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Analysis of mutant strains of a cyanobacteria Syenchococcus which have adapted to high temperature conditions.

Research Project

Project/Area Number 18K05416
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionShizuoka University

Principal Investigator

Kanesaki Yu  静岡大学, グリーン科学技術研究所, 特任助教 (70380293)

Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords高温ストレス / シアノバクテリア / 適応進化 / 突然変異 / ゲノム解析 / リシーケンス解析 / 次世代シーケンサー / トランスクリプトーム / 環境適応 / NGS解析 / リシーケンス / NGS
Outline of Final Research Achievements

A long-term laboratory evolution experiment was attempted to challenge the proposition that mesophylic cyanobacteria can adapt to high temperatures beyond their growth limit temperatures. Previous studies had succeeded in obtaining a line of strains that had grown well above the temperature limit of the strain. Electron microscopic analysis confirmed structural changes in the cells. We were also able to identify trade-offs in the temperature range at which the line could grow compared to the parental line. To confirm reproducibility, we have constracted two new lines, but these lines had different mutations from the existing lines.

Academic Significance and Societal Importance of the Research Achievements

ある生物が、自らの至適生育環境から生育限界を超えた環境条件に適応するのにどれだけの自然突然変異の蓄積が必要か、どれだけ時間がかかるか、という命題の実験的な証明は微生物の進化機構や環境適応速度を見積もる上で極めて重要な基礎研究である。また自然突然変異だけで作製された変異株は屋外での培養が可能なため、今後の有用物質生産株の作出に向けた指標情報としても有用である。また将来的な地球温暖化などでどのように微生物の表現型が変化していくかを予想する上でも重要な成果である。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (48 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (22 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 21 results,  Open Access: 17 results) Presentation (23 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Int'l Joint Research] University of Montana(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Montana(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] University Freiburg(ドイツ)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Reacquisition of light-harvesting genes in a marine cyanobacterium confers a broader solar niche.2021

    • Author(s)
      Ulrich NJ, Uchida H, Kanesaki Y, Hirose E, Murakami A, Miller SR.
    • Journal Title

      Current Biology

      Volume: 31 Issue: 7 Pages: 1539-1546.e4

    • DOI

      10.1016/j.cub.2021.01.047

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid.2021

    • Author(s)
      Matsuzaki R., Suzuki S., Yamaguchi H., Kawachi M., Kanesaki Y., Yoshikawa H., Mori T. and Nozaki H.
    • Journal Title

      BMC Ecology and Evolution

      Volume: 21 Issue: 1 Pages: 11-11

    • DOI

      10.1186/s12862-020-01733-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete sequence and structure of the genome of the harmful algal bloom-forming cyanobacterium Planktothrix agardhii NIES-204T and detailed analysis of secondary metabolite gene clusters.2021

    • Author(s)
      Shimura Y, Fujisawa T, Hirose Y, Misawa N, Kanesaki Y, Nakamura Y, Kawachi M.
    • Journal Title

      Harmful Algae

      Volume: 101 Pages: 101942-101942

    • DOI

      10.1016/j.hal.2020.101942

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The CRP-family transcriptional regulator DevH regulates expression of heterocyst-specific genes at the later stage of differentiation in the cyanobacterium Anabaena sp. strain PCC 71202020

    • Author(s)
      Kurio Yohei, Koike Yosuke, Kanesaki Yu, Watanabe Satoru, Ehira Shigeki
    • Journal Title

      Molecular Microbiology

      Volume: - Issue: 4 Pages: 553-562

    • DOI

      10.1111/mmi.14558

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] H-NS Family Proteins Drastically Change Their Targets in Response to the Horizontal Transfer of the Catabolic Plasmid pCAR12020

    • Author(s)
      Nakamura T, Suzuki-Minakuchi C, Kawano H, Kanesaki Y, Kawasaki S, Okada K, Nojiri H.
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 1099-1099

    • DOI

      10.3389/fmicb.2020.01099

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Changes in the transcriptome, ploidy, and optimal light intensity of a cryptomonad upon integration into a kleptoplastic dinoflagellate2020

    • Author(s)
      Onuma R, Hirooka S, Kanesaki Y, Fujiwara T, Yoshikawa H, Miyagishima SY.
    • Journal Title

      The ISME Journal

      Volume: 14 Issue: 10 Pages: 2407-2423

    • DOI

      10.1038/s41396-020-0693-4

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evolutionary Changes in DnaA-Dependent Chromosomal Replication in Cyanobacteria2020

    • Author(s)
      Ohbayashi Ryudo、Hirooka Shunsuke、Onuma Ryo、Kanesaki Yu、Hirose Yuu、Kobayashi Yusuke、Fujiwara Takayuki、Furusawa Chikara、Miyagishima Shin-ya
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 786-786

