| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
When TALE was prepared by deliberately introducing a mismatch at a specific position, the difference in one base of DNA was clearly distinguished, and the dissociation constant changed dramatically. TALEN produced by the mismatch method selectively cleaved the target sequence, and did not cleave non-target sequences that differed by one base. This result means that the TALE prepared by the mismatch method has higher sequence specificity and accuracy than the TALE prepared by the conventional method.
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