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Study on synthetic glycans complexed with glycosidase foe NMR analysis of the active site

Research Project

Project/Area Number 18K05345
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Ishiwata Akihiro  国立研究開発法人理化学研究所, 開拓研究本部, 専任研究員 (70342748)

Co-Investigator(Kenkyū-buntansha) 張 恵平  国立研究開発法人理化学研究所, 放射光科学研究センター, 研究員 (10462706)
Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords合成糖鎖 / 酵素 / 糖鎖-タンパク質複合体 / NMR / 相互作用解析 / 有機合成化学 / 生体分子 / 構造解析 / 糖鎖 / NMR 相互作用解析
Outline of Final Research Achievements

The project entitled “Study on synthetic glycans complexed with glycosidase foe NMR analysis of the active site“ focused on a) chemical synthesis of enzyme substrate glycans and b) analysis using analytical methods. Preparation of a complex arabinan fragment substrate, structural analysis of the obtained substrate in solution state without enzyme, and estimation of the cleavage site specificity have been conducted. It was considered difficult to evaluate the detailed binding motif by NMR method with our system studied this time due to week binding between the glycan-linked probe and the enzyme. The functional analysis of a series of the new arabinan-degrading enzymes, however, could be performed by combining the enzyme reaction monitoring by NMR, the analysis of binding of the ligand probes to active site and novel carbohydrate binding motif from the crystal structure of the substrate-enzyme complex, and the structural analysis of the unexpected product.

Academic Significance and Societal Importance of the Research Achievements

結核菌類細胞壁糖鎖成分の均一な糖鎖やプローブ類をデザインし合成することにより、新規酵素との分子レベルでの相互作用解析にを駆使しより詳細な機能解明を達成する手法を示すことができた。近年遺伝子やアミノ酸シークエンス検索により著しく加速してきている新規酵素探索にその機能解析を組み合わせたブレイクスルーとなると考えている。ミコバクテリアのAGPやLAMの再利用に関する分子レベルでの研究やアラビナン-酵素に関する構造解析等の報告例はごくわずかであり、新たな薬剤の開発への糸口を示した。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (103 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (9 results) Journal Article (31 results) (of which Int'l Joint Research: 12 results,  Peer Reviewed: 30 results,  Open Access: 9 results) Presentation (56 results) (of which Int'l Joint Research: 16 results,  Invited: 8 results) Book (1 results) Remarks (5 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Sun Yat-sen University(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Sun Yat-sen University(中国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Nanyang Technological University(シンガポール)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] The University of York(英国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Leiden University(オランダ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Universitat de Barcelona(スペイン)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Jinan University/Chinese Academy of Sciences/Sun Yat-sen University(中国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Cawthron Institute(ニュージーランド)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research]

    • Related Report
      2020 Research-status Report
  • [Journal Article] Bifidobacterial GH146 β‐ <scp>l</scp> ‐Arabinofuranosidase (Bll4HypBA1) as the Last Enzyme for the Complete Removal of Oligoarabinofuranosides from Hydroxyproline‐Rich Glycoproteins2023

    • Author(s)
      Ishiwata Akihiro、Tsunomachi Hanako、Kameyama Kyohei、Sophon Kaeothip、Nakamura Masayuki、Kitahara Kanefumi、Tanaka Katsunori、Ito Yukishige、Fujita Kiyotaka
    • Journal Title

      ChemBioChem

      Volume: 24 Issue: 5

    • DOI

      10.1002/cbic.202200637

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Insight into an Enzyme that Breaks Down Oligosaccharides in Caramel2022

    • Author(s)
      KASHIMA Toma、FUSHINOBU Shinya、ISHIWATA Akihiro、FUJITA Kiyotaka
    • Journal Title

      Nihon Kessho Gakkaishi

      Volume: 64 Issue: 4 Pages: 263-264

    • DOI

      10.5940/jcrsj.64.263

    • ISSN
      0369-4585, 1884-5576
    • Year and Date
      2022-12-15
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and synthesis of the mechanism-based inhibitors against the GH127/146 cysteine glycosidase2022

    • Author(s)
      Ishiwata Akihiro, Tanaka Katsunori、Fujita Kiyotaka、Ito Yukishige
    • Journal Title

      Peptide Science

      Volume: 2022 Pages: 65-68

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ZnI<sub>2</sub>-Mediated β-Galactosylation of C2-Ether-Type Donor2022

    • Author(s)
      Zhou Siai、Ao Jiaming、Guo Aoxin、Zhao Xiaoya、Deng Nan、Wang Guoqing、Yang Qixuan、Ishiwata Akihiro、Liu Xue-Wei、Li Qianqian、Cai Hui、Ding Feiqing
    • Journal Title