    • DOI

      10.3389/fmicb.2020.00786

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acclimation process of the chlorophyll <i>d</i>-bearing cyanobacterium <i>Acaryochloris marina</i> to an orange light environment revealed by transcriptomic analysis and electron microscopic observation2020

    • Author(s)
      Kashimoto Tomonori、Miyake Keita、Sato Mayuko、Maeda Kaisei、Matsumoto Chikahiro、Ikeuchi Masahiko、Toyooka Kiminori、Watanabe Satoru、Kanesaki Yu、Narikawa Rei
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 66 Issue: 2 Pages: 106-115

    • DOI

      10.2323/jgam.2019.11.008

    • NAID

      130007855496

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] RNase E-dependent degradation of tnaA mRNA encoding tryptophanase is prerequisite for the induction of acid resistance in Escherichia coli.2020

    • Author(s)
      Kanda T, Abiko G, Kanesaki Y, Yoshikawa H, Iwai N, Wachi M.
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 7128-7128

    • DOI

      10.1038/s41598-020-63981-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 希少生物スイゼンジノリの自生地の現状と保全に向けた取組み2020

    • Author(s)
      谷口智之, 兼崎友, 金子慎一郎, 大城香
    • Journal Title

      バイオサイエンスとインダストリー(B&I)

      Volume: 78 Pages: 350-353

    • Related Report
      2020 Research-status Report
  • [Journal Article] Genes Regulated by Branched-Chain Polyamine in the Hyperthermophilic Archaeon Thermococcus Kodakarensis.2020

    • Author(s)
      Fukuda W, Yamori Y, Hamakawa M, Osaki M, Fukuda M, Hidese R, Kanesaki Y, Okamoto-Kainuma A, Kato S, Fujiwara S.
    • Journal Title

      Amino Acids

      Volume: 52 Issue: 2 Pages: 287-299

    • DOI

      10.1007/s00726-019-02793-4

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Coordination of Polyploid Chromosome Replication With Cell Size and Growth in a Cyanobacterium.2019

    • Author(s)
      Ohbayashi R, Nakamachi A, Hatakeyama TS, Watanabe S, Kanesaki Y, Chibazakura T, Yoshikawa H, Miyagishima SY.
    • Journal Title

      mBio

      Volume: 10 Issue: 2

    • DOI

      10.1128/mbio.00510-19

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete Genome Sequence of Enterococcus Faecium QU50, a Thermophilic Lactic Acid Bacterium Capable of Metabolizing Xylose.2019

    • Author(s)
      Abe K, Kanesaki Y, Abdel-Rahman MA, Watanabe S, Zendo T, Chibazakura T, Shimizu-Kadota M, Sonomoto K, Yoshikawa H.
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 21

    • DOI

      10.1128/mra.00413-19

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Capacity for Survival in Global Warming: Adaptation of Mesophiles to the Temperature Upper Limit.2019

    • Author(s)
      Kosaka T, Nakajima Y, Ishii A, Yamashita M, Yoshida S, Murata M, Kato K, Shiromaru Y, Kato S, Kanesaki Y, Yoshikawa H, Matsutani M, Thanonkeo P, Yamada M.
    • Journal Title

      PLoS One

      Volume: 14 Issue: 5 Pages: e0215614-e0215614

    • DOI

      10.1371/journal.pone.0215614

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interplay and Targetome of the Two Conserved Cyanobacterial sRNAs Yfr1 and Yfr2 in Prochlorococcus MED4.2019

    • Author(s)
      Lambrecht SJ, Kanesaki Y, Fuss J, Huettel B, Reinhardt R, Steglich C.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 14331-14331

    • DOI

      10.1038/s41598-019-49881-9

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physiological properties and genetic analysis related to exopolysaccharide (EPS) production in the fresh-water unicellular cyanobacterium <i>Aphanothece sacrum</i> (Suizenji Nori)2019

    • Author(s)
      Ohki K, Kanesaki Y, Suzuki N, Okajima M, Kaneko T, Yoshikawa S.
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 65 Issue: 1 Pages: 39-46

    • DOI

      10.2323/jgam.2018.04.004

    • NAID

      130007608428

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative genome analysis of Aspergillus flavus clinically isolated in Japan.2019

    • Author(s)
      Toyotome T, Hamada S, Yamaguchi S, Takahashi H, Kondoh D, Takino M, Kanesaki Y, Kamei K.
    • Journal Title

      DNA Research

      Volume: 26 Issue: 1 Pages: 95-103

    • DOI

      10.1093/dnares/dsy041

    • NAID

      120006603073

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete Genome Sequence of the Marine Bacterium Erythrobacter flavus Strain KJ5.2019

