Characteristics and community dynamics of microaerophilic iron-oxidizing bacteria in paddy field soil

• Project/Area Number: 18K05372
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 38010:Plant nutrition and soil science-related
• Research Institution: Nagoya University
• Principal Investigator: Watanabe Takeshi 名古屋大学, 生命農学研究科, 講師 (60547016)
• Project Period (FY): 2018-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 水田 / 鉄 / 鉄酸化細菌 / 鉄酸化 / 水田土壌 / 群集構造解析 / 鉄水酸化物

Outline of Final Research Achievements

The characteristics of Fe oxides formed during the growth of Gallionella-related Fe-oxidizing bacteria and dynamics of Gallionella-related Fe-oxidizing bacterial community in paddy field soils were studied to understand the role and significance of them in the Fe oxidizing process in paddy field soil. The microscopic observations and XAFS analysis showed that Gallionella-related Fe-oxidizing bacteria produced ferrihydrite-predominated iron oxides during the growth, some of which produce dendrite structures of Fe oxides. The community analysis showed that the dynamics of Gallionella-related Fe-oxidizing bacterial community changed depending on the redox cycle of Fe in paddy soils. These results suggested that Gallionella-related Fe-oxidizing bacteria are involved in the Fe-oxidizing process in paddy field soil, and their behavior may influence the dynamics of not only Fe but also other elements in the soil.

Academic Significance and Societal Importance of the Research Achievements

鉄の酸化還元反応は、水田土壌の物質代謝において重要な役割を担う。このうち、鉄の酸化反応については、これまで非生物的反応(いわゆる空気酸化)が主体と考えられ、鉄酸化細菌による生物的反応については知見が限られていた。本研究では、Gallionellaceae科の微好気性鉄酸化細菌に注目し、土壌中での鉄酸化過程に微好気性鉄酸化細菌が関与していることを初めて示した。また、非生物的な反応による鉄酸化物とは異なる特徴的な形態の鉄酸化物が一部形成されることを示した。これらの成果は、水田土壌の物質代謝のさらなる理解につながると期待され、環境や食の安全に配慮した持続可能な水稲栽培管理の確立に役立つと考えられる。

Research Products

• [Journal Article] Complete Genome Sequence of Ferrigenium kumadai An22, a Microaerophilic Iron-Oxidizing Bacterium Isolated from a Paddy Field Soil (2021)
  • Author(s): Watanabe Takeshi、Khalifa Ashraf、Asakawa Susumu
  • Journal Title: Microbiology Resource Announcements Volume: 10 Issue: 27
  • DOI: 10.1128/mra.00346-21
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Influence of alternate wetting and drying water-saving irrigation practice on the dynamics of Gallionella-related iron-oxidizing bacterial community in paddy field soil (2021)
  • Author(s): Watanabe Takeshi、Katayanagi Nobuko、Agbisit Ruth、Llorca Lizzida、Hosen Yasukazu、Asakawa Susumu
  • Journal Title: Soil Biology and Biochemistry Volume: 152 Pages: 108064-108064
  • DOI: 10.1016/j.soilbio.2020.108064
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Involvement of microaerophilic iron-oxidizing bacteria in the iron-oxidizing process at the surface layer of flooded paddy field soil (2020)
  • Author(s): Nakagawa Koki、Murase Jun、Asakawa Susumu、Watanabe Takeshi
  • Journal Title: Journal of Soils and Sediments Volume: 20 Issue: 11 Pages: 4034-4041
  • DOI: 10.1007/s11368-020-02717-w
  • Peer Reviewed
• [Journal Article] Community structure of microaerophilic iron-oxidizing bacteria in Japanese paddy field soils (2019)
  • Author(s): Naruse Tatsushi、Ban Yoshinori、Yoshida Tomofumi、Kato Takahiro、Namikawa Mari、Takahashi Tomoki、Nishida Mizuhiko、Asakawa Susumu、Watanabe Takeshi
  • Journal Title: Soil Science and Plant Nutrition Volume: 65 Issue: 5 Pages: 460-470
  • DOI: 10.1080/00380768.2019.1671139
  • NAID: 210000157214
  • Peer Reviewed
• [Presentation] 田畑輪換が土壌中の鉄酸化菌・還元菌の存在量に及ぼす影響 (2021)
  • Author(s): 松葉悠真・伊藤舞香・劉冬艶・海野裕晃・石川裕己・土屋一成・浪川茉莉・髙本慧・戸上和樹・高橋智紀・西田瑞彦・浅川晋・渡邉健史
  • Organizer: 日本土壌微生物学会2021年度大会
• [Presentation] 田畑輪換圃場の土壌中の微好気性鉄酸化細菌群集の動態 (2020)
  • Author(s): 松葉悠真・伊藤舞香・劉冬艶・海野裕晃・石川裕己・西田瑞彦・土屋一成・浪川茉莉・高本慧・戸上和樹・高橋智紀・浅川晋・渡邉健史
  • Organizer: 日本土壌肥料学会2020年度岡山大会
• [Presentation] 水稲根に生息する微好気性鉄酸化細菌の分離 (2019)
  • Author(s): 渡邉健史・伊藤舞香・ 浅川 晋
  • Organizer: 日本土壌微生物学会2019年度大会
• [Presentation] AWD 節水水田土壌中の微好気性鉄酸化細菌群集の動態 (2019)
  • Author(s): 渡邉健史・片柳薫子・Ruth Agbisit・Lizzida Llorca・宝川靖和・浅川 晋
  • Organizer: 日本土壌肥料学会2019年度静岡大会
• [Presentation] Involvement of microaerophilic iron-oxidizing bacteria in the iron-oxidizing process at the surface oxidized layer of flooded paddy field soil (2019)
  • Author(s): Nakagawa, K., Murase, J., Asakawa, S. Watanabe, T.
  • Organizer: 14th International Conference of the East and Southeast Asia Federation of Soil Sciecne Societies
  • Int'l Joint Research
• [Presentation] 水稲根圏の鉄の酸化還元反応に関わる微生物群集に関する研究 (1) 微好気性鉄酸化細菌群集の解析 (2019)
  • Author(s): 加藤健斗・尾賀俊哉・伴 佳典・Otoidobiga Cecile・Sawadogo Adama・Wonni Issa・Ouedraogo Leonard・Zongo Jean Didier・Dianou Dayeri・浅川 晋・渡邉健史
  • Organizer: 日本土壌肥料学会中部支部第99回例会・中部土壌肥料研究会第109回例会
• [Presentation] Microaerophilic iron-oxidizing bacterial community in the bulk soil rhizosphere of paddy field (2018)
  • Author(s): Kato, K., Naruse, T., Ban, Y., Yoshida, T., Funao, T., Kato, T., Namikawa, M., Takahashi, T., Nishida, M., Asakawa, S., Watanabe, T. and
  • Organizer: 21th World Congress of Soil Science
  • Int'l Joint Research