| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
A method to identify electron transfer proteins and their reductases required for cytochrome P450s has been developed. Selective protein assembly using subunits of a heterotrimeric proteins as concatenating units enabled to identify reductases that can reduce target electron transfer protein using cell lysates. Furthermore, the selective assembly of the three proteins enabled to identify the redox partner proteins by utilizing the competition between the interaction of electron transfer proteins with cytochrome P450s and that with reductases, which are not detected in free protein solution,
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