| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we constructed artificial enzymes that catalyze cascade reactions by immobilizing multiple metal complexes and enzymes in the internal space of protein crystals formed in living cells. We have prepared the polyhedra crystals immobilizing Pd and Ir complexes. The ICP-MS result indicated that Pd and Ir complexes were immobilized in the crystals. Molecular design for catalytic reaction activity will be carried out in the future.
We have constructed the 38-amino acid deletion mutants, which forms interlinked hollow nanocages with a diameter of 5 nm in the crystals. The mutant crystal can encapsulate lipase and alcohol dehydrogenase. The composite crystal enhances the reactivity of the cascade reaction. The higher reactivity is because of the substrate and intermediate efficiently diffusing through the extended channels designed within the nanoporous crystal.
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