| Project/Area Number |
18K05445
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38030:Applied biochemistry-related
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
Tanaka Shun-ichi 京都府立大学, 生命環境科学研究科, 准教授 (70591387)
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| Co-Investigator(Kenkyū-buntansha) |
松村 浩由 立命館大学, 生命科学部, 教授 (30324809)
吉澤 拓也 立命館大学, 生命科学部, 助教 (50779056)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | タンパク質工学 / 人工結合タンパク質 / 酵素 / 基質特異性 / 進化分子工学 |
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| Outline of Final Research Achievements |
In this study, to explore the application possibility of our novel enzyme-engineering strategy, Enzyme Engineering by Proxy, we attempted to elucidate the molecular mechanistic aspect of the strategy and conducted a comprehensive analysis on various enzyme systems. We determined the crystal structure of a specificity-modifying proxy protein in complex with a β-galactosidase. This structure and mutational analysis identified key elements important for altering enzyme’s specificity. In addition, we successfully altered substrate specificity of a lipase, suggesting that our strategy can be applied to diverse enzyme systems.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、酵素工学における新しい方法論の創出を志向するものであり、学術的意義は大きいと考えられる。また、本技術の更なる発展は、従来の手法では難しく諦められていた酵素の機能改変が実現され、新しい食品素材、医薬品、工業原料などの創出に繋がることが期待できる。したがって、本研究成果は学術・産業の両面において意義は大きいと考えられる。
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