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Microbial-mediated production of lipids capable control of skin microbiome

Research Project

Project/Area Number 18K05426
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionOsaka Research Institute of Industrial Science and Technology

Principal Investigator

Nagao Toshihiro  地方独立行政法人大阪産業技術研究所, 森之宮センター, 研究部長 (30416309)

Project Period (FY) 2018-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords皮膚細菌叢 / Staphylococcus aureus / S. epidermidis / 抗菌活性 / パルミトレイン酸 / リン脂質 / 皮膚菌叢
Outline of Final Research Achievements

Palmitoleic acid (POA) shows selective antibacterial activity which suppress the growth of harmful microorganism Staphylococcus aureus and does not suppress the growth of beneficial microorganism Staphylococcus epidermidis. In this study, we produced POA-containing phospholipids through phospholipase reaction with lyso-phospholipids because only S. aureus possesses enzymes hydrolyzing non-active POA-containing phospholipids to active POA. However the synthesized POA-containing phospholipids did not shows meaningful selective antibacterial activity.

Academic Significance and Societal Importance of the Research Achievements

近年、大腸、皮膚、口腔の常在微生物と健康・疾病の関係の研究が進展し、有用微生物を用いた臨床試験研究も進展し、腸内細菌に関する商品が出始めている。皮膚細菌叢については、アメリカにおいて、黄色ブドウ球菌だけの生育を抑制する微生物を用いたアトピー患者に対する臨床試験研究で効果が得られており、黄色ブドウ球菌の生育を抑制し、表皮ブドウ球菌の生育を抑制しないという選択的抗菌活性は有用であることが分かった。POAが持つ選択的抗菌活性の場合、エステル型ではなく、遊離型にしなければ効果を発揮できない事が本研究で判明し、今後、遊離型のPOAの活用が期待できる。

Report

(6 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (30 results)

All 2023 2022 2021 2020 2019 2018

All Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 7 results) Presentation (17 results) (of which Int'l Joint Research: 5 results,  Invited: 8 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Production of a selective antibacterial fatty acid against Staphylococcus aureus by Bifidobacterium strains2023

    • Author(s)
      Hiroshi Kikukawa, Toshihiro Nagao, Mitsuki Ota, Shigeo Takashima, Kohji Kitaguchi, Emiko Yanase, Sadatoshi Maeda, Kiyotaka Y. Hara
    • Journal Title

      Microbiome Res. Rep.

      Volume: 2 Issue: 1 Pages: 4-4

    • DOI

      10.20517/mrr.2022.24

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Antimicrobial Activities of LL37 Fragment Mutant-Poly (Lactic-Co-Glycolic) Acid Conjugate Against Staphylococcus aureus, Escherichia coli, and Candida albicans2021

    • Author(s)
      Takeshi Mori, Miyako Yoshida, Mai Hazekawa, Daisuke Ishibashi, Yoshiro Hatanaka, Toshihiro Nagao, Rie Kakehashi, Honami Kojima, Rio Uno, Minoru Ozeki, Ikuo Kawasaki, Taku Yamashita, Junichi Nishikawa, and Takahiro Uchida
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 22 Issue: 10 Pages: 5097-5107

    • DOI

      10.3390/ijms22105097

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Targeted Delivery of Miconazole Employing LL37 Fragment Mutant Peptide CKR12-Poly (Lactic-Co-Glycolic) Acid Polymeric Micelles2021

    • Author(s)
      Takeshi Mori, Miyako Yoshida, Mai Hazekawa, Daisuke Ishibashi, Yoshiro Hatanaka, Rie Kakehashi, Makoto Nakagawa, Toshihiro Nagao, Miki Yoshii, Honami Kojima, Rio Uno, and Takahiro Uchida
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 22 Issue: 21 Pages: 12056-12069