      Organic Letters

      Volume: 24 Issue: 43 Pages: 8025-8030

    • DOI

      10.1021/acs.orglett.2c03256

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mechanism-based inhibition of GH127/146 cysteine glycosidases by stereospecifically functionalized l-arabinofuranosides2022

    • Author(s)
      Ishiwata Akihiro、Narita Satoru、Kimura Kenta、Tanaka Katsunori、Fujita Kiyotaka、Fushinobu Shinya、Ito Yukishige
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 75 Pages: 117054-117054

    • DOI

      10.1016/j.bmc.2022.117054

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An experimental and theoretical study on stereocontrolled glycosylations by a “one-pot” procedure2022

    • Author(s)
      Zhong Xuemei、Zhao Xiaoya、Ao Jiaming、Huang Yan、Liu Yuhua、Zhou Siai、Li Bizhi、Ishiwata Akihiro、Fang Qianglin、Yang Chongguang、Cai Hui、Ding Feiqing
    • Journal Title

      Organic Chemistry Frontiers

      Volume: 9 Issue: 15 Pages: 4151-4157

    • DOI

      10.1039/d2qo00727d

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Synthesis of naturally occurring β-l-arabinofuranosyl-l-arabinofuranoside structures towards the substrate specificity evaluation of β-l-arabinofuranosidase2022

    • Author(s)
      Ishiwata Akihiro、Fujita Kiyotaka、Fushinobu Shinya、Tanaka Katsunori、Ito Yukishige
    • Journal Title

      Bioorganic & Medicinal Chemistry

      Volume: 68 Pages: 116849-116849

    • DOI

      10.1016/j.bmc.2022.116849

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes2022

    • Author(s)
      Sasaki Yuki、Komeno Masahiro、Ishiwata Akihiro、Horigome Ayako、Odamaki Toshitaka、Xiao Jin-Zhong、Tanaka Katsunori、Ito Yukishige、Kitahara Kanefumi、Ashida Hisashi、Fujita Kiyotaka
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 88 Issue: 6

    • DOI

      10.1128/aem.02187-21

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent advances in stereoselective 1,2-cis-O-glycosylations2022

    • Author(s)
      Ishiwata Akihiro、Tanaka Katsunori、Ao Jiaming、Ding Feiqing、Ito Yukishige
    • Journal Title

      Frontiers in Chemistry

      Volume: 10 Pages: 972429-972429

    • DOI

      10.3389/fchem.2022.972429

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recent chemical and chemoenzymatic strategies to complex-type N-glycans.2022

    • Author(s)
      79.Ding, F.; Zhao, X.; Huang, Y.; Zhou, A.; Ao, J.; Cai, H.; Tanaka, K., Ito, Y., Ishiwata, A.
    • Journal Title

      Frontiers in Chemistry

      Volume: - Pages: 880128-880128

    • DOI

      10.3389/fchem.2022.880128

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification and Structural Basis of an Enzyme Degrading Oligosaccharides in Caramel2022

    • Author(s)
      KASHIMA Toma、ISHIWATA Akihiro、FUJITA Kiyotaka、FUSHINOBU Shinya
    • Journal Title

      Seibutsu Butsuri

      Volume: 62 Issue: 3 Pages: 184-186

    • DOI

      10.2142/biophys.62.184

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent advances of the stereoselective bimodal glycosylations for the synthesis of various glucans2022

    • Author(s)
      Ding Feiqing、Ishiwata Akihiro、Ito Yukishige
    • Journal Title

      Studies in Natural Products Chemistry (Bioactive Natural Products)

      Volume: 74 Pages: 1-40

    • DOI

      10.1016/b978-0-323-91099-6.00001-3

    • ISBN
      9780323910996
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Substrate complex structure, active site labeling and catalytic role of the zinc ion in cysteine glycosidase2021

    • Author(s)
      Maruyama Shun、Sawano Kota、Amaki Satoko、Suzuki Takehiro、Narita Satoru、Kimura Kenta、Arakawa Takatoshi、Yamada Chihaya、Ito Yukishige、Dohmae Naoshi、Fujita Kiyotaka、Ishiwata Akihiro、Fushinobu Shinya
    • Journal Title

      Glycobiology

      Volume: 32 Issue: 2 Pages: 171-180

    • DOI

      10.1093/glycob/cwab103

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of difructose dianhydride I synthase/hydrolase from an oral bacterium establishes a novel glycoside hydrolase family2021

    • Author(s)
      Kashima Toma、Okumura Kouki、Ishiwata Akihiro、Kaieda Machika、Terada Tohru、Arakawa Takatoshi、Yamada Chihaya、Shimizu Kentaro、Tanaka Katsunori、Kitaoka Motomitsu、Ito Yukishige、Fujita Kiyotaka、Fushinobu Shinya
    • Journal Title

