• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Discovery of specific redox systems for bacterial cytochrome P450s

Research Project

Project/Area Number 18K04846
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Hirakawa Hidehiko  筑波大学, 生命環境系, 准教授 (90451799)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsシトクロムP450 / 電子伝達システム / スクリーニング / 自己集合
Outline of Final Research Achievements

A method to identify electron transfer proteins and their reductases required for cytochrome P450s has been developed. Selective protein assembly using subunits of a heterotrimeric proteins as concatenating units enabled to identify reductases that can reduce target electron transfer protein using cell lysates. Furthermore, the selective assembly of the three proteins enabled to identify the redox partner proteins by utilizing the competition between the interaction of electron transfer proteins with cytochrome P450s and that with reductases, which are not detected in free protein solution,

Academic Significance and Societal Importance of the Research Achievements

医農薬品及びその代謝物などの合成用触媒としての利用が期待されているシトクロムP450は、触媒活性に2つの補助タンパク質(電子伝達タンパク質、還元酵素)を必要とする。ゲノム解析により、シトクロムP450は生物界に広く存在していることが明らかになっているものの、パートナーとなる補助タンパク質の特定が容易ではないため、その利用は進んでいない。本研究では、選択的な近接化により、シトクロムP450の触媒反応を評価せずにパートナーとなる補助タンパク質を特定する方法論を確立しており、今後のシトクロムP450の利用および研究を大きく発展させるものと期待される。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (5 results)

All 2021 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Artificial Self‐Sufficient Cytochrome P450 Containing Multiple Auxiliary Proteins Demonstrates Improved Monooxygenase Activity2018

    • Author(s)
      Haga Tomoaki、Hirakawa Hidehiko、Nagamune Teruyuki
    • Journal Title

      Biotechnology Journal

      Volume: 13 Issue: 12 Pages: 1800088-1800088

    • DOI

      10.1002/biot.201800088

    • NAID

      120007127987

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Artificial proximity of electron transfer protein to cytochrome P450 opens a treasure chest2021

    • Author(s)
      Hidehiko Hirakawa
    • Organizer
      Pacifichem 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 人為的な近接化を利用したシトクロムP450camープチダレドキシン間相互作用の検出2019

    • Author(s)
      齋藤 航汰、 平川 秀彦、市川 創作
    • Organizer
      酵素工学研究会第82回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 複数の酸化還元ドメインを有する人工自己充足型シトクロムP450における電子伝達プロセスの解析2018

    • Author(s)
      平川秀彦
    • Organizer
      第31回生物無機化学夏季セミナー
    • Related Report
      2018 Research-status Report
  • [Presentation] What is happening in an artificial self-sufficient cytochrome P450?2018

    • Author(s)
      Hidehiko Hirakawa
    • Organizer
      The 15th Japan-China-Korea Joint Symposium on Enzyme Engineering
    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi