| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
G-quadruplexes (G4s) are higher-order structures of nucleic acids formed from Hoogsteen base pairs in guanine-rich sequences. G4s regulate a wide variety of biological processes such as stress granule formation. G4-binding small molecules (G4 ligands) have been played a key role in the progress of this field. Notably, light-up G4 ligands whose fluorescence is selectively enhanced when interacting with G4s are useful for high-sensitivity detection. Herein, we present a fluorescent light-up probe with the ability to selectively stabilize G4s and enhance fluorescence upon G4 binding.
The foci of the probe were mainly observed in the nucleoli. These were co-localized with anti-fibrillarin antibodies and anti-G4 antibodies (BG4). Moreover, we tested detection of G4 in stress granules using the developed probe. We present a practical light-up probe for G4s in stress granules, providing potential targets for G4 ligands.
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