      Journal of Biological Chemistry

      Volume: 297 Issue: 5 Pages: 101324-101324

    • DOI

      10.1016/j.jbc.2021.101324

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zinc(II) Iodide-Directed β-Mannosylation: Reaction Selectivity, Mode, and Application2021

    • Author(s)
      Zhong Xuemei、Zhou Siai、Ao Jiaming、Guo Aoxin、Xiao Qian、Huang Yan、Zhu Wanmeng、Cai Hui、Ishiwata Akihiro、Ito Yukishige、Liu Xue-Wei、Ding Feiqing
    • Journal Title

      The Journal of Organic Chemistry

      Volume: 86 Issue: 23 Pages: 16901-16915

    • DOI

      10.1021/acs.joc.1c02091

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of a novel 3-O-α-D-galactosyl-α-L-arabinofuranosidase for the assimilation of gum arabic AGP in Bifidobacterium longum subsp. longum2021

    • Author(s)
      Sasaki Yuki、Horigome Ayako、Odamaki Toshitaka、Xiao Jin-Zhong、Ishiwata Akihiro、Ito Yukishige、Kitahara Kanefumi、Fujita Kiyotaka
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 87 Issue: 10 Pages: 1-20

    • DOI

      10.1128/aem.02690-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cysteine Nucleophiles in Glycosidase Catalysis: Application of a Covalent β‐ l‐ Arabinofuranosidase Inhibitor2021

    • Author(s)
      McGregor Nicholas G. S.、Coines Joan、Borlandelli Valentina、Amaki Satoko、Artola Marta、Nin‐Hill Alba、Linzel Daniel、Yamada Chihaya、Arakawa Takatoshi、Ishiwata Akihiro、Ito Yukishige、Marel Gijsbert A.、Codee Jeroen D. C.、Fushinobu Shinya、Overkleeft Herman S.、Rovira Carme、Davies Gideon J..
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 60 Issue: 11 Pages: 5754-5758

    • DOI

      10.1002/anie.202013920

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory effects of sesquiterpene lactones from the Indonesian marine sponge Lamellodysidea cf. herbacea on bone morphogenetic protein-induced osteoblastic differentiation2021

    • Author(s)
      Ohte Satoshi、Yamazaki Hiroyuki、Takahashi Ohgi、Rotinsulu Henki、Wewengkang Defny S.、Sumilat Deiske A.、Abdjul Delfly B.、Maarisit Wilmar、Kapojos Magie M.、Zhang Huiping、Hayashi Fumiaki、Namikoshi Michio、Katagiri Takenobu、Tomoda Hiroshi、Uchida Ryuji
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 35 Pages: 127783-127783

    • DOI

      10.1016/j.bmcl.2021.127783

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Brevisulcenals-A1 and A2, Sulfate Esters of Brevisulcenals, Isolated from the Red Tide Dinoflagellate Karenia brevisulcata2021

    • Author(s)
      Satake Masayuki、Irie Raku、Holland Patrick T.、Harwood D Tim、Shi Feng、Itoh Yoshiyuki、Hayashi Fumiaki、Zhang Huiping
    • Journal Title

      Toxins

      Volume: 13 Issue: 2 Pages: 82-82

    • DOI

      10.3390/toxins13020082

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibition of cholesteryl ester synthesis by polyacetylenes from Atractylodes rhizome.2020

    • Author(s)
      Nur EAA, Ohshiro T, Kobayashi K, Wu J, Wahyudin E, Zhang H, Hayashi F, Kawagishi H, Tomoda H.
    • Journal Title

      Bioorg Med Chem Lett

      Volume: 30 Issue: 7 Pages: 126997-126997

    • DOI

      10.1016/j.bmcl.2020.126997

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 4-Hydroxy Pyridones from Heterologous Expression and Cultivation of the Native Host2020

    • Author(s)
      Zhang Wei-Yang、Zhong Yue、Yu Yang、Shi Dan-Feng、Huang Hui-Yun、Tang Xiao-Long、Wang Yong-Heng、Chen Guo-Dong、Zhang Hui-Ping、Liu Chen-Li、Hu Dan、Gao Hao、Yao Xin-Sheng
    • Journal Title

      Journal of Natural Products

      Volume: 83 Issue: 11 Pages: 3338-3346

    • DOI

      10.1021/acs.jnatprod.0c00675

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unified Strategy toward Stereocontrolled Assembly of Various Glucans Based on Bimodal Glycosyl Donors2020

