Development of molecules for non-fluorescent imaging of cell organelles and membrane domains

• Project/Area Number: 18K05312
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: Momotake Atsuya 筑波大学, 数理物質系, 准教授 (70375369)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 無蛍光イメージング分子 / 非線形光学顕微鏡 / 細胞膜 / ジャイアントベシクル / アゾベンゼン / 両親媒性分子 / 光和周波・第二高調波 / 無蛍光イメージング / マルチモーダルイメージング / 細胞膜イメージング / Pheophorbide a / SFG顕微鏡 / アゾ色素 / 無発光イメージング / 第二高調波発生

Outline of Final Research Achievements

Dye-based Sum Frequency Generation (SFG) imaging using non-fluorescent molecules dedicated to nonlinear optical microscopy has yielded the following results:1) Morphological changes in giant vesicles (GVs), a cellular model, were monitored and SFG-active vesicles that arise inside GVs were detected for the first time. 2) A detailed comparison of the properties of SFG imaging and coherent anti-Stokes Raman scattering (CARS) imaging of lipid bilayers in dopaminergic neurons shows their applicability to live cell tracking of intracellular lipid structures such as lipid droplets under the cell membrane.3) The first detection of SFG inside hippocampal cells suggests that the molecule in this study localizes to the inner membrane of the lipid bilayer that arises inside the cell.

Academic Significance and Societal Importance of the Research Achievements

以前の研究で開発した無蛍光性非線形光学専用分子Ap3により、初めて2光子蛍光とSHGの同時イメージングが成功したが、Ap3がイメージングできるのは細胞膜の脂質二重膜の外膜部分だけであった。本研究で開発された分子BOをまず人工細胞であるジャイアントベシクルに取り込ませると、膜が変形し、ジャイアントベシクル内部からSFG信号が検出される過程を捉えることに成功した。また細胞内部からもSFG信号が検出されたことから、BOは細胞内部に生じた脂質二重膜の内膜に局在することが示唆された。本研究により脂質二重層の外膜と内膜の違いを認識しながら膜全体のダイナミクスをモニターすることが可能となった。

Research Products

• [Journal Article] Nature of a H2O Molecule Confined in the Hydrophobic Interface between the Heme and G-Quartet Planes in a Heme-DNA Complex (2022)
  • Author(s): Nakajima Yusuke、Momotake Atsuya、Suzuki Akihiro、Neya Saburo、Yamamoto Yasuhiko
  • Journal Title: Biochemistry Volume: － Issue: 7 Pages: 523-534
  • DOI: 10.1021/acs.biochem.1c00751
  • Peer Reviewed
• [Journal Article] Molecular recognition of G-quadruplex DNA by Pheophorbide a (2021)
  • Author(s): Okamoto China、Momotake Atsuya、Kobayashi Masami、Yamamoto Yasuhiko
  • Journal Title: Chemistry Letters Volume: - Issue: 6 Pages: 1278-1281
  • DOI: 10.1246/cl.210130
  • NAID: 130008053396
  • Peer Reviewed
• [Journal Article] Effects of Heme Electronic Structure and Local Heme Environment on Catalytic Activity of a Peroxidase-Mimicking Heme-DNAzyme (2021)
  • Author(s): Hagiwara Shota、Momotake Atsuya、Ogura Takashi、Yanagisawa Sachiko、Suzuki Akihiro、Neya Saburo、Yamamoto Yasuhiko
  • Journal Title: Inorganic Chemistry Volume: 60 Issue: 15 Pages: 11206-11213
  • DOI: 10.1021/acs.inorgchem.1c01179
  • Peer Reviewed
• [Journal Article] A cationic copolymer as a cocatalyst for a peroxidase-mimicking heme-DNAzyme (2021)
  • Author(s): Araki Haruka、Hagiwara Shota、Shinomiya Ryosuke、Momotake Atsuya、Kotani Hiroaki、Kojima Takahiko、Ochiai Takuro、Shimada Naohiko、Maruyama Atsushi、Yamamoto Yasuhiko
