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Raman optical activity measurement of reaction intermediate of photoreceptor proteins and its application

Research Project

Project/Area Number 18K05037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 32010:Fundamental physical chemistry-related
Research InstitutionSaga University

Principal Investigator

Fujisawa Tomotsumi  佐賀大学, 理工学部, 准教授 (60633493)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsラマン光学活性 / 光受容タンパク質 / 低温ラマン分光法 / 低温生物学 / 光受容性タンパク質 / 反応中間体
Outline of Final Research Achievements

This research aimed to utilize Raman optical activity (ROA) spectroscopy for the measurement of the three-dimensional (3D) structure of the reaction intermediates in photoreceptor proteins. We chose the photoreceptors, such as light-driven ion pumps, of micro-organisms and planned to measure ROA of their initial photointermediates trapped at low temperature. As the important step for this ROA measurement, we could measured the low-temperature Raman spectra of the initial intermediates of various photoreceptors and studied their early-time structural changes in the photoreactions. We found a couple of photoreceptors, to which the ROA spectroscopy can be applied, and worked on the development of the low-temperature ROA spectrometer.

Academic Significance and Societal Importance of the Research Achievements

本研究で実施した光受容タンパク質の極低温ラマン測定は、世界的にみても行える研究グループがほとんど無く、独自性の高い構造情報に基づいた光受容タンパク質の研究成果である。また、ラマン光学活性(ROA)分光法は、今日広く利用されるどの分光手法でも測定ができない、タンパク質の反応サイトの詳細な立体構造を捉えられる方法である。本研究はタンパク質研究において"今まで見えなかった構造を観測する"新展開を拓く取り組みである。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (27 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 9 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] オクラハマ州立大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Oklahoma state university(米国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] オクラハマ州立大学(米国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] New Probe for Porcelain Glazes by Luminescence at Near-Infrared Excitation2021

    • Author(s)
      Kamura Shoutaro、Tani Takumi、Matsuo Hideyuki、Onaka Yoshimitsu、Fujisawa Tomotsumi、Unno Masashi
    • Journal Title

      ACS Omega

      Volume: 6 Issue: 11 Pages: 7829-7833

    • DOI

      10.1021/acsomega.1c00322

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport2021

    • Author(s)
      Nakamizo, Y., Fujisawa, T., Kikukawa, T., Okamura, A., Baba, H., Unno, M.
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 23 Issue: 3 Pages: 2072-2079

    • DOI

      10.1039/d0cp05652a

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] “Watching” a Molecular Twist in a Protein by Raman Optical Activity2020

    • Author(s)
      Matsuo, J., Kikukawa, T., Fujisawa, T., Hoff, W. D., Unno, M.
    • Journal Title

      J. Phys. Chem. Lett.

      Volume: 11 Issue: 20 Pages: 8579-8584

    • DOI

      10.1021/acs.jpclett.0c02448

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Low-Temperature Raman Spectroscopy of Halorhodopsin from Natronomonas pharaonis: Structural Discrimination of Blue-Shifted and Red-Shifted Photoproducts2019

    • Author(s)
      Fujisawa Tomotsumi、Kiyota Hayato、Kikukawa Takashi、Unno Masashi
    • Journal Title

      Biochemistry

      Volume: 58 Issue: 40 Pages: 4159-4167

    • DOI

      10.1021/acs.biochem.9b00643

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin2019

    • Author(s)
      Iizuka Azusa、Kajimoto Kousuke、Fujisawa Tomotsumi、Tsukamoto Takashi、Aizawa Tomoyasu、Kamo Naoki、Jung Kwang-Hwan、Unno Masashi、Demura Makoto、Kikukawa Takashi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 10711-10711

    • DOI

      10.1038/s41598-019-47178-5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome2019

    • Author(s)
      Teppei Sato, Takashi Kikukawa, Risako Miyoshi, Kousuke Kajimoto, Chinatsu Yonekawa, Tomotsumi Fujisawa, Masashi Unno, Toshihiko Eki, Yuu Hirose
    • Journal Title

      J. Biol. Chem

      Volume: 294 Issue: 49 Pages: 18909-18922

    • DOI

      10.1074/jbc.ra119.010384

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of the Deprotonated Pyrrole Nitrogen of the Bilin-Based Photoreceptor by Raman Spectroscopy with an Advanced Computational Analysis2019