    • Author(s)
      Ding Feiqing、Ishiwata Akihiro、Zhou Siai、Zhong Xuemei、Ito Yukishige
    • Journal Title

      The Journal of Organic Chemistry

      Volume: 85 Issue: 8 Pages: 5536-5558

    • DOI

      10.1021/acs.joc.0c00292

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 糖質関連酵素の基質としての複合糖質天然物の合成2020

    • Author(s)
      石渡明弘
    • Journal Title

      化学工業

      Volume: 71

    • Related Report
      2019 Research-status Report
  • [Journal Article] Beijinchromes A–D, Novel Aromatic Compounds Isolated from <i>Nocardia beijingensis</i> NBRC 163422019

    • Author(s)
      Hoshino Shotaro、Awakawa Takayoshi、Zhang Huiping、Hayashi Fumiaki、Abe Ikuro
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 67 Issue: 8 Pages: 775-777

    • DOI

      10.1248/cpb.c19-00364

    • NAID

      130007686331

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2019-08-01
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Synthetic Study on Glycoconjugates Containing 1,2-<i>cis</i> Glycoside and Their Application2019

    • Author(s)
      Ishiwata Akihiro
    • Journal Title

      Trends in Glycoscience and Glycotechnology

      Volume: 31 Issue: 181 Pages: SE53-SE54

    • DOI

      10.4052/tigg.1925.2SE

    • NAID

      130007683625

    • ISSN
      0915-7352, 1883-2113
    • Year and Date
      2019-07-25
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Discovery of Fungal Polyene Macrolides via a Postgenomic Approach Reveals a Polyketide Macrocyclization by trans-Acting Thioesterase in Fungi2019

    • Author(s)
      Morishita Yohei、Zhang Huiping、Taniguchi Tohru、Mori Keiji、Asai Teigo
    • Journal Title

      Organic Letters

      Volume: 21 Issue: 12 Pages: 4788-4792

    • DOI

      10.1021/acs.orglett.9b01674

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mirilactams C–E, Novel Polycyclic Macrolactams Isolated from Combined-Culture of <i>Actinosynnema mirum</i> NBRC 14064 and Mycolic Acid-Containing Bacterium2018

    • Author(s)
      Hoshino Shotaro、Ozeki Masahiro、Wong Chin Piow、Zhang Huiping、Hayashi Fumiaki、Awakawa Takayoshi、Morita Hiroyuki、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 66 Issue: 6 Pages: 660-667

    • DOI

      10.1248/cpb.c18-00143

    • NAID

      130007382327

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2018-06-01
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stereodivergent Mannosylation Using 2-O-(ortho-Tosylamido)benzyl Group2018

    • Author(s)
      Ding Feiqing、Ishiwata Akihiro、Ito Yukishige
    • Journal Title

      Organic Letters

      Volume: 20 Issue: 16 Pages: 4833-4837

    • DOI

      10.1021/acs.orglett.8b01979

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bimodal Glycosyl Donors Protected by 2-O-(ortho-Tosylamido)benzyl Group2018

    • Author(s)
      Ding Feiqing、Ishiwata Akihiro、Ito Yukishige
    • Journal Title

      Organic Letters

      Volume: 20 Issue: 14 Pages: 4384-4388

    • DOI

      10.1021/acs.orglett.8b01922

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Umezawamides, new bioactive polycyclic tetramate macrolactams isolated from a combined-culture of Umezawaea sp. and mycolic acid-containing bacterium2018

    • Author(s)
      Hoshino Shotaro、Wong Chin Piow、Ozeki Masahiro、Zhang Huiping、Hayashi Fumiaki、Awakawa Takayoshi、Asamizu Shumpei、Onaka Hiroyasu、Abe Ikuro
    • Journal Title

      The Journal of Antibiotics

      Volume: 71 Issue: 7 Pages: 653-657

    • DOI

      10.1038/s41429-018-0040-4

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hinduchelins A-D, Noncytotoxic Catechol Derivatives from <i>Streptoalloteichus hindustanus</i>2018

    • Author(s)
      He Fei、Nakamura Hitomi、Hoshino Shotaro、Fong Chin Joyce Seow、Yang Liang、Zhang Huiping、Hayashi Fumiaki、Abe Ikuro
    • Journal Title

      Journal of Natural Products

      Volume: 81 Issue: 6 Pages: 1493-1496

    • DOI

      10.1021/acs.jnatprod.8b00049

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Chemical Approaches toward Understanding Cysteine Glycosidase Catalysis2023

    • Author(s)
      Ishiwata Akihiro
    • Organizer
      Seventh International Conference on Catalysis and Chemical Engineering,
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] exo-β-D-arabinofuranosidase のD-アラビノフラノシド含有阻害剤プローブの合成2023