  • Journal Title: Biomaterials Science Volume: 9 Issue: 18 Pages: 6142-6152
  • DOI: 10.1039/d1bm00949d
  • Peer Reviewed
• [Journal Article] Structural and functional characterization of complexes between heme and dimeric parallel G-quadruplex DNAs (2021)
  • Author(s): Okamoto China、Momotake Atsuya、Yamamoto Yasuhiko
  • Journal Title: Journal of Inorganic Biochemistry Volume: 216 Pages: 111336-111336
  • DOI: 10.1016/j.jinorgbio.2020.111336
  • Peer Reviewed
• [Journal Article] Effect of the Electron Density of the Heme Fe Atom on the Nature of Fe-O2 Bonding in Oxy Myoglobin (2020)
  • Author(s): Yamamoto Yasuhiko、Hasegawa Kazuyasu、Shibata Tomokazu、Momotake Atsuya、Ogura Takashi、Yanagisawa Sachiko、Neya Saburo、Suzuki Akihiro、Kobayashi Yasuhiro、Saito Makina、Seto Makoto、Ohta Takehiro
  • Journal Title: Inorganic Chemistry Volume: 60 Issue: 2 Pages: 1021-1027
  • DOI: 10.1021/acs.inorgchem.0c03123
  • Peer Reviewed
• [Journal Article] Photogeneration of Reactive Oxygen Species from Water-Soluble Phthalocyanine Derivatives Bound to a G-Quadruplex DNA (2020)
  • Author(s): Uchiyama Mami、Momotake Atsuya、Ikeue Takahisa、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 93 Issue: 12 Pages: 1504-1508
  • DOI: 10.1246/bcsj.20200178
  • NAID: 130007955892
  • Peer Reviewed
• [Journal Article] Stepwise binding of a cationic phthalocyanine derivative to an all parallel-stranded tetrameric G-quadruplex DNA (2020)
  • Author(s): Uchiyama Mami、Okamoto China、Momotake Atsuya、Ikeue Takahisa、Yamamoto Yasuhiko
  • Journal Title: Journal of Inorganic Biochemistry Volume: 213 Pages: 111270-111270
  • DOI: 10.1016/j.jinorgbio.2020.111270
  • Peer Reviewed
• [Journal Article] Monitoring the morphological evolution of giant vesicles by azo dye-based sum-frequency generation (SFG) microscopy (2020)
  • Author(s): Momotake Atsuya、Mizuguchi Takaha、Hishida Mafumi、Yamamoto Yasuhiko、Yasui Masato、Nuriya Mutsuo
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 186 Pages: 110716-110716
  • DOI: 10.1016/j.colsurfb.2019.110716
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Intermediates in Peroxidase Catalytic Cycle of a DNAzyme Possessing Heme (2019)
  • Author(s): Shinomiya Ryosuke、Araki Haruka、Momotake Atsuya、Kotani Hiroaki、Kojima Takahiko、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 92 Issue: 10 Pages: 1729-1736
  • DOI: 10.1246/bcsj.20190157
  • NAID: 130007728639
  • Peer Reviewed
• [Journal Article] Photochemical properties of enediyne-cored dendrimers bearing naphthalenes at the periphery (2019)
  • Author(s): Ichino Rina、Momotake Atsuya、Arai Tatsuo
  • Journal Title: Canadian Journal of Chemistry Volume: 97 Issue: 2 Pages: 112-119
  • DOI: 10.1139/cjc-2018-0128
  • Peer Reviewed
• [Journal Article] Structures and Catalytic Activities of Complexes between Heme and All Parallel-Stranded Monomeric G-Quadruplex DNAs (2018)
