Structural analysis of a novel S-linked sugar transferase
| Project/Area Number |
18K05340
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
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| Research Institution | Gakushuin University |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 抗生物質 / ペプチド / 糖転移酵素 / 構造生物学 / 結晶構造解析 / X線結晶構造解析 / 糖転移反応 / S-結合型 |
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| Outline of Final Research Achievements |
Protein glycosylation is one of the most common post-translational modifications. N-linked and O-linked glycosylation are the most abundant types, in which sugar is attached to the side chain of asparagine and serine/threonine residues, respectively. In addition, S-linked glycosylation on cysteine residues has been recently known. In this study, a bacterial S-linked glycosyltransferase involved in biosynthesis of a peptide antibiotic was focused on for the purpose of understanding the reaction mechanism of S-glycosylation by the glycosyltransferase, and the crystal structure of the enzyme was successfully determined.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、S-結合型糖転移酵素の立体構造が明らかとなり、他の糖転移酵素とは異なる構造的特徴も見出された。S-結合型糖は、構造的に類似したO-結合型糖よりも加水分解されにくいという特徴があるため、本研究成果により得られた知見をもとに、ペプチド性抗生物質へのS-結合型糖付加による新規抗生物質の開発や、チオール含有化合物への糖付加による物性変化といった研究に資するS-結合型糖転移酵素の酵素改変へと繋がることが期待される。
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Report
(4 results)
Research Products
(6 results)
