemic (global epidemic) in Europe, the United States and Asia.The development of antifungal agents with a new mechanism of action that is difficult to have resistance is expected.In this study, we have developed a nanobio antifungal agent that exhibits antifungal activity by using the natural antifungal enzyme chitinase on the surface of silver nanoparticles that have high antifungal activity and are less likely to develop resistance.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、全く異なる安定性や機能、および反応環境を有する無機ナノ粒子とタンパク質という2種の物質の融合研究である。両方の性質を十分に活かす最適条件と構造を開発することで波及効果を見込む。
社会的意義については、耐性を持ちやすい真菌症のパンデミックに対抗する手段として新たな作用機序の抗真菌剤を開発し社会に貢献する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (21 results)

All 2021 2020 2019 2018

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Association behavior and control of the quality of cancer therapeutic bispecific diabodies expressed in Escherichia coli2020

    • Author(s)
      Hikaru Nakazawa, Tomoko Onodera-Sugano, Aruto Sugiyama, Yoshikazu Tanaka, Takamitsu Hattori, Teppei Niide, Hiromi Ogata, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      Biochemical Engineering Journal

      Volume: 160 Pages: 1-9

    • DOI

      10.1016/j.bej.2020.107636

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chemically Crosslinked Bispecific Antibodies for Cancer Therapy: Breaking from the Structural Restrictions of the Genetic Fusion Approach2020

    • Author(s)
      Ueda Asami, Umetsu Mitsuo, Nakanishi Takeshi, Hashikami Kentaro, Nakazawa Hikaru, Hattori Shuhei, Asano Ryutaro, Kumagai Izumi
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 21 Issue: 3 Pages: 711-714

    • DOI

      10.3390/ijms21030711

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nanoparticle assisted remodeling of proteotoxic SOD1 mutants alters the bio-interface of the functional interaction of microtubules and kinesin motors2019

    • Author(s)
      Kim Kyongwan, Subramaniyam, Selvaraj, Galaleldeen Ahmad, Nakazawa Hikaru, Umetsu Mitsuo, Winfried Teizer, Bhattacharyya Sanjib
    • Journal Title

      ACS Appl. Bio Mater.

      Volume: 2 Issue: 10 Pages: 4121-4128

    • DOI

      10.1021/acsabm.9b00501

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of Indium Tin Oxide Nanoparticle-Binding Peptides via Phage Display and Biopanning Under Various Buffer Conditions2019

    • Author(s)
      Hikaru Nakazawa, Mitsuo Umetsu, Hirose Tatsuya, Takamitsu Hattori, Izumi Kumagai
    • Journal Title

      Protein and peptide letters

      Volume: 1 Issue: 6 Pages: 27-37

    • DOI

      10.2174/0929866526666191113151934

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Complementary Design for Pairing between Two Types of Nanoparticles Mediated by a Bispecific Antibody: Bottom-Up Formation of Porous Materials from Nanoparticles2019

    • Author(s)
      Niide Teppei、Manabe Noriyoshi、Nakazawa Hikaru、Akagi Kazuto、Hattori Takamitsu、Kumagai Izumi、Umetsu Mitsuo
    • Journal Title

      Langmuir

      Volume: 35 Issue: 8 Pages: 3067-3076

    • DOI

      10.1021/acs.langmuir.8b03687

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Impact in stability during sequential CDR grafting to construct camelid VHH antibodies against zinc oxide and gold2018

    • Author(s)
      Saito Ryota、Saito Yutaro、Nakazawa Hikaru、Hattori Takamitsu、Kumagai Izumi、Umetsu Mitsuo、Makabe Koki
    • Journal Title

      The Journal of Biochemistry

      Volume: 164 Issue: 1 Pages: 21-25

    • DOI

      10.1093/jb/mvy016

    • NAID

      40021625279

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins2018

    • Author(s)
      Saito Yutaka、Oikawa Misaki、Nakazawa Hikaru、Niide Teppei、Kameda Tomoshi、Tsuda Koji、Umetsu Mitsuo
    • Journal Title