    • Author(s)
      石渡明弘, 藤田清貴, 田中克典, 伊藤幸成
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] GH127/146 β-L-アラビノフラノシダーゼ阻害剤の合成と阻害機構解析2023

    • Author(s)
      石渡明弘, 藤田清貴, 伏信進矢, 田中克典, 伊藤幸成
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Synthesis and structural analysis of D-fructofuranosylated compounds for the analysis of GH172 difructose dianhydride I synthase/hydrolase2022

    • Author(s)
      Ishiwata Akihiro, Toma Kashima, Machika Kaieda, Katsunori Tanaka, Kiyotaka Fujita, Shinya Fushinobu, Yukishige Ito
    • Organizer
      30th International Carbohydrate Symposium
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design and synthesis of the mechanism-based inhibitors against the glycoside hydrolase family 127/146 cysteine glycosidase2022

    • Author(s)
      Akihiro Ishiwata, Katsunori Tanaka, Kiyotaka Fujita, Yukishige Ito
    • Organizer
      第59回ペプチド討論会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ビフィズス菌由来AGP分解酵素群の機能解析へ向けた構造解析研究2022

    • Author(s)
      石渡明弘, 佐々木優紀, 藤田清貴, 北原兼文, 伏信進矢, 田中克典, 伊藤幸成
    • Organizer
      第41回日本糖質学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] β-D-アラビノフラノシド含有プローブの立体選択的合成とβ-D-arabinofuranosidaseの機能解析2022

    • Author(s)
      石渡明弘, 藤田清貴, 田中克典, 伊藤幸成
    • Organizer
      日本応用糖質科学会2022年度大会 (第71回)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Bacteroides caccae 由来のGH91イヌリン分解酵素のクローニングと性質解析2022

    • Author(s)
      志手由里奈, 石渡明弘, 伊藤幸成, 北原兼文, 藤田清貴
    • Organizer
      日本応用糖質科学会2022年度大会 (第71回)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Structural basis of glycoside hydrolases for α- and β-D-arabinofuranosidic bonds of mycobacterial lipoarabinomannan2022

    • Author(s)
      Shinya Fushinobu, Chiho Nakashima, Jiaman Li, Riku Fukushima, Akihiro Ishiwata, Toma Kashima, Miki Sawai, Michiko Shimokawa, Sae Morigami, Kiyotaka Fujita
    • Organizer
      14th Carbohydrate Bioengineering Meeting (CBM)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Synthesis of L-arabinofuranoside derivatives for the analysis of novel β-L-arabinofuranosidases and its homologues2022

    • Author(s)
      Akihiro Ishiwata
    • Organizer
      Asian Carbohydrate Chemistry and Glycobiology Webinar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Bacteroides ovatusのイヌリン分解におけるジアンヒドリド構造含有生成物の解析,2022

    • Author(s)
      石渡明弘, 志手由里奈, 北原兼文, 田中克典, 藤田清貴, 伊藤幸成
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Research-status Report
  • [Presentation] β-D-アラビノフラノシド含有プローブの合成と新規exo-β-D-arabinofuranosidase の機能解析2022

    • Author(s)
      石渡明弘, 藤田清貴, 田中克典, 伊藤幸成
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] アラビノフラノシド誘導体の化学合成とアラビナン分解酵素解析への応用2021

    • Author(s)
      石渡明弘
    • Organizer
      第18回糖鎖科学コンソーシアムシンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Synthesis and structural analysis of glycans and glycoconjugates as the substrate for glycan-related bacterial enzymes2021

    • Author(s)
      Akihiro Ishiwata
    • Organizer
      13rd Glyco-lipidologue seminar
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Synthetic study of D-arabino- and D-fructo-furanosylated probes for mycobacterial arabinan degrading enzymes and its homologues2021

    • Author(s)
      Akihiro Ishiwata, Yukishige Ito
    • Organizer
      PACIFICHEM 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bifidobacterium longum の菌株特異的なアラビアガム分解メカニズムの全容解明2021

    • Author(s)
      佐々木優紀, 芦田久, 石渡明弘, 伊藤幸成, 堀米綾子, 小田巻俊孝, 清水(肖)金忠, 北原兼文, 藤田清貴
    • Organizer
      日本乳酸菌学会2021年度大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ビフィズス菌のアラビアガム資化性の鍵を握る3-O-α-D-galactopyranosyl-α-L-arabinofuranosidaseの機能解析2021

    • Author(s)
      佐々木優紀, 堀米綾子, 小田巻俊孝, 清水(肖)金忠, 石渡明弘, 伊藤幸成, 北原兼文, 藤田清貴
    • Organizer
      日本応用糖質科学会2021年度大会 (第70回) 「応用糖質科学シンポジウム」
    • Related Report
      2021 Research-status Report
  • [Presentation] Bifidobacterium dentiumにおけるカラメル化糖の分解メカニズムの解析2021