    • DOI

      10.3390/ijms222112056

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 皮膚細菌叢を制御する脂肪酸2020

    • Author(s)
      永尾寿浩、宇山彩香、田中重光、杉野哲造
    • Journal Title

      生物工学会誌

      Volume: 98 Pages: 525-528

    • Related Report
      2020 Research-status Report
    • Open Access
  • [Journal Article] Health Beneficial Food Emulsifier Produced from Fishery Byproducts2020

    • Author(s)
      Toshihiro Nagao, Shu Takahashi, Hideyuki Kurihara, Koretaro Takahashi
    • Journal Title

      Journal of Oleo Science

      Volume: 69 Issue: 10 Pages: 1231-1240

    • DOI

      10.5650/jos.ess20145

    • NAID

      130007923322

    • ISSN
      1345-8957, 1347-3352
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Control of skinmicrobiota with fatty acids: rethinking the removal of “all” skin microorganisms.2020

    • Author(s)
      T. Nagao, A. Uyama, T. Sugino, S. Tanaka, N. Kishimoto
    • Journal Title

      INFORM

      Volume: 31 Issue: 1 Pages: 12-16

    • DOI

      10.21748/inform.01.2020.12

    • Related Report
      2019 Research-status Report
  • [Journal Article] Effect of Dietary Oil Rich in Docosahexaenoic Acid-Bound Lysophosphatidylcholine Prepared from Fishery By-Products on Lipid and Fatty Acid Composition in Rat Liver and Brain2019

    • Author(s)
      Ryota Hosomi, Kenji Fukunaga, Toshihiro Nagao, Shunichi Shiba, Kazumasa Miyauchi, Munehiro Yoshida, and Koretaro Takahash
    • Journal Title

      Journal of Oleo Science

      Volume: 68 Issue: 8 Pages: 781-792

    • DOI

      10.5650/jos.ess19103

    • NAID

      130007686325

    • ISSN
      1345-8957, 1347-3352
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of Dietary Partial Hydrolysate of Phospholipids, Rich in Docosahexaenoic Acid-Bound Lysophospholipids, on Lipid and Fatty Acid Composition in Rat Serum and Liver.2019

    • Author(s)
      Ryota Hosomi , Kenji Fukunaga, Toshihiro Nagao, Toshifumi Tanizaki, Kazumasa Miyauchi, Munehiro Yoshida, Seiji Kanda, Toshimasa Nishiyama, and Koretaro Takahashi
    • Journal Title

      Journal of Food Science

      Volume: 84 Issue: 1 Pages: 183-191

    • DOI

      10.1111/1750-3841.14416

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 大阪発フードイノベーション, 大阪産業技術研究所における食品産業支援2019

    • Author(s)
      畠中芳郎, 村上洋, 永尾寿浩
    • Journal Title

      FOOD STYLE21

      Volume: 25 Pages: 17-20

    • Related Report
      2018 Research-status Report
  • [Journal Article] アクネ菌株選択的抗菌剤2018

    • Author(s)
      杉野哲造, 宇山彩香, 鈴木健吾, 永尾寿浩
    • Journal Title

      フレグランスジャーナル

      Volume: 46 Pages: 46-50

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Journal Article] Screening of fatty acids showing selective antibacterial activity against acne-associated Propionibacterium acnes.2018

    • Author(s)
      T. Nagao
    • Journal Title

      INFORM

      Volume: 29 Pages: 13-14

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Presentation] 皮膚細菌叢を制御する脂質2023

    • Author(s)
      永尾寿浩
    • Organizer
      化粧品開発展_東京
    • Related Report
      2022 Annual Research Report
  • [Presentation] 黄色ブドウ球菌と表皮ブドウ球菌に対する選択的抗菌活性に及ぼす脂肪酸の炭素数と二重結合数の影響2022

    • Author(s)
      永尾寿浩、吉井未貴、田中重光、菊川 史、鈴木徹
    • Organizer
      日本生物工学会創立100周年記念大会 (第74回日本生物工学会大会)
    • Related Report
      2022 Annual Research Report
  • [Presentation] 皮膚細菌叢を制御する脂肪酸とそれを活用した企業への製品化支援2021

