| Project/Area Number |
18K05354
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Kobori Akio 京都工芸繊維大学, 分子化学系, 教授 (00397605)
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| Co-Investigator(Kenkyū-buntansha) |
和久 友則 京都工芸繊維大学, 分子化学系, 准教授 (30548699)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 表面増強ラマン散乱 / DNA / RNA / ラマン散乱 / SERS / 核酸 / イメージング / バイオマーカー |
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| Outline of Final Research Achievements |
Quantification of trace amounts of nucleic acids in bio-samples is a promising way for diagnosis of virus-induced diseases. Although qPCR is the gold standard for quantification of nucleic acids, development of facile and accurate methods for diagnosis are quite important. One of the answers are the methods using surface-enhanced Raman scattering, SERS. Sensitivity of SERS is dramatically increased over the last two decades and almost outstripped that of fluorescent measurements. However significant improvements for reproducibility of SERS measurements have been required from a practical application. In this study, we newly designed a ratiometric SERS-based assay of nucleic acids. This assay uses two Raman tags as an internal standard and Raman reporter. By this method, sub-nanomolar of the target DNAs were automatically detected with small standard deviations.
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| Academic Significance and Societal Importance of the Research Achievements |
サイレント領域にラマンシグナルを発するラマンタグを2つ導入したSERSプローブを利開発したことで、簡便かつ高感度な核酸測定系の実現に成功した。本手法における核酸検出感度は、蛍光タグを利用した従来の核酸測定法と同程度であることを示すことに成功している。また、ラマン顕微鏡を利用したマッピング測定にSERS測定系を融合可能であることも実験から証明しているため、ラマン分光測定の新たな可能性を示す結果として大いに評価できる。
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