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Turn-on fluorescent labeling of target protein at a specific lysine residue and formation of protein cross-link with fluorescence for therir application to protein studies

Research Project

Project/Area Number 18K05319
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37010:Bio-related chemistry
Research InstitutionKyushu University

Principal Investigator

Aso Mariko  九州大学, 薬学研究院, 准教授 (30201891)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsリジン修飾 / 環境応答型蛍光 / 反応性核酸 / ソルバトクロミック蛍光 / 蛋白質修飾 / 蛍光 / リジン残基 / 蛋白質 / リジン / 蛍光修飾 / DNA
Outline of Final Research Achievements

We investigated new molecules which convert lysine residue in target protein into environment sensitive fluorophore and provide fluorescent-labeled proteins to monitor protein interaction. Fluorescent labeling of nucleic acid binding protein proceeded with the molecule carrying nucleic acid. Difference in fluorescence intensities was observed between modified proteins in the free and DNA bound forms. Introduction of substituents into fluorophore provided new molecules and fluorophore more stable than the original compound was obtained. We also studied molecules which convert lysine into fluorophore with other functions.

Academic Significance and Societal Importance of the Research Achievements

今回開発したリジン修飾分子は蛋白質親和性の核酸部をもち、標的蛋白質に結合し、緩和な条件でリジン残基を環境応答型蛍光基に変換することができた。修飾蛋白質は核酸の結合の有無により、蛍光強度の変化がみられることが分かり、蛋白質相互作用の検出する分子設計の妥当性を示した。また安定性が向上した蛍光分子や異なる物性を持つ蛍光分子を見出し、これらを生成する修飾分子の合成法を確立した。これらの成果により本手法を用いた蛋白質修飾の更なる展開が可能となった。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (13 results)

All 2023 2022 2021 2020 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (11 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Synthesis of dendritic bisphosphonates as bone targeting ligands2018

    • Author(s)
      Shimoda Kazuma、Mitsuoka Takahiro、Ueda Kenta、Suemune Hiroshi、Hirai Go、Aso Mariko
    • Journal Title

      Tetrahedron Letters

      Volume: 59 Issue: 51 Pages: 4528-4531

    • DOI

      10.1016/j.tetlet.2018.11.028

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Tumor-Imaging Using a Thermo-Responsive Molecule with an Emissive Aminoquinoline Derivative2018

    • Author(s)
      Araki Takeru、Fuchi Yasufumi、Murayama Shuhei、Shiraishi Ryoma、Oyama Tokimi、Aso Mariko、Aoki Ichio、Kobayashi Shigeki、Yamada Ken-ichi、Karasawa Satoru
    • Journal Title

      Nanomaterials

      Volume: 8 Issue: 10 Pages: 782-782

    • DOI

      10.3390/nano8100782

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] リジン残基をソルバトクロミック蛍光基へ変換する標的親和性ベンズアルデヒド誘導体の合成と評価2023

    • Author(s)
      麻生 真理子, 劉 怡萱, 阿部 由紀子, 田畑 香織
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 反応性核酸を用いた蛋白質ソルバトクロミック蛍光修飾-蛍光基の物性、修飾反応に及ぼす置換基の影響2022

    • Author(s)
      麻生真理子, 劉 怡萱, 堂薗拓真, 田畑香織, 阿部由紀子
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 環境応答型solvatochromic蛍光分子で修飾したDNA結合性蛋白質の評価2021

    • Author(s)
      麻生 真理子, 田畑 香織, 阿部 由紀子, 谷口 陽祐, 佐々木 茂貴
    • Organizer
      第15回バイオ関連化学シンポジウム
    • Related Report
      2021 Research-status Report
  • [Presentation] 反応性核酸を用いた蛋白質蛍光修飾法の開発-構造、物性、反応性相関の検討-2021

    • Author(s)
      劉怡萱, 阿部由紀子, 麻生真理子
    • Organizer
      第38回日本薬学会九州山口支部大会
    • Related Report
      2021 Research-status Report
  • [Presentation] DNA-蛋白質クロスリンク形成に応用可能な反応性核酸の開発2021

    • Author(s)
      堂薗拓真, 金城綾香, 阿部由紀子, 麻生真理子
    • Organizer
      第38回日本薬学会九州山口支部大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 反応性核酸を用いた蛋白質のTurn-on型蛍光修飾法の開発2020

    • Author(s)
      阿部 由紀子、劉 怡萱、麻生 真理子
    • Organizer
      第14回バイオ関連化学シンポジウム
    • Related Report
      2020 Research-status Report
  • [Presentation] 反応性核酸を用いた環境応答型solvatochromic蛍光分子の蛋白質への導入と修飾蛋白質の評価2020

    • Author(s)
      麻生 真理子、金城 綾香、田畑 香織、阿部 由紀子、谷口 陽祐、佐々木 茂貴
    • Organizer
      第14回バイオ関連化学シンポジウム
    • Related Report
      2020 Research-status Report
  • [Presentation] 反応性核酸を用いた、ターンオン蛍光を伴う部位特異的リジン修飾2019

    • Author(s)
      麻生真理子、金城 綾香、阿部 由紀子、谷口 陽祐、佐々木 茂貴
    • Organizer
      第13回バイオ関連化学シンポジウム
    • Related Report
      2019 Research-status Report
  • [Presentation] Labeling of DNA-interacting protein using oligodeoxynucleotides that induce solvatochromic fluorophore2019

    • Author(s)
      Mariko Aso, Ayaka Kinjo, Yukiko Abe, Yosuke Taniguchi, Shigeki Sasaki
    • Organizer
      The 46 th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Synthesis and Reaction of Oligodeoxynucleotides that Induce Solvatochromic Fluorescent Lactam by Lysine Modification2019

    • Author(s)
      M. Aso, A. Kinjo, Y. Abe, Y.Taniguchi, S. Sasaki
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Synthesis of Oligodeoxynucleotides for Lysine Modification to Induce Solvatochromic Fluorescent Lactam2018

    • Author(s)
      M. Aso, C. Gatanaga, C. Ota, G. Hirai, Y.Taniguchi, S. Sasaki
    • Organizer
      45th International symposium on nucleic acids chemistry
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2024-01-30  

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