    • Author(s)
      海江田麿千加, 奥村公喜, 鹿島騰真, 伏信進矢, 石渡明弘, 伊藤幸成, 北原兼文, 藤田清貴
    • Organizer
      日本応用糖質科学会2021年度大会 (第70回)
    • Related Report
      2021 Research-status Report
  • [Presentation] Bacteroides ovatusの培養上清中に蓄積するイヌリン分解産物の構造解析2021

    • Author(s)
      志手由里奈, 石渡明弘, 伊藤幸成, 北原兼文, 藤田清貴
    • Organizer
      日本農芸化学会2021年度西日本・中四国・関西支部 合同大会
    • Related Report
      2021 Research-status Report
  • [Presentation] ビフィズス菌由来新規exo-α-D-fructofuranosidaseホモログの反応機構研究2021

    • Author(s)
      石渡明弘, 藤田清貴, 鹿島騰真, 伏信進矢, 田中克典, 伊藤幸成
    • Organizer
      第40回日本糖質学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] D-アラビナン分解酵素ホモローグ解析用フラノシド含有プローブの合成研究2021

    • Author(s)
      石渡明弘, 藤田清貴, 伊藤幸成
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Research-status Report
  • [Presentation] ビフィズス菌由来新規exo-α-D-fructofuranosidaseの機構解析用フラノシド誘導体の合成研究2021

    • Author(s)
      石渡明弘, 鹿島騰真, 藤田清貴, 伏信進矢, 伊藤幸成
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 阻害剤を用いたβ-L-アラビノフラノシダーゼの研究2021

    • Author(s)
      味木智子, 成田覚, 鈴木健裕, 澤野孝太, 荒川孝俊, 山田千早, 藤田清貴, 伊藤幸成, 堂前直, 石渡明弘
    • Organizer
      2020年度量子ビームサイエンスフェスタ
    • Related Report
      2020 Research-status Report
  • [Presentation] 複合糖質の化学合成~微生物や植物の糖関連酵素を化学的に探る~2020

    • Author(s)
      石渡明弘
    • Organizer
      群馬大学-前橋工科大学合同中間報告会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 阻害剤を用いた GH127 β-L-アラビノフラノシダーゼの反応機構に関する研究2020

    • Author(s)
      味木智子, 成田覚, 鈴木健裕, 澤野孝太, 荒川孝俊, 山田千早, 藤田清貴, 伊藤幸成, 堂前直, 石渡明弘, 伏信進矢
    • Organizer
      日本農芸化学会 関東支部 2020年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] ビフィズス菌由来アラビナン分解酵素ホモローグ解析用フラノシド含有プローブの合成2020

    • Author(s)
      石渡明弘, 藤田清貴, 伊藤幸成
    • Organizer
      第39回日本糖質学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] カラメル化糖に作用するビフィズス菌の新規exo-α-D-fructofuranosidaseの構造解析2020

    • Author(s)
      鹿島騰真, 奥村公喜, 荒川孝俊, 山田千早, 石渡明弘, 伊藤幸成, 藤田清貴, 伏信進矢
    • Organizer
      日本応用糖質科学会2020年度大会 (第69回)
    • Related Report
      2020 Research-status Report
  • [Presentation] エキソ-β-1,3-ガラクタナーゼとβ-L-アラビノフラノシダーゼ遺伝子の異種発現系の構築とガムアラビックに対する反応性2020

    • Author(s)
      大前晴香, 高濱里帆, 中澤昌美, 上田光宏, 石渡明弘, 伊藤幸成, 藤田清貴, 阪本龍司
    • Organizer
      日本応用糖質科学会2020年度大会 (第69回)
    • Related Report
      2020 Research-status Report
  • [Presentation] Structural characterization of a novel exo-α-D-fructofuranosidase from Bifidobacterium dentium that degarades caramelized sugars2020

    • Author(s)
      Kashima Toma, Kouki Okumura, Takatoshi Arakawa, Akihiro Ishiwata, Yukishige Ito, Kiyotaka Fujita, Shinya Fushinobu,
    • Organizer
      第20回日本蛋白質科学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] Structural characterization of a novel exo-α-D-fructofuranosidase from Bifidobacterium dentium that degarades caramelized sugars2020

    • Author(s)
      Toma Kashima, Kouki Okumura, Takatoshi Arakawa, Akihiro Ishiwata, Yukishige Ito, Kiyotaka Fujita, Shinya Fushinobu
    • Organizer
      2020 World Conference on Protein Science
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Presentation] アラビナン分解酵素の機能解析に向けたD-アラビノフラノシド含有プローブの合成研究2020