    • Author(s)
      Kanesaki Y, Setiyono E, Pringgenies D, Moriuchi R, Brotosudarmo THP, Awai K.
    • Journal Title

      Microbiology Resource Announcements

      Volume: 8 Issue: 13

    • DOI

      10.1128/mra.00140-19

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional activation of glycogen catabolism and oxidative pentose phosphate pathway by NrrA facilitates cell survival under nitrogen starvation in the cyanobacterium Synechococcus sp. strain PCC 7002.2018

    • Author(s)
      Shimmori Y, Kanesaki Y, Nozawa M, Yoshikawa H, and Ehira S.
    • Journal Title

      Plant and Cell Physiology

      Volume: 印刷中 Issue: 6 Pages: 1225-1233

    • DOI

      10.1093/pcp/pcy059

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intracellular free flavin and its associated enzymes participate in oxygen and iron metabolism in Amphibacillus xylanus lacking a respiratory chain.2018

    • Author(s)
      Kimata S, Mochizuki D, Satoh J, Kitano K, Kanesaki Y, Takeda K, Abe A, Kawasaki S, Niimura Y.
    • Journal Title

      FEBS Open Bio

      Volume: 9 Issue: 6 Pages: 947-961

    • DOI

      10.1002/2211-5463.12425

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Linear Regression Links Transcriptomic Data and Cellular Raman Spectra2018

    • Author(s)
      Kobayashi-Kirschvink, K. J., Nakaoka, H., Oda, A., Kamei, K. F., Nosho, K., Fukushima, H., Kanesaki, Y., Yajima, S., Masaki, H., Ohta, K., Wakamoto, Y.
    • Journal Title

      Cell Systems

      Volume: 7 Issue: 1 Pages: 104-117

    • DOI

      10.1016/j.cels.2018.05.015

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Newly Identified Nucleoid-Associated-Like Protein YlxR Regulates Metabolic Gene Expression in Bacillus subtilis2018

    • Author(s)
      Ogura M and Kanesaki Y
    • Journal Title

      mSphere

      Volume: 3 Issue: 5

    • DOI

      10.1128/msphere.00501-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 糸状性ラン藻 Pseudanabaena株シグマ因子SigBの特徴づけと発現解析2021

    • Author(s)
      渡邉 明花、菅原 卓也、鎭西 真理子、兼崎 友、中平 洋一、朝山 宗彦
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 藍藻ゲノムの可塑性から進化について考える2021

    • Author(s)
      兼崎 友
    • Organizer
      第21回 静岡ライフサイエンスシンポジウム 「生物学×情報学~ コンピューターが加速させるバイオ研究の最前線 ~」
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 食用藍藻スピルリナが生産する細胞外高分子物質に関する研究2021

    • Author(s)
      坂巻 裕, 前田 海成, 兼崎 友, 大森 正之, 朝井 計, 渡辺 智
    • Organizer
      日本農芸化学会 2021年大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 糸状性ラン藻 Pseudanabaena株シグマ因子SigBの特徴づけと発現解析2020

    • Author(s)
      渡邉 明花,菅原 卓也,鎭西 真理子,兼崎 友,中平 洋一,朝山 宗彦
    • Organizer
      第43回 日本分子生物学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 長期高温培養による適応進化実験からシアノバクテリアの可能性を探る2020

    • Author(s)
      兼崎 友, 渡辺 智
    • Organizer
      日本農芸化学会 2020年大会
    • Related Report
      2019 Research-status Report
  • [Presentation] シアノバクテリアの染色体複製におけるDnaA依存性の進化2019

    • Author(s)
      大林 龍胆, 廣岡 俊亮, 大沼 亮, 広瀬 侑, 兼崎 友, 宮城島 進也
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] シアノバクテリアにおける非典型的なDNA複製機構2019

    • Author(s)
      渡辺 智, 山崎 脩平, 青柳 智大, 松根(荷村) かおり, 兼崎 友, 大林 龍胆
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] シアノバクテリアSynechocystis sp. PCC 6803における染色体とプラスミドの維持機構の解析2019

    • Author(s)
      青柳智大, 山崎脩平, 大林龍胆, 兼崎 友, 荷村(松根)かおり, 渡辺 智
    • Organizer
      藍藻の分子生物学2019
    • Related Report
      2019 Research-status Report
  • [Presentation] 遺伝子組み換えにより従属栄養増殖を可能にした単細胞紅藻シゾンの解析2019

