Study on the key reaction mechanisms and chemical factors for high-performance luminescence in bioluminescence systems
Project/Area Number |
18K05075
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 33010:Structural organic chemistry and physical organic chemistry-related
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Research Institution | The University of Electro-Communications |
Principal Investigator |
Hirano Takashi 電気通信大学, 大学院情報理工学研究科, 教授 (20238380)
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Project Period (FY) |
2018-04-01 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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Keywords | 生物発光 / ホタル / ウミホタル / ルシフェリン / ルシフェラーゼ / 反応機構 / 酸素化 / 化学励起 / オキシルシフェリン / 酵素環境場 |
Outline of Final Research Achievements |
Luciferin-luciferase (L-L) reactions of firefly and ostracod bioluminescence are widely applied for biological analysis and imaging. To support the development of their applications, we need to understand the mechanisms of the L-L reactions made up by several elemental processes. For the purpose, we carried out mechanistic studies on the three important processes, oxygenation, chemiexcitation, and emission from the excited oxyluciferins in the L-L reactions. The results provide information on the molecular mechanisms to design luciferin derivatives with high-performance luminescence property.
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Academic Significance and Societal Importance of the Research Achievements |
生物発光は化学反応により光子を生み出す特徴を持ち、生命科学分野の発光分析やイメージング技術を支える。生物発光の分析利用を発展させるには、基盤となる反応機構の理解が不可欠であり、本研究で得られた素反応過程の分子機構に関する知見は、高性能な生物発光を模倣した発光基質の分子設計指針を提供するため学術的意義が大きい。さらに、生物発光の分析利用法の高性能化に寄与することで、医療を含めた生命科学分野の発展にも貢献する。
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Spectroscopic properties of push-pull 2-(4-carboxyphenyl-6-dimethylaminobenzothiazole derivatives in solution and the solid state2019
Author(s)
Takahashi, Yusuke; Uehara, Takuya; Matsuhashi, Chihiro; Yamaji, Minoru; Mutai, Toshiki; Yoshikawa, Isao; Houjou, Hirohiko; Kitagawa, Kota; Suenobu, Tomoyoshi; Maki, Shojiro; Hirano, Takashi
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Journal Title
Journal of Photochemistry and Photobiology, A: Chemistry
Volume: 376
Pages: 324-332
DOI
Related Report
Peer Reviewed
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