    • Author(s)
      石渡明弘, 伊藤幸成
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Synthesis of L-arabinofuranosylamine derivatives for the analysis of novel β-L-arabinofuranosidases from Bifidobacterium longum2019

    • Author(s)
      Ishiwata A.; Narita, S.; Ito Y.
    • Organizer
      11th Asian Community of Glycoscience and Glycotechology Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of mycobacterial arabinan degrading enzymes from Microbacterium arabinogalactanolyticum2019

    • Author(s)
      Fujita K.; Ishiwata, A.; Fushinobu S.; Suda, Y.; Ito, Y.; Kitahara,
    • Organizer
      258th American Chemical Society meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural analysis for studying the reaction mechanism of β-L-arabinofuranosidases2019

    • Author(s)
      Fushinobu S.; Maruyama, S.; Sawano, K.; Arakawa, T.; Yamada, C.; Ishiwata, A.; Ito, Y.; Fujita K.
    • Organizer
      258th American Chemical Society meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Synthesis of Oligo-D-arabinofuranosylated probes as the substrate for mycobacterial arabinan degrading enzymes2019

    • Author(s)
      Ishiwata, A.; Fushinobu S.; Fujita K.; Ito, Y.
    • Organizer
      258th American Chemical Society meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Synthesis of D-arabinofuranosylated probes towards the functional analysis of mycobacterial arabinan degrading enzymes2019

    • Author(s)
      Ishiwata, A.; Fushinobu S.; Fujita K.; Ito, Y.
    • Organizer
      EUROCARBO 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] β-L-アラビノフラノシダーゼ阻害剤の合成と構造解析2019

    • Author(s)
      石渡明弘, 成田覚, 伊藤幸成
    • Organizer
      日本化学会第100春季年会
    • Related Report
      2019 Research-status Report
  • [Presentation] D-アラビノフラノシダーゼの機能解析へ向けたD-アラビナン部分構造の合成検討2019

    • Author(s)
      石渡明弘, 伊藤幸成
    • Organizer
      日本応用糖質科学会2019年度大会 (第68回)
    • Related Report
      2019 Research-status Report
  • [Presentation] D-アラビナン分解酵素群の機能解析へ向けたD-アラビナンフラグメントおよびプローブの合成2019

    • Author(s)
      石渡明弘, 伊藤幸成
    • Organizer
      第38回日本糖質学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 結核菌アラビナン分解酵素群の機能解析へ向けたアラビナンフラグメントの合成研究2019

    • Author(s)
      石渡明弘, 伊藤幸成
    • Organizer
      第115回有機合成シンポジウム2019年
    • Related Report
      2019 Research-status Report
  • [Presentation] Synthesis of the arabinan fragments of mycobacterial cell wall2018

    • Author(s)
      ○Ishiwata, A.; Ito, Y.
    • Organizer
      9th Asian Community of Glycoscience and Glycotechology Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Strategy toward the synthesis of glycoconjugate from plant and bacteria2018

    • Author(s)
      ○Ishiwata, A.
    • Organizer
      ACS on Campus in AIST
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] L-Arabinofuranosylated probes for the analysis of novel L-arabinofuranosidases from Bifidobacterium longum2018

    • Author(s)
      ○Ishiwata, A.; Ito, Y.
    • Organizer
      29th International Carbohydrate Symposium,
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Synthesis of arabinan fragments from mycobacterial call wall component towards the functional analysis of mycobacterial arabinan degrading enzymes2018

    • Author(s)
      ○Akihiro Ishiwata, Yukishige Ito
    • Organizer
      日本化学会第99春季年会,
    • Related Report
      2018 Research-status Report
  • [Presentation] GH127 -L-アラビノフラノシダーゼ阻害剤の合成検討2018

    • Author(s)
      ○石渡明弘, 伊藤幸成,
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Microbacterium arabinogalactanolyticum由来の組み換えendo-D-arabinaseの機能解析2018

    • Author(s)
      ○藤田清貴, 田中悠暉, 石渡明弘, 伊藤幸成, 隅田泰生, 北原兼文
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 〇澤野孝太, 成田覚, 荒川孝俊, 山田千早, 藤田清貴, 石渡明弘, 伊藤幸成, 伏信進矢2018

    • Author(s)
      GH127β-l-アラビノフラノシダーゼと阻害剤の複合体構造
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ビフィズス菌由来 -アラビノフラノシダーゼの基質及び阻害剤の立体選択的合成研究2018