    • Author(s)
      向井 瑞梨, 兼崎 友, 宮澤 和己, 島田 尚弥, 池内 昌彦, 藤原 崇之, 宮城島 進也, 渡辺 智
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 紅藻ガルデリアの新規単離株と変異株の従属栄養応答の解析2019

    • Author(s)
      兼崎 友, 宮澤 和己, 向井 瑞梨, 重信 直人, 渡辺 智
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] シアノバクテリアAcaryochloris marinaの長期橙色光培養による実験室内進化2019

    • Author(s)
      成川 礼, 松本 直大, 樫本 友則, 佐藤 繭子, 渡辺 智, 豊岡 公徳, 池内 昌彦, 兼崎 友
    • Organizer
      第13回日本ゲノム微生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] DnaA-oriCに依存しないシアノバクテリアSynechocystis sp. PCC 6803の複製開始機構2019

    • Author(s)
      山崎 脩平, 青栁 智大, 荷村 かおり, 兼崎 友, 大林 龍胆, 渡辺 智
    • Organizer
      第13回日本ゲノム微生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] プラスミドインテグレーションにより駆動するシアノバクテリアDNA複製開始機構2019

    • Author(s)
      青柳 智大, 山崎 脩平, 兼崎 友, 荷村 かおり, 大林 龍胆, 渡辺 智
    • Organizer
      第13回日本ゲノム微生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Physiological properties and genetic analysis related to exopolysaccharide (EPS) production in the cyanobacterium Chroococcus sp. FPU101.2018

    • Author(s)
      Kaori Ohki, Yu Kanesaki, Uemura Akira, Maiko Okajima, Tatsuo Kaneko, Shinya Yoshikawa
    • Organizer
      The International Symposium on Phototrophic Prokaryotes 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] DevH is a global regulator of the heterocyst-specific genes that are upregulated at the later stage of differentiation.2018

    • Author(s)
      Youhei Kurio, Yousuke Koike, Yu Kanesaki, Hirofumi Yoshikawa, Shigeki Ehira
    • Organizer
      The International Symposium on Phototrophic Prokaryotes 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Activation of glycogen catabolism by NrrA facilitates cell survival under nitrogen starvation in the cyanobacterium Synechococcus sp. PCC 7002.2018

    • Author(s)
      Yuka Shimmori, Yu Kanesaki, Masafumi Nozawa, Hirofumi Yoshikawa, Shigeki Ehira
    • Organizer
      The International Symposium on Phototrophic Prokaryotes 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 長期高温培養によるシアノバクテリアの適応進化実験と突然変異の解析2018

    • Author(s)
      兼崎 友
    • Organizer
      国立遺伝学研究所公募型共同研究・研究会 NIG-JOINT:微生物生態から見えてくる新しい生理機能とその応用
    • Related Report
      2018 Research-status Report
  • [Presentation] DnaA-oriC に依存しないシアノバ クテリアのDNA 複製機構2018

    • Author(s)
      渡辺智、山﨑脩平、青柳智大、松根(荷村)かおり、兼崎友、大林龍胆
    • Organizer
      第90回 日本遺伝学会
    • Related Report
      2018 Research-status Report
  • [Presentation] シアノバクテリアSynechocystis sp. PCC 6803 における複製開始機構の解析2018

    • Author(s)
      山﨑脩平、大林龍胆、松根(荷村)かおり、兼崎友、渡辺智
    • Organizer
      第90回 日本遺伝学会
    • Related Report
      2018 Research-status Report
  • [Presentation] シアノバクテリアSynechocystis sp. PCC 6803 におけるRecA タンパク質の機能解析2018

    • Author(s)
      青柳 智大、栗原 亜実、大林 龍胆、兼崎 友、渡辺 智
    • Organizer
      第90回 日本遺伝学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 新奇糸状性シアノバクテリアのSigB相同性因子の発現と機能解析2018

    • Author(s)
      菅原 卓也, 鎭西 真理子, 中平 洋一, 兼崎 友, 吉川 博文, 朝山 宗彦
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 陸棲藍藻の宇宙環境に関わる遺伝子の検索2018

    • Author(s)
      加藤 浩, 広瀬 侑, 兼崎 友, 藤澤 貴智, 中村 保一, 吉川 博文, 木村 駿太, 富田-横谷 香織
    • Organizer
      日本地球惑星科学連合2018年大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Genes of terrestrial cyanobacteria which are expected to be useful in space environments.2018

    • Author(s)
      Katoh Hiroshi, Tomita-Yokotani Kaori, Kimura Shunta, Hirose, Yuu, Kanesaki Yu, Fujisawa Takatomo, Nakamura Yasukazu, Yoshikawa Hirofumi.
    • Organizer
      42nd COSPAR Scientific Assembly
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2023-01-30  

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