| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We developed a new microfluidic device that realizes efficient electrofusion of nanoscale vesicles for the detection and analysis of vesicles derived from living cells. This device is equipped with silicon electrodes bonded to a glass substrate for electrofusion and PDMS microwells for trapping vesicles. GUVs (giant unilamellar vesicles) were prepared from phospholipids using the water-in-oil (W/O) emulsion transfer method, and then two different sizes of vesicles were prepared using a metal mesh filter with a high aperture ratio. After introducing vesicles into the fusion device, voltage pulse was applied under observation with a fluorescence microscope. We successfully performed trapping in some of the microwells and electrofusion of two sizes of vesicles.
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