    • Author(s)
      ○石渡明弘, 成田覚, 伊藤幸成,
    • Organizer
      GlycoTokyo 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] ビフィズス菌由来アラビアガム分解酵素3-O--D-galactosyl--L-arabinofuranosidaseの機能解析2018

    • Author(s)
      〇佐々木優紀, 堀米綾子, 小田巻俊孝, 清水(肖)金忠, 石渡明弘,伊藤幸成,北原兼文, 藤田清貴
    • Organizer
      生物工学会, 九州支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] カルボリン誘導体の小胞体グルコシダーゼII阻害による抗ウイルス活性2018

    • Author(s)
      ○恩田桃子, 石渡明弘, 袴田航, 平野貴子, 伊藤幸成, 遠矢幸伸, 西尾俊幸
    • Organizer
      日本農芸化学会関東支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Stereodivergent Mannosylation Using 2-O-(ortho-Tosyl- amido)benzyl Group2018

    • Author(s)
      ○Ding F. Q.; Ishiwata A.; Ito Y.
    • Organizer
      第5回 FCCA シンポジウム・グライコサイエンス若手フォーラム 2018
    • Related Report
      2018 Research-status Report
  • [Presentation] Bifidobacterium longum由来の-L-アラビノフラノシダーゼの機構解析に向けたアラビノフラノース構造を有するプローブの合成検討2018

    • Author(s)
      ○石渡明弘, 成田覚, 藤田清貴, 伏信 進矢, 伊藤幸成,
    • Organizer
      第37回日本糖質学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒト培養細胞におけるカルボリン誘導体の小胞体グルコシダーゼII 阻害:網羅的 N-結合型糖鎖解析による作用標的の検討2018

    • Author(s)
      ○恩田桃子, 石渡明弘, 伊藤幸成, 氏家誠, 袴田航, 平野貴子, 西尾俊幸.
    • Organizer
      日本ケミカルバイオロジー学会 第13回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] “Open advanced research facilities/NMR platform initiative” program in RIKEN2018

    • Author(s)
      ○Huiping Zhang
    • Organizer
      2nd China-Japan Joint Symposium on Natural Product Biosynthesis
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] NMR, LC/MS を用いた大黄の多成分評価2018

    • Author(s)
      ○小栗志織, 中村理恵, 平倉一弘, 青木勝之, 橋本和則, 高橋豊, 張恵平, 林文晶, 前田秀明
    • Organizer
      日本生薬学会第65回年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 糸状菌マクロライド化合物のポストゲノム型天然物探索2018

    • Author(s)
      ○下陽平, 伊藤芽衣, 青木優, 浜口昌武, 張恵平, 清水公徳, 浅井禎吾
    • Organizer
      第60回天然有機化合物討論会
    • Related Report
      2018 Research-status Report
  • [Book] Recent advances of the stereoselective bimodal glycosylations for the synthesis of various glucans, Ding Feiqing,2022

    • Author(s)
      Recent advances of the stereoselective bimodal glycosylations for the synthesis of various glucans
    • Publisher
      Elsevier
    • ISBN
      9780323910996
    • Related Report
      2021 Research-status Report
  • [Remarks] 植物糖タンパク質に残された最後の糖を切断する酵素をビフィズス菌から発見

    • URL

      https://www.kagoshima-u.ac.jp/topics/2023/01/post-2009.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] システインが触媒の中心的役割を担う糖質分解酵素のメカニズムを解明

    • URL

      https://www.a.u-tokyo.ac.jp/topics/topics_20210203-1.html

    • Related Report
      2020 Research-status Report
  • [Remarks] ビフィズス菌がアラビアガムを利用して増殖するメカニズムを解明

    • URL

      https://www.kagoshima-u.ac.jp/topics/2021/03/post-1773.html

    • Related Report
      2020 Research-status Report
  • [Remarks] アラビアガムがビフィズス菌を増やす仕組みを解明 ―鹿児島大学との共同研究からー

    • URL

      https://www.morinagamilk.co.jp/release/newsentry-3614.html

    • Related Report
      2020 Research-status Report
  • [Remarks] ビフィズス菌増の酵素発見

    • URL

      https://s1.373news.com/minamiru/_news/minamiNews/?storyid=135632&topicid=220&mediaid=3

    • Related Report
      2020 Research-status Report
  • [Patent(Industrial Property Rights)] 糖質分解酵素、食品添加用細菌のスクリーニング方法、        プレバイオティクスの評価方法及びプライマー対2020

    • Inventor(s)
      藤田清貴、石渡明弘
    • Industrial Property Rights Holder
      藤田清貴、石渡明弘
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-055336
    • Filing Date
      2020
    • Related Report
      2019 Research-status Report

URL: 

Published: 2018-04-23   Modified: 2024-01-30  

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