| Project/Area Number |
18K05403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
INAOKA TAKASHI 国立研究開発法人農業・食品産業技術総合研究機構, 食品研究部門, 上級研究員 (40391205)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | グルコース6リン酸デヒドロゲナーゼ / ペントースリン酸経路 / NADPH / オートインデューサー / ネオトレハロサジアミン / カノサミン / メタボローム / 枯草菌 / 二次代謝 / TCA回路 / NTD / 代謝 |
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| Outline of Final Research Achievements |
The pentose phosphate (PP) pathway is one of the major sources of cellular NADPH. A B. subtilis zwf mutant that lacks glucose-6-phosphate dehydrogenase (the enzyme that catalyzes the first step of the PP pathway) showed inoculum-dose-dependent growth. This growth defect was suppressed by glcP disruption, which causes the upregulation of an autoinducer neotrehalosadiamine (NTD)/kanosamine biosynthetic pathway. A metabolome analysis showed that the stimulation of NTD/kanosamine biosynthesis caused significant accumulation of TCA cycle intermediates and NADPH. Because the major malic enzyme YtsJ concomitantly generates NADPH through malate-to-pyruvate conversion, de novo NTD/kanosamine biosynthesis can result in an increase in the intracellular NADPH pool via the accumulation of malate. In fact, a zwf mutant grew in malate-supplemented medium. Our results suggest that NTD/kanosamine has the potential to modulate the carbon-energy metabolism through an autoinduction mechanism.
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| Academic Significance and Societal Importance of the Research Achievements |
枯草菌を含む複数のバチルス属細菌が生産するネオトレハロサジアミン（NTD）はカノサミン2分子が結合した機能不明のオートインデューサーである。NTD/カノサミンは自身の生合成経路を活性化することによりTCA回路の代謝中間体を増大させることが判明した。これはNTD/カノサミンが細菌の中心的な代謝経路を直接または間接的に調節することを示唆しており、菌体外物質を活用した細菌集団の代謝制御技術の開発へと繋がるものである。
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