  • Author(s): Yamamoto Yasuhiko、Araki Haruka、Shinomiya Ryosuke、Hayasaka Kosuke、Nakayama Yusaku、Ochi Kentaro、Shibata Tomokazu、Momotake Atsuya、Ohyama Takako、Hagihara Masaki、Hemmi Hikaru
  • Journal Title: Biochemistry Volume: 57 Issue: 41 Pages: 5938-5948
  • DOI: 10.1021/acs.biochem.8b00792
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Distal Polar Interactions in Myoglobin and Their Structural Consequences (2018)
  • Author(s): Watanabe Miho、Kanai Yuki、Nakamura Shunpei、Nishimura Ryu、Shibata Tomokazu、Momotake Atsuya、Yanagisawa Sachiko、Ogura Takashi、Matsuo Takashi、Hirota Shun、Neya Saburo、Suzuki Akihiro、Yamamoto Yasuhiko
  • Journal Title: Inorganic Chemistry Volume: 57 Issue: 22 Pages: 14269-14279
  • DOI: 10.1021/acs.inorgchem.8b02302
  • Peer Reviewed
• [Journal Article] Characterization of Catalytic Activities and Heme Coordination Structures of Heme?DNA Complexes Composed of Some Chemically Modified Hemes and an All Parallel-Stranded Tetrameric G-Quadruplex DNA Formed from d(TTAGGG) (2018)
  • Author(s): Shinomiya Ryosuke、Katahira Yuya、Araki Haruka、Shibata Tomokazu、Momotake Atsuya、Yanagisawa Sachiko、Ogura Takashi、Suzuki Akihiro、Neya Saburo、Yamamoto Yasuhiko
  • Journal Title: Biochemistry Volume: 57 Issue: 41 Pages: 5930-5937
  • DOI: 10.1021/acs.biochem.8b00793
  • Peer Reviewed
• [Presentation] 四重鎖DNAのキラターゼ活性 (2022)
  • Author(s): 鳥越果林，岡本千奈，百武篤也，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] フェオホルバイドaとグアニン四重鎖DNAの相互作用 (2022)
  • Author(s): 岡本千奈，百武篤也，小林正美，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] ヘム-DNA酵素におけるヘムとG-カルテットの接触界面に存在する水分子の解析 (2022)
  • Author(s): 中島優輔，百武篤也，鈴木秋弘，根矢三郎，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] アニオン性フタロシアニン-四重鎖DNA複合体の解析 (2022)
  • Author(s): 小田原佑典，百武篤也，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] 熱安定性が高いヘム-DNA酵素のペルオキシダーゼ活性の解析 (2022)
  • Author(s): 深澤大輝，百武篤也，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] Characterization of a Complex Between Heme(Fe3+) and a G-quadruplex DNA (2021)
  • Author(s): Y. Nakajima, S. Neya, A. Suzuki, A. Momotake, and Y. Yamamoto
  • Organizer: IEEE International Symposium on Mixed and Augmented Reality (ISMAR) 2021
  • Int'l Joint Research
• [Presentation] Molecular Recognition of G-Quadruplex DNAs by Tetrapyrrole Macrocyclic Ligands (2021)
  • Author(s): C. Okamoto, A. Momotake, and Y. Yamamoto
  • Organizer: IEEE International Symposium on Mixed and Augmented Reality (ISMAR) 2021
  • Int'l Joint Research
• [Presentation] Characterization of Myoglobins Where Exogenous Ligands Replace the Proximal Histidine (2021)
  • Author(s): R. Murata, T. Sugita, T. Matsuo, S. Hirota, S. Yanagisawa, S. Neya, A. Suzuki, O. Shoji, Y. Watanabe, A. Momotake, and Y. Yamamoto
  • Organizer: IEEE International Symposium on Mixed and Augmented Reality (ISMAR) 2021
  • Int'l Joint Research
• [Presentation] Heme Electronic Structure and Local Heme Environment of a Peroxidase-Mimicking Heme-DNAzyme (2021)
  • Author(s): S. Hagiwara, A. Sugahara, R. Shinomiya, S. Neya, A. Suzuki, A. Momotake, and Y. Yamamoto
