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Acquistion of unavailable soil phosphate by arbuscular mycorrhizal fungi

Research Project

Project/Area Number 18K05368
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionYamagata University

Principal Investigator

Tawaraya Keitaro  山形大学, 農学部, 教授 (70179919)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアーバスキュラー菌根菌 / 外生菌糸 / 酸性ホスファターゼ / 有機態リン酸 / 有機態リン / 菌糸の浸出物 / 菌根菌 / 不可給態リン酸 / 有機物 / フィチン酸
Outline of Final Research Achievements

Welsh onions (Allium fistulosum) were grown in two compartment pots, inoculated or uninoculated with arbuscular mycorrhizal fungi Rhizophagus clarus. The acid phosphatase activity of the soil solution was measured. The soil attached to the extraradical hyphae was collected and fractionated into Resin-P, NaHCO3-Pi, NaHCO3-Po, NaOH-Pi, NaOH-Po and HCl-P and the concentration of each phosphorus fraction was measured. The acid phosphatase activity in the soil of the hyphal fraction was higher in the inoculated than in the non-inoculated zone. Phosphorus concentrations in the fractions NaHCO3-Po and NaOH-Po tended to be lower in the inoculated than in the uninoculated plots.

Academic Significance and Societal Importance of the Research Achievements

菌糸圏の土壌溶液を採取することにより、アーバスキュラー菌根菌の外生菌糸は酸性ホスファターゼを土壌に分泌し、分泌された酸性ホスファターゼにより有機態リン酸が分解されることが示された。酸性ホスファターゼの分泌は低リンストレス下で促進された。リン資源の枯渇へ対応するため、酸性ホスファターゼ分泌能が高いアーバスキュラー菌根菌の作物への接種を行い不可給態リン酸を利用促進が有効な技術となる可能性が示された。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2020 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Secretion of acid phosphatase from extraradical hyphae of the arbuscular mycorrhizal fungus Rhizophagus clarus is regulated in response to phosphate availability2019

    • Author(s)
      Sato, T., Hachiya, S., Inamura, N., Ezawa, T., Cheng, W., and Tawaraya, K.
    • Journal Title

      Mycorrhiza

      Volume: 29 Issue: 6 Pages: 599-605

    • DOI

      10.1007/s00572-019-00923-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] アーバスキュラー菌根菌の外生菌糸から浸出される酸性ホスファターゼ活性と土壌中のリン画分の関係2020

    • Author(s)
      鈴木耶々 佐藤 匠 江沢辰広 杉原 創 程 為国 俵谷圭太郎
    • Organizer
      日本土壌肥料学会岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Metabolite profiling of hyphal exudates of arbuscular mycorrhizal fungi Rhizophagus clarus and Rhizophagus irregularis under phosphorus deficiency2020

    • Author(s)
      uri Luthfiana, Nozomi Inamura, Tantriani, Takumi Sato, Kazuki Saito Akira Oikawa Weiguo Chen and Keitaro Tawaraya
    • Organizer
      日本土壌肥料学会岡山大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] アーバスキュラー菌根菌の外生菌糸から浸出される酸性ホスファターゼ活性と土壌中のリンの形態2019

    • Author(s)
      鈴木耶々・佐藤 匠・江沢辰広・杉原 創・程 為国・俵谷圭太郎
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Arbuscular mycorrhizal fungi secrete acid phosphatase to hyphosphere in response to phosphorus deficiency2019

    • Author(s)
      Takumi Sato, Shihomi Hachiya, Nozomi Inamura, Yaya Suzuki, Tatsuhiro Ezawa, Weiguo Cheng, Keitaro Tawaraya
    • Organizer
      Rhizosphere 5
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] アーバスキュラー菌根菌が浸出する酸性ホスファターゼ活性の菌種間差2018

    • Author(s)
      佐藤匠、江沢辰広、程為国、俵谷圭太郎
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Research-status Report
  • [Presentation] アーバスキュラー菌根菌による土壌中の有機態リン酸の利用2018

    • Author(s)
      俵谷圭太郎、佐藤匠
    • Organizer
      日本土壌肥料学会2018年度神奈川大会
    • Related Report
      2018 Research-status Report
    • Invited

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Published: 2018-04-23   Modified: 2022-01-27  

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