| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we investigated the mechanism of amino acid transport by yeast vacuolar PQ-loop proteins and indicated that the exchange activity of arginine/histidine across the vacuolar membrane of Saccharomyces cerevisiae is dependent on Ypq2. Furthermore, we identified Stm1 of Schizosaccharomyces pombe as a novel vacuolar membrane protein involved in the transport of basic amino acids. From the results of amino acid determination of vacuolar fractions of the mutants disrupted genes encoding these PQ-loop proteins, it was suggested that these vacuolar PQ-loop proteins operate at the vacuolar membrane for the export of basic amino acids, especially arginine, from vacuoles.
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