| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
This research aimed to utilize Raman optical activity (ROA) spectroscopy for the measurement of the three-dimensional (3D) structure of the reaction intermediates in photoreceptor proteins. We chose the photoreceptors, such as light-driven ion pumps, of micro-organisms and planned to measure ROA of their initial photointermediates trapped at low temperature. As the important step for this ROA measurement, we could measured the low-temperature Raman spectra of the initial intermediates of various photoreceptors and studied their early-time structural changes in the photoreactions. We found a couple of photoreceptors, to which the ROA spectroscopy can be applied, and worked on the development of the low-temperature ROA spectrometer.
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