  • Organizer: IEEE International Symposium on Mixed and Augmented Reality (ISMAR) 2021
  • Int'l Joint Research
• [Presentation] Interaction between Heme and Non-Standard Base Inosine(I)-Quartet (2021)
  • Author(s): A. Momotake, A. Sugahara, K. Torigoe, Y. Nakajima, S. Neya, A. Suzuki, and Y. Yamamoto
  • Organizer: IEEE International Symposium on Mixed and Augmented Reality (ISMAR) 2021
  • Int'l Joint Research
• [Presentation] Interaction between Zn(II) Phthalocyanine Derivatives and a G-Quadruplex DNA (2021)
  • Author(s): S. Homma, M. Uchiyama, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: IEEE International Symposium on Mixed and Augmented Reality (ISMAR) 2021
  • Int'l Joint Research
• [Presentation] Elucidation of the Regulatory Mechanism for Peroxidase Activity of DNAzyme Possessing Heme (2021)
  • Author(s): S. Hagiwara, R. Shinomiya, A. Momotake, S. Neya, A. Suzuki, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] Characterization of a Complex Consisting of Paramagnetic Heme(Fe3+) and G-Guadruplex DNA Formed from d(TAGGGTGGGTTGGGTGIG) (2021)
  • Author(s): Y. Nakajima, S. Neya, A. Suzuki, A. Momotake, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] Study of Myoglobin Reconstituted with a Chemically-Modified Heme Cofactor Possessing a Trifluoro Methyl Group at 5-Meso Position (2021)
  • Author(s): R. Murata, A. Momotake, A. Suzuki, T. Matsuo, S. Hirota, S. Neya, M. Senda, T. Senda, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] Interaction between Heme and I-quartet in a Heme-DNAzyme (2021)
  • Author(s): K. Torigoe, A. Momotake, S. Neya, S. Yanagisawa, T. Ogura, A. Suzuki, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] Study on the Effect of G-Quadruplex DNA on Photochemical Behaviors of Cationic Phthalocyanine Derivatives (2021)
  • Author(s): M. Uchiyama, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] Molecular Recognition of G-Quadruplex DNA by Pheophorbide a (2021)
  • Author(s): C. Okamoto, A. Momotake, M. Kobayashi, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] Interaction of Cationic Phthalocyanine Derivatives with Guanine Nucleobases (2021)
  • Author(s): S. Homma, M. Uchiyama, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021 (Pacifichem 2021)
  • Int'l Joint Research
• [Presentation] 四重鎖 DNA とフタロシアニン誘導体の複合体の解析 (2020)
  • Author(s): 内山真見、岡本千奈、百武篤也、山本泰彦、池上崇久
  • Organizer: 第59回NMR討論会(2020)
• [Presentation] ヘム-DNA 複合体のペルオキシダーゼ活性におけるPush 効果とPull 効果の相関関係の解析 (2020)
  • Author(s): ○萩原 翔太・篠宮 僚介・百武 篤也・鈴木 秋弘・根矢 三郎・山本 泰彦
  • Organizer: 日本化学会 第100春季年会 (2020)
• [Presentation] ヒトテロメア関連塩基配列のDNA が2 分子で形成する平行型四重鎖DNA およびそのヘムとの複合体の研究 (2020)
  • Author(s): ○岡本 千奈・篠宮 僚介・山田 果央・百武 篤也, 山本 泰彦
  • Organizer: 日本化学会 第100春季年会 (2020)
• [Presentation] カチオン性共重合体によるヘム-DNA 複合体のペルオキシダーゼ活性の増大 (2020)
  • Author(s): 荒木 はるか・百武 篤也・〇山本 泰彦・落合 拓郎・嶋田 直彦・丸山 厚
  • Organizer: 日本化学会 第100春季年会 (2020)
• [Presentation] ヘム－DNA 複合体のイミダゾール付加物の解析 (2020)
  • Author(s): 〇中島 優輔・百武 篤也・山本 泰彦
  • Organizer: 日本化学会 第100春季年会 (2020)