    • Author(s)
      永尾寿浩
    • Organizer
      Web油脂実践講座2021
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 皮膚細菌叢を制御する脂質およびそれを活用した企業への製品化支援2021

    • Author(s)
      永尾寿浩
    • Organizer
      産総研・産技連 LS-BT合同研究発表会、産技連 ライフサイエンス部会 バイオテクノロジー分科会/ 研究成果・実用化事例発表会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 皮膚細菌叢を制御する脂質2020

    • Author(s)
      永尾寿浩
    • Organizer
      石けん洗剤技術交流会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 皮膚細菌叢を制御する脂質:公設試における基礎研究と企業支援2020

    • Author(s)
      永尾寿浩
    • Organizer
      日本生物工学会Webシンポジウム2020
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] スキンフローラをコントロールする技術とその応用、疾病に関与する微生物を選択的に抑制する脂肪酸2020

    • Author(s)
      永尾寿浩
    • Organizer
      第181回 彩都バイオサイエンスセミナー
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 皮膚細菌叢を制御する脂質2020

    • Author(s)
      永尾寿浩
    • Organizer
      日本生物工学会 脂質駆動学術産業創生研究部会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Production of palmitoleic acid-rich triacylglycerols by Saccharomyces cerevisiae to control of skin microbiome.2019

    • Author(s)
      Toshihiro Nagao, Kazue Narihiro, Shigemitsu Tanaka, Kazuyoshi Kimura, Kazuhiro Yamashita, Yasushi Kamisaka
    • Organizer
      AOCS Annual Meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Control of skin microbiome with fatty acids.2019

    • Author(s)
      Toshihiro Nagao, Ayaka Uyama, Shigemitsu Tanaka, Teizo Sugino
    • Organizer
      ISBAB 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Control of skin microbiota with fatty acids2019

    • Author(s)
      Toshihiro Nagao, Ayaka Uyama, Shigemitsu Tanaka, Teizo Sugino
    • Organizer
      ICoFF2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 皮膚細菌叢を制御する脂質2019

    • Author(s)
      永尾寿浩
    • Organizer
      油化学会東海支部講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 脂質の選択的抗菌活性による皮膚細菌叢の健全化2019

    • Author(s)
      永尾寿浩
    • Organizer
      関西バイオビジネスマッチング2019
    • Related Report
      2018 Research-status Report
  • [Presentation] 皮膚菌叢をコントロールする パルミトレイン酸を高濃度含有する油脂の Saccharomyces cerevisiaeによる生産2019

    • Author(s)
      永尾寿浩、成廣和、田中重光、木村和義、山下和彦、神坂泰
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Screening of fatty acids showing selective antibacterial activity against acne-associated Propionibacterium acnes.2018

    • Author(s)
      Ayaka Uyama, Teizo Sugino, Shigemitsu Tanaka, Toshihiro Nagao
    • Organizer
      2018 AOCS Annual Meeting & Expo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酵素法による機能性脂質の製造と精製および皮膚の微生物を健全化する脂質2018

    • Author(s)
      永尾寿浩
    • Organizer
      2018年若手の会サマースクール
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Control of skin microbiome with fatty acids.2018

    • Author(s)
      Toshihiro Nagao
    • Organizer
      ISBAB 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Book] 天然系抗菌・防カビ剤の開発と応用2019

    • Author(s)
      永尾寿浩
    • Publisher
      CMC出版
    • ISBN
      9784781312606
    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] 脂質および脂肪酸組成物の製造方法ならびに脂肪酸組成物2018

    • Inventor(s)
      永尾寿浩,田中重光,山下和彦,成廣和枝,木村和義,神坂泰
    • Industrial Property Rights Holder
      大阪産業技術研究所、ヤヱガキ醗酵技研、産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-132703
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2024-01-30  

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