| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Nuclear-encoded chloroplastic proteins are imported into chloroplasts by utilizing the unique translocation machinery (translocon) embedded in the double-envelope membranes from the cytosol after translated as precursors. Most of knowledge regarding protein import have been gained through the analyses of the early-protein translocation intermediates (PTIs) formed under limited energy conditions, in which precursors are trapped in the translocon. However, many questions regarding molecular actions during translocation after release of precursors from the early PTIs have remained unsolved. During the research period, I have designed and prepared precursors carrying steric hindrance which may plug translocon channel to form PTIs under translocation conditions to analyze the protein-protein interactions between precursors and the translocon component(s) and intra-interactions within the translocon.
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