      ACS Synthetic Biology

      Volume: 7 Issue: 9 Pages: 2014-2022

    • DOI

      10.1021/acssynbio.8b00155

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] がん細胞選択的遺伝子治療を想定した抗体-膜貫通ペプチド酵素的架橋設計2021

    • Author(s)
      中澤 光, 安藤 優, 三浦 大輔, 梅津 光央
    • Organizer
      農芸化学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細胞選択的遺伝子治療を想定した抗体-膜貫通ペプチド酵素的架橋設計2020

    • Author(s)
      中澤 光, 安藤 優, 三浦 大輔, 梅津 光央
    • Organizer
      化学工学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] タンパク質の自動設計は可能になるのか?:ドメインライブラリーアプローチと機械学習支援2019

    • Author(s)
      中澤 光
    • Organizer
      日本化学会 バイオ関連化学若手フォーラム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 蛋白質機能を向上するためのドメインライブラリー設計2019

    • Author(s)
      中澤 光
    • Organizer
      日本化学会 東北支部会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 分子認識アプタマー開発における進化分子工学操作の新しい展開2019

    • Author(s)
      中澤 光
    • Organizer
      日本分析化学会第79回分析化学討論会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Dlag Art法を用いた迅速確実なT-cell recruiting抗体スクリーニングプロセス2019

    • Author(s)
      中澤 光, 杉山在生人, 二井手哲平, 浅野竜太郎, 梅津光央
    • Organizer
      第18回蛋白質科学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 太陽光で活性化される光応答型人工セルラーゼ設計2018

    • Author(s)
      Hikaru Nakazawa, Yuri Ishigaki, Teppei Niide, Mitsuo Umetsu
    • Organizer
      生物工学会若手の会
    • Related Report
      2018 Research-status Report
  • [Presentation] 光に応答して活性化するハイブリッド酵素デザイン2018

    • Author(s)
      Hikaru Nakazawa, Yuri Ishigaki, Teppei Niide, Mitsuo Umetsu
    • Organizer
      酵素工学研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] CBH触媒モジュール高発現型 人工セルロソームの設計2018

    • Author(s)
      中澤 光, 石垣友理, 張 鈺爽, 坪井宏和, 幸田明生, 坊垣隆之, 梅津光央
    • Organizer
      生物工学会
    • Related Report
      2018 Research-status Report
  • [Presentation] A design of artificial cellulosome with high expression of CBH catalytic module2018

    • Author(s)
      Hikaru Nakazawa, Yuri Ishigaki, Zhang Yushuang, Hirokazu Tsuboi, Akio Koda, Takayuki Bogaki, Mitsuo Umetsu
    • Organizer
      化学工学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Identification of Indium Tin Oxide Nanoparticle-binding peptides by Using Phage display and Biopanning2018

    • Author(s)
      Hikaru Nakazawa, Tatsuya Hirose, Takamitsu Hattori, Izumi kumagai, Mitsuo Umetsu
    • Organizer
      第174委員会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 生物工学会誌 バイオミディア 酵素はメカ化できるのか?2019

    • Author(s)
      中澤 光
    • Total Pages
      1
    • Publisher
      生物工学会
    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] ダイアボディ型BsAbを発現・分泌するビフィドバクテリウム属細菌2019

    • Inventor(s)
      中澤 光
    • Industrial Property Rights Holder
      中澤 光
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-155792
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] プラスミド一周PCR法を用いたランダム遺伝子プラスミドライブラリー作成方法、それを用いた形質転換体作成方法、および、その方法に使用するキット2018

    • Inventor(s)
      柳沢美貴、中澤光、二井手哲平、服部峰充、梅津光央
    • Industrial Property Rights Holder
      柳沢美貴、中澤光、二井手哲平、服部峰充、梅津光央
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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