    • Author(s)
      Osoegawa Shinsuke、Miyoshi Risako、Watanabe Kouhei、Hirose Yuu、Fujisawa Tomotsumi、Ikeuchi Masahiko、Unno Masashi
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 印刷中 Issue: 15 Pages: 3242-3247

    • DOI

      10.1021/acs.jpcb.9b00965

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin2018

    • Author(s)
      Fujisawa Tomotsumi、Abe Masahiro、Tamogami Jun、Kikukawa Takashi、Kamo Naoki、Unno Masashi
    • Journal Title

      FEBS Letters

      Volume: 592 Issue: 18 Pages: 3054-3061

    • DOI

      10.1002/1873-3468.13219

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration2018

    • Author(s)
      Haraguchi Shojiro、Shingae Takahito、Fujisawa Tomotsumi、Kasai Noritaka、Kumauchi Masato、Hanamoto Takeshi、Hoff Wouter D.、Unno Masashi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 115 Issue: 35 Pages: 8671-8675

    • DOI

      10.1073/pnas.1806491115

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Three-dimensional chromophore structures in proton-pumping microbial rhodopsins from Raman optical activity2020

    • Author(s)
      藤澤知績
    • Organizer
      第58回生物物理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 低温ラマン分光法によるナトリウムポンプロドプシンの初期構造変化の研究2019

    • Author(s)
      藤澤知績
    • Organizer
      ISSP ワークショップ「レチナールタンパク質の光機能発現の物理と化学」
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ラマン光学活性分光法の光受容性タンパク質への応用2019

    • Author(s)
      藤澤知績
    • Organizer
      第42回溶液化学シンポジウム・プレシンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Raman optical activity observes a clear structural change of active site caused by trimer formation of halorhodopsin2019

    • Author(s)
      小川将吾、藤澤知績、菊川峰志、海野雅司
    • Organizer
      第42回溶液化学シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] Near-IR Raman optical activity Spectroscopy of Inward proton pump rhodopsin2019

    • Author(s)
      黒岩亮介、藤澤知績、菊川峰志、海野雅司
    • Organizer
      第42回溶液化学シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] ラマン光学活性で観るハロロドプシン多量体形成における活性部位の構造変化2019

    • Author(s)
      小川将吾、藤澤知績、菊川峰志、海野雅司
    • Organizer
      日本生物物理学会第57回年会
    • Related Report
      2019 Research-status Report
  • [Presentation] ラマン光学活性による光駆動内向きプロトンポンプの研究2019

    • Author(s)
      黒岩亮介、藤澤知績、菊川峰志、海野雅司
    • Organizer
      日本生物物理学会第57回年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Raman Optical Activity Spectroscopy of a New Microbial Rhodopsin with Inward Proton Transport Activity2018

    • Author(s)
      藤澤知績
    • Organizer
      ICORS2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of active site in microbial rhodopsins using Raman optical activity2018

    • Author(s)
      藤澤知績
    • Organizer
      SciX2018
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Distinctively small distortion of retinal chromophore in K intermediate of proteorhodopsin observed by low-temperature Raman spectroscopy2018

    • Author(s)
      藤澤知績
    • Organizer
      第56回生物物理学会年会
    • Related Report
      2018 Research-status Report
  • [Book] Vibrational optical activity spectroscopy; In Molecular and Laser Spectroscopy: Advances and Applications: Volume 22020

    • Author(s)
      Tomotsumi Fujisawa, Masashi Unno
    • Total Pages
      42
    • Publisher
      Elsevier
    • ISBN
      9780128188705
    • Related Report
      2020 Annual Research Report
  • [Book] 理工系の大学基礎化学2019

    • Author(s)
      相樂隆正/共編著 海野雅司/共編著
    • Total Pages
      210
    • Publisher
      培風館
    • Related Report
      2019 Research-status Report
  • [Book] Encyclopedia of Analytical Chemistry2019

    • Author(s)
      Tomotsumi Fujisawa
    • Total Pages
      18
    • Publisher
      Wiley
    • Related Report
      2019 Research-status Report
  • [Remarks] 教員(准教授)|藤澤知績

    • URL

      http://biophysics.chem.saga-u.ac.jp/Fujisawa_webpage/Tfujisawa.html

    • Related Report
      2018 Research-status Report
  • [Patent(Industrial Property Rights)] 海苔の品質評価方法、および海苔の品質評価装置2020

    • Inventor(s)
      海野雅司、川村嘉応、藤澤知績、木村 圭
    • Industrial Property Rights Holder
      海野雅司、川村嘉応、藤澤知績、木村 圭
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-097092
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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