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Bioorganic chemistry research on novel germination stimulants derived from carlactone

Research Project

Project/Area Number 18K05452
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38040:Bioorganic chemistry-related
Research InstitutionUtsunomiya University

Principal Investigator

XIE Xiaonan  宇都宮大学, バイオサイエンス教育研究センター, 准教授 (30610323)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsストリゴラクトン / 植物ホルモン / 非典型ストリゴラクトン / イオンモビリティ / 質量分析 / 非典型SL / 非典型的SL
Outline of Final Research Achievements

In the rhizosphere, strigolactones (SLs) induce seed germination in root parasitic weeds and promote root colonization by arbuscular mycorrhizal fungi. In plants, SLs function as a novel class of hormones regulating plant architecture. In this study, structural diversity, distribution in the plant kingdom, and biological functions of SLs are summarized. As a result, five novel noncanonical SLs were isolated and structurally determined from legumes, grasses, asteraceae and bryophytes. Furthermore, we have developed an ultrasensitive analytical method for SL using an ion mobility mass spectrometer, and succeeded in detecting SL in microtissues of 1 mg.

Academic Significance and Societal Importance of the Research Achievements

植物生産するストリゴラクトンはAM 菌の共生に必須なシグナル分子であり、さらには植物地上部および地下部の形態形成を制御する植物ホルモンである。本研究の研究成果はSL生産・分泌に及ぼす植物栄養素に影響の解析、根寄生植物の種子発芽におけるSLの構造活性相関の解析などにも関わり、SLおよび根寄生植物に関する基礎的研究を幅広く推進することができる。また、枝分かれの制御は植物の形態形成や農業生産に直接影響していることから、本研究は国連の持続可能な開発目標(SDGs)の目標2「飢餓をゼロに」と15「陸上の豊かさを守る」などにも貢献できる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (34 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (3 results) Journal Article (16 results) (of which Int'l Joint Research: 16 results,  Peer Reviewed: 16 results,  Open Access: 9 results) Presentation (15 results) (of which Invited: 1 results)

  • [Int'l Joint Research] ケンブリッジ大学(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 南京農業大学(中国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] 南京農業大学(中国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] Major components of the KARRIKIN INSENSITIVE2-dependent signaling pathway are conserved in the liverwort Marchantia polymorpha2021

    • Author(s)
      Mizuno Y, Komatsu A, Shimazaki S, Naramoto S, Inoue K, Xie X, Ishizaki K, Kohchi T, Kyozuka J.
    • Journal Title

      Plant Cell

      Volume: - Issue: 7 Pages: 112-156

    • DOI

      10.1093/plcell/koab106

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SPL14/17 act downstream of strigolactone signalling to modulate rice root elongation in response to nitrate supply2021

    • Author(s)
      Sun Huwei、Guo Xiaoli、Qi Xuejiao、Feng Fan、Xie Xiaonan、Zhang Yali、Zhao Quanzhi
    • Journal Title

      The Plant Journal

      Volume: 21 Issue: 3 Pages: 11-11

    • DOI

      10.1111/tpj.15188

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] S-Adenosylhomocysteine Analogue of a Fairy Chemical, Imidazole-4-carboxamide, as its Metabolite in Rice and Yeast and Synthetic Investigations of Related Compounds2021

    • Author(s)
      Hitoshi Ouchi , Takuya Namiki , Kenji Iwamoto , Nobuo Matsuzaki , Makoto Inai , Mihaya Kotajima , Jing Wu , Jae-Hoon Choi , Yoko Kimura , Hirofumi Hirai , Xiaonan Xie , Hirokazu Kawagishi , Toshiyuki Kan
    • Journal Title

      J Nat Prod .

      Volume: 84 Issue: 2 Pages: 453-458

    • DOI

      10.1021/acs.jnatprod.0c01269

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genetic and functional diversity of PsyI/PsyR quorum-sensing system in the Pseudomonas syringae complex2020

    • Author(s)
      Morohoshi Tomohiro、Oshima Akinori、Xie Xiaonan、Someya Nobutaka
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 97 Issue: 2

    • DOI

      10.1093/femsec/fiaa254

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis2020

    • Author(s)
      Yoneyama Kaori、Akiyama Kohki、Brewer Philip B.、Mori Narumi、Kawano‐Kawada Miyuki、Haruta Shinsuke、Nishiwaki Hisashi、Yamauchi Satoshi、Xie Xiaonan、Umehara Mikihisa、Beveridge Christine A.、Yoneyama Koichi、Nomura Takahito
    • Journal Title

      Plant Direct

      Volume: 4 Issue: 5 Pages: 1-14

    • DOI

      10.1002/pld3.219

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Linoleic acid, α-linolenic acid, and monolinolenins as antibacterial substances in the heat-processed soybean fermented with Rhizopus oligosporus2020

    • Author(s)
      Kusumah Dewi、Wakui Misaki、Murakami Mai、Xie Xiaonan、Yukihito Kabuyama、Maeda Isamu
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 84 Issue: 6 Pages: 1285-1290

    • DOI

      10.1080/09168451.2020.1731299

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and non-canonical strigolactones in Lotus japonicus2020

    • Author(s)
      Mori Narumi、Sado Aