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Elucidation of structure and polarity inside lipid bilayer membranes as studied by laser spectroscopy of lipid bilayer membrane nanodiscs

Research Project

Project/Area Number 18K05043
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 32010:Fundamental physical chemistry-related
Research InstitutionToyama Prefectural University (2019-2020)
Gakushuin University (2018)

Principal Investigator

Takaya Tomohisa  富山県立大学, 工学部, 講師 (70466796)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords蛍光分光 / 脂質二重膜 / 蛍光プローブ / 膜物性 / 極性 / 粘度 / ゲルろ過 / 分子分光 / 電荷移動
Outline of Final Research Achievements

This study was aimed at the development of a method for evaluating the polarity distribution inside lipid bilayer biomembranes using laser-induced fluorescence spectroscopy. Lipid bilayer membrane nanodiscs composed of several hundred lipid molecules were chosen as a model of biomembranes. A fluorescence probe sensitive to the polarity of solvents, 9,9'-bianthryl, was encapsulated in lipid membrane nanodiscs. Fluorescence spectra of 9,9'-bianthryl in lipid membrane nanodiscs indicate that their interior has polarity similar to that of alcohols. Technical improvements in preparing lipid membrane nanodiscs, such as fine control of the disc diameter and a significant increase of the disc yield are necessary for obtaining detailed information on inhomogeneity of the polarity.

Academic Significance and Societal Importance of the Research Achievements

細胞膜の中でさまざまな生化学反応が効率よく結びつきながら進む理由として,細胞膜を形づくる分子が膜内で何らかの特殊な集合体を構成しているという仮説が提起されている.「細胞膜中で分子が集合することで,膜の物理的・化学的性質がどのように分布し,反応に影響を与えるか」を調べるための新しい基礎分析方法を開発し,提案したことが本研究の学術的意義である.細胞膜の性質が正しく分かれば,正確かつ効率的にはたらく薬の開発などに役立つと期待される.

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (3 results)

All 2020 2019

All Presentation (3 results)

  • [Presentation] 時間分解けい光分光法による脂質二重膜ナノディスクの粘度評価2020

    • Author(s)
      塩野晴菜,高門輝,高屋智久,中村浩之,岩田耕一
    • Organizer
      分子科学会オンライン討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脂質二重膜ナノディスクの粘度評価2020

    • Author(s)
      塩野晴菜,林春菜,高門輝,高屋智久,中村浩之,岩田耕一
    • Organizer
      2020年度日本分光学会年次講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脂質二重膜ナノディスクの粘度評価の試み2019

    • Author(s)
      塩野晴菜,林春菜,高門輝,高屋智久,中村浩之,岩田耕一
    • Organizer
      分子科学討論会2019名古屋
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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