• [Presentation] 四重鎖DNAとフタロシアニン誘導体の複合体 (2020)
  • Author(s): 内山 真見、柴田 友和、○百武 篤也、池上 崇久、松井 亨、山本 泰彦
  • Organizer: 日本化学会 第100春季年会 (2020)
• [Presentation] メソ位にトリフルオロメチル基を導入したヘムをもつミオグロビンの研究 (2020)
  • Author(s): 〇村田 莉恵・百武 篤也・山本 泰彦・鈴木 秋弘・松尾 貴史・廣田 俊・根矢 三郎
  • Organizer: 日本化学会 第100春季年会 (2020)
• [Presentation] Deoxyribozyme possessing Heme (2019)
  • Author(s): ○Yasuhiko Yamamoto, Haruka Araki, Kosuke Hayasaka, Shunpei Nakamura,Tomokazu Shibata, Atsuya Momotake, and Hikaru Hemmi
  • Organizer: Experimental Nuclear Magnetic Resonance Conference
  • Int'l Joint Research
• [Presentation] Structures and Catalytic Activities of DNA Complexes Consisting of Chemically Modified Heme and Tetrameric G-Quadruplex DNA (2019)
  • Author(s): Ryosuke Shinomiya, ○Atsuya Momotake, Yuya Katahira, Haruka Araki, Tomokazu Shibata, Sachiko Yanagisawa, Takashi Ogura, Akihiro Suzuki, Saburo Neya, and Yasuhiko Yamamoto
  • Organizer: 15th International Symposium on Applied Bioinorganic Chemistry
  • Int'l Joint Research
• [Presentation] ヘムをもつ DNA 酵素 (2019)
  • Author(s): ◯山本 泰彦, 篠宮 僚介, 小谷 弘明, 荒木 はるか,柴田 友和, 百武 篤也, 柳澤 幸子, 小倉 尚志, 逸見 光, 鈴木 秋弘, 根矢 三郎 , 小島 隆彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 非線形光学イメージングに向けた無蛍光性分子プローブの開発と応用 (2019)
  • Author(s): ○百武篤也, 水口高翔, 塗谷睦生, 安井正人, 山本泰彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 四重鎖 DNA の形成様式がヘムをもつ DNA 酵素の性質に与える影響 (2019)
  • Author(s): ◯荒川和基, 山田果央, 片平裕弥, 越智健太郎, 中山優作, 荒木はるか, 篠宮僚介, 百武篤也, 山本泰彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 3'末端に I-カルテットをもつ四重鎖 DNA とヘムの複合体の研究 (2019)
  • Author(s): ◯菅原綾, 篠宮僚介, 木下真志, 柴田友和, 百武篤也, 山本泰彦, 柳澤幸子, 小倉尚志, 鈴木秋弘, 根矢三郎
  • Organizer: 第46回生体分子科学討論会
• [Presentation] .ヘム鉄の軸配位子としてイミダゾールをもつミオグロビンの研究 (2019)
  • Author(s): ◯杉田達郎, 百武篤也, 山本泰彦, 松尾貴史, 廣田俊, 鈴木秋弘, 根矢三郎, 荘司長三, 渡辺芳人
  • Organizer: 第46回生体分子科学討論会
• [Presentation] Heme-DNAzyme (2019)
  • Author(s): ○Y. Yamamoto, R. Shinomiya, H. Araki, T. Shibata, A. Momotake, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya and H. Hemmi
  • Organizer: Commemorative International Symposium of the Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Heme Meets DNA. (2019)
  • Author(s): ○Yasuhiko Yamamoto, Ryosuke Shinomiya, Hiroaki Kotani, Haruka Araki, Atsuya Momotake,Tomokazu Shibata, Sachiko Yanagisawa, Takashi Ogura, Hikaru Hemmi, Akihiro Suzuki,Saburo Neya, Takahiko Kojima
  • Organizer: ICBIC-19
  • Int'l Joint Research
• [Presentation] ヘムをもつDNA 酵素の酸化触媒機構 (2019)
  • Author(s): ○山本 泰彦・篠宮 僚介・小谷 弘明・荒木 はるか・柴田 友和・百武 篤也・柳澤 幸子・小倉 尚志・鈴木 秋弘・根矢 三郎・小島 隆彦
  • Organizer: 題１３回バイオ関連化学シンポジウム
• [Presentation] Enhancement of peroxidase activity of heme-DNAzyme through interaction with cationic copolymers (2019)
  • Author(s): ○Haruka Araki, Atsuya Momotake, Yasuhiko Yamamoto, Takuro Ochiai, Naohiko Shimada, and Atsushi Maruyama
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] DNAzyme Possessing Heme (2019)
  • Author(s): ○Yasuhiko Yamamoto, Ryosuke Shinomiya, Haruka Araki, Tomokazu Shibata, Atsuya Momotake, Sachiko Yanagisawa, Takashi Ogura, Akihiro Suzuki, and Saburo Neya
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Complexes between G-quadruplex DNA and Phthalocyanines (2019)
  • Author(s): ○Mami Uchiyama, Tomokazu Shibata, Atsuya Momotake, Takahisa Ikeue, and Yasuhiko Yamamoto
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Mechanism of Peroxidase Activity of a Complex between G quadruplex DNA and Heme (2019)
  • Author(s): ○Aya Sugahara, Ryosuke Shinomiya, Hiroaki Kotani, Haruka Araki, Tomokazu Shibata, Atsuya Momotake, Sachiko Yanagisawa, Takashi Ogura, Akihiro Suzuki, Saburo Neya, Takahiko Kojima, and Yasuhiko Yamamoto
  • Organizer: 第52回酸化反応討論会
• [Presentation] Electronic Control of Myoglobin Function (2019)
  • Author(s): ○Tatsuro Sugita, Takashi Matsuo, Shun Hirota, Sachiko Yanagisawa, Takashi Ogura, Osami Shoji, Yoshihito Watanabe, Akihiro Suzuki, Saburo Neya, Atsuya Momotake, and Yasuhiko Yamamoto
  • Organizer: XVII-ISBS-2019
  • Int'l Joint Research
• [Presentation] 非線形顕微鏡に用いる無発光性分子プローブの開発 (2019)
  • Author(s): 百武篤也
  • Organizer: 日本化学会中国四国支部 島根地区化学講演会―機器分析 のすゝめ ―
  • Invited
• [Presentation] 四重鎖DNAの形成様式がヘムをもつDNA酵素の性質に与える影響 (2019)
  • Author(s): 山田 果央・荒川 和基・篠宮 僚介・荒木 はるか・柴田 友和・百武 篤也・山本 泰彦
  • Organizer: 日本化学会第99春季年会(2019)
• [Presentation] ヘムをもつDNA酵素のヘム鉄に軸配位子として結合する水分子の性質 (2019)
  • Author(s): 山本 泰彦・篠宮 僚介・荒木 はるか・柴田 友和・百武 篤也・柳澤 幸子・小倉 尚志・鈴木 秋弘・根矢 三郎・逸見 光
  • Organizer: 日本化学会第99春季年会(2019)
• [Presentation] ヘムをもつDNA酵素におけるペルオキシダーゼ活性発現機構の解明 (2019)
  • Author(s): 篠宮 僚介・百武 篤也・柳澤 幸子・鈴木 秋弘・根矢 三郎・小谷 弘明・小島 隆彦・山本 泰彦
  • Organizer: 日本化学会第99春季年会(2019)
• [Presentation] Deoxyribozymes Composed of Heme and G-quadruplex DNAs (2018)
  • Author(s): Yasuhiko Yamamoto, Haruka Araki, Mami Uchiyama, Kosuke Hayasaka, Ryosuke Shinomiya, Tomokazu Shibata, Atsuya Momotake, Masaki Hagihara, and Hikaru Hemmi
  • Organizer: Advances in Noncanonical Nucleic Acids2018
  • Int'l Joint Research
• [Presentation] REDOX-CATALYZING DeoxyribozymeS Composed of Heme and DNAS (2018)
  • Author(s): Yasuhiko Yamamoto, Haruka Araki, Ryosuke Shinomiya, Kosuke Hayasaka, Tomokazu Shibata, Atsuya Momotake, SACHIKO YANAGISAWA, TAKASHI OGURA, Hikaru Hemmi, AKIHIRO SUZUKI, SABURO NEYA
  • Organizer: AsBIC9 9th Asian Biological Inorganic Chemistry Conference
  • Int'l Joint Research
• [Presentation] Structure-function investigation of deoxyribozymes composed of heme and G-quadruplex DNAs (2018)
  • Author(s): Yasuhiko Yamamoto, Haruka Araki, Kosuke Hayasaka, Ryosuke Shinomiya, Yusaku Nakayama, Kentaro Ochi, Tomokazu Shibata, Atsuya Momotake, Masaki Hagihara, Dipankar Sen, Hikaru Hemmi
  • Organizer: 第57回NMR討論会
  • Int'l Joint Research
• [Book] “Structures and Catalytic Activities of Complexes between Heme and DNA”, Handbook of Chemical Biology of Nucleic Acids, Ed. N. Sugimoto (2022)
  • Author(s): Y. Yamamoto and A. Momotake
  • Publisher: Springer
• [Book] 核酸科学ハンドブック「核酸二重鎖結合分子」 (2020)
  • Author(s): 山本泰彦、百武篤也、日本核酸化学会、杉本 直己編
  • Publisher: 講談社
  • ISBN: 9784065207864