Stem cell culture and differentiation on polymeric surface immobilized with bioactive nanosegments
| Project/Area Number |
18K05251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 35020:Polymer materials-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Higuchi Akon 国立研究開発法人理化学研究所, 開拓研究本部, 客員研究員 (30189766)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 幹細胞培養 / 生体適合性材料 / オリゴペプチド / 分化 / 細胞培養基板 / 細胞接着因子ペプチド / 弾性 / バイオマテリアル / 幹細胞 / 培養 / 細胞接着因子 |
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| Outline of Final Research Achievements |
The objectives of this study were (1) to design stem cell culture substrates that take into account both physical and biochemical factors, and to investigate the possibility of mass culturing ES and iPS cells while maintaining their pluripotency, (2) to determine the optimal substrate stiffness/elasticity and the optimal extracellular matrix (ECM) and oligopeptide for efficient differentiation into appropriate tissue cells (osteoblasts, cardiomyocytes and mesenchymal stem cells). Polyvinyl alcohol hydrogels with an elastic modulus of around 25 kPa were found to be the most suitable substrates for ES and iPS cell culture. The cell adhesion peptides (peptides derived from laminin β4 with double-stranded structure, joint groups and basic lysine) on the nanosegment-immobilized cell culture substrates were found to be the optimal for peptide-grafted hydrogels for ES and iPS cell culture and differentiation.
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| Academic Significance and Societal Importance of the Research Achievements |
再生医療の発展のためには、特定の組織細胞に幹細胞を高効率で分化させること、さらにヒトiPS細胞並びにES細胞を多分化能状態で、細胞増殖を行なうことが必須である。本研究成果は、幹細胞培養並びに分化に最適な細胞培養基板の剛性/弾性の同定を行なった。さらに、最適な剛性/弾性を有する細胞培養基板上に固定化された最適なナノセグメント（ECM並びに細胞接着因子ペプチド）を同定したことが、学術的意義であり独創的な点である。本研究成果の概念が普及することにより、幹細胞並びにＥＳ（胚性幹）細胞の培養並びに分化が容易になり、再生医療に貢献することが期待される（社会的意義）。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Effect of cell culture biomaterials for completely xeno-free generation of human induced pluripotent stem cells2020
Author(s)
Tzu-Cheng Sung, Hsing-Fen Li, Akon Higuchi,* S. Suresh Kumar, Qing-Dong Ling, Yu-Wen Wu, Thierry Burnouf, Michiyo Nasu, Akihiro Umezawa, Kuei-Fang Lee, Han-Chow Wang, Yung Chang, Shih-Tien Hsu
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Journal Title
Biomaterials
Volume: 230
Pages: 119638-119638
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The design of a thermoresponsive surface for the continuous culture of human pluripotent stem cells2019
Author(s)
Tzu-Cheng Sung, Jia-Sin Yang, Chih-Chen Yeh, Ya-Chu Liu, Yi-Peng Jiang, Ming-Wei Lu, Qing-Dong Ling, S. Suresh Kumar, Yung Chang, Akihiro Umezawa, Hao Chen, Akon Higuchi,*
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Journal Title
Biomaterials
Volume: 221
Pages: 119411-119411
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] The effect of human platelet lysate on the differentiation ability of human adipose-derived stem cells cultured on ECM-coated surfaces2019
Author(s)
Yan Gao, Nien-Ju Ku, Tzu-Cheng Sung, Akon Higuchi,* Chi-Sheng Hung, Henry Hsin-chung Lee, Qing-Dong Ling, Nai-Chen Cheng, Akihiro Umezawae, Lassina Barro, Thierry Burnouf, Qingsong Ye and Hao Chen
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Journal Title
Journal of Materials Chemistry B
Volume: 7
Issue: 45
Pages: 7110-7119
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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