Lipid Raft Analysis in Virus-Infected Cell Models
Project/Area Number |
18K05356
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 37030:Chemical biology-related
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2018-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | HaloTag / ヘマグルチニン / 合成糖鎖 / インフルエンザウイルス / シアル酸 / 生細胞イメージング / 膜流動性 / 脂質ラフト / 相分離リポソーム / FRAP解析 / ウイルス / 糖鎖 / 蛍光顕微鏡 |
Outline of Final Research Achievements |
In this study, we generated synthetic glycan-presenting cells using HaloTag, which can recombine only cell surface glycans, in order to construct mutation-responsive sensor cells for viruses that mutate in a variety of ways. When these cells were exposed to influenza surface molecules, hemagglutinin (human H1N1 and avian H5N1), differences in the binding ability of cells presenting specific glycan structures were observed by live cell imaging. This achievement is important because it used living cells, which tend to form a patch structure such as lipid rafts, rather than a fixed support substrate. In the future, we would like to increase the sensitivity of the above sensor cells by optimizing the selection of cells and analytical conditions.
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Academic Significance and Societal Importance of the Research Achievements |
本研究で構築を試みたセンサーセルは、変異を繰り返すウイルスに対して宿主細胞の表面に存在する糖鎖パターンを人為的に操作することにより、ウイルスの型を判別することができる仕組みを有する。今回はインフルエンザウイルスに着目したが、同じエンベロープ型のウイルスであるCOVID-19の変異に対しても応用できる可能性がある。
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Report
(5 results)
Research Products
(122 results)
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[Journal Article] Immunological Evaluation of Conjugate/Co-Assembly of Peptide Antigen and Adjuvant as Self-adjuvanting Anti-breast Cancer Vaccine Candidates.2021
Author(s)
Ito K., Manabe Y., Aiga T., Chang TC., Kabayama K., Ohshima S., Kametani Y., Furukawa H., Inaba H., Matsuura K., Fukase K.
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Journal Title
Peptide Science 2020
Volume: 2020
Pages: 19-20
Related Report
Peer Reviewed
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[Journal Article] α‐Emitting cancer therapy using 211 At‐AAMT targeting LAT12021
Author(s)
Kaneda‐Nakashima Kazuko、Zhang ZiJian、Manabe Yoshiyuki、Shimoyama Atsushi、Kabayama Kazuya、Watabe Tadashi、Kanai Yoshikatsu、Ooe Kazuhiro、Toyoshima Atsushi、Shirakami Yoshifumi、Yoshimura Takashi、Fukuda Mitsuhiro、Hatazawa Jun、Nakano Takashi、Fukase Koichi、Shinohara Atsushi
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Journal Title
Cancer Science
Volume: 112
Issue: 3
Pages: 1132-1140
DOI
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Peer Reviewed / Open Access
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[Journal Article] The Core Fucose on an IgG Antibody is an Endogenous Ligand of Dectin‐12019
Author(s)
Manabe Y., Marchetti R., Takakura Y., Nagasaki M., Nihei W., Takebe T., Tanaka K., Kabayama K., Chiodo F., Hanashima S., Kamada Y., Miyoshi E., Dulal HP., Yamaguchi Y., Adachi Y., Ohno N., Tanaka H., Silipo A., Fukase K., Molinaro A.
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Journal Title
Angewandte Chemie International Edition
Volume: 58
Issue: 51
Pages: 18697-18702
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Synthesis And Immunological Evaluation of Self-adjuvanting Anticancer Vaccine Candidate2019
Author(s)
Manabe Y., Chang TC., Feng Q., Fujimoto Y., Ohshima S., Kametani Y., Kabayama K., Nimura Y., Lin, CC., Fukase K.
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Journal Title
Peptide Science 2018
Volume: x
Related Report
Peer Reviewed
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[Presentation] Immunological Evaluation of Conjugate/Co-Assembly of Peptide Antigen and Adjuvant as Self-adjuvanting Anti-breast Cancer Candidates2020
Author(s)
Keita Ito, Yoshiyuki Manabe, Taku Aiga, Tsung-Che Chang, Kazuya Kabayama, Shino Ohshima, Yoshie Kametani, Hiroto Furukawa, Hiroshi Inaba, Kazunori Matsuura, and Koichi Fukase
Organizer
第57回ペプチド討論会
Related Report
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[Presentation] Development of Alpha-Gal Conjugated Anti-Cancer Antibodies2019
Author(s)
Fukase K., Sianturi J., Manabe Y., Li HS., Chiu LT., Chang TC., Tokunaga K., Kabayama K., Tanemura M., Takamatsu S., Miyoshi E., Hung SC.
Organizer
Eurocarb 2019
Related Report
Int'l Joint Research
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[Presentation] Activities of the Consortium for Medicine, Chemistry, and Physics at Osaka University2019
Author(s)
Kabayama K., Kaneda K., Shinohara A., Shirakami Y., Watabe T., Zhang ZJ., Toyoshima A., Yoshimura T., Manabe Y., Shimoyama A., Fukase K.
Organizer
TAT-11
Related Report
Int'l Joint Research
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