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Development of DNA-based biosensors using evanescent fields

Research Project

Project/Area Number 18K05322
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37010:Bio-related chemistry
Research InstitutionUniversity of Hyogo

Principal Investigator

Yamana Kazushige  兵庫県立大学, 工学研究科, 特任教授 (70192408)

Co-Investigator(Kenkyū-buntansha) 高田 忠雄  兵庫県立大学, 工学研究科, 准教授 (60511699)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsDNA / バイオセンサー / 光電流 / 電気化学 / 医療診断 / プラズモニクス / 近接場光 / 分子認識 / 金属ナノ粒子
Outline of Final Research Achievements

By utilizing duplex formation of DNA, we have prepared gold electrodes modified with gold nanoparticles and developed a photocurrent generation system using near-field light. Perylene diimide (PDI) was introduced between the DNA linking the electrode and the gold nanoparticles, and the photoelectrochemical response generated by irradiating light corresponding to plasmon absorption was observed. We found that the presence of gold nanoparticles significantly increases the photocurrent intensity and that the degree of enhancement depends on the position of the PDI. The mechanism of photocurrent amplification by gold nanoparticles is expected to be useful for the development of DNA-based photobiosensors.

Academic Significance and Societal Importance of the Research Achievements

医療診断への応用を目的として、核酸(DNA, RNA)の分子認識機能を利用したバイオセンサーの開発が広く行われている。本研究では、近接場光を利用した光電流応答型の電気化学DNAセンサーの開発を行った。金ナノ粒子の光励起によって生じる近接場光によって光電流が著しく増強されることを実証した。今後、これらの結果は、光バイオセンサーの開発・性能向上に大きく寄与すると期待できる。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (6 results)

All 2021 2019 2018

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Stacked Thiazole Orange Dyes in DNA Capable of Switching Emissive Behavior in Response to Structural Transitions2021

    • Author(s)
      Takada Tadao、Nishida Koma、Honda Yurika、Nakano Aoi、Nakamura Mitsunobu、Fan Shuya、Kawai Kiyohiko、Fujitsuka Mamoru、Yamana Kazushige
    • Journal Title

      ChemBioChem

      Volume: 22 Issue: 17 Pages: 2729-2735

    • DOI

      10.1002/cbic.202100309

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photocurrent enhancement by a local electric field on DNA-modified electrodes covered with gold nanoparticles2019

    • Author(s)
      Takada Tadao、Syunori Kazue、Nakamura Mitsunobu、Yamana Kazushige
    • Journal Title

      The Analyst

      Volume: 144 Issue: 21 Pages: 6193-6196

    • DOI

      10.1039/c9an01352k

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] Enzymatic preparation of fluorescent DNA functionalized with perylenediimide derivatives2021

    • Author(s)
      Yuuna Yamamoto, Tadao Takada, Ami Takata, Mitsunobu Nakamura, Kazushige Yamana
    • Organizer
      ISNAC2021
    • Related Report
      2021 Annual Research Report
  • [Presentation] シアニン色素を構造内部に有する発光性核酸プローブの開発2019

    • Author(s)
      西田航磨・高田忠雄・本多由理佳・中村光伸・山名一成
    • Organizer
      日本化学会第99春季年会2019
    • Related Report
      2018 Research-status Report
  • [Presentation] “Signal-On”型電気化学核酸検出プローブの開発2019

    • Author(s)
      西村弘基・高田忠雄・山下智也・中村光伸・山名一成
    • Organizer
      日本化学会第99春季年会2019
    • Related Report
      2018 Research-status Report
  • [Presentation] "Signal-on electrochemical sensors utilizing pillar electrodes modified with nucleic acid redox probes"2018

    • Author(s)
      Hiroki Nishimura, Tadao Takada, Tomoya Yamashita, Mitsunobu Nakamura, Kazushige Yamana
    • Organizer
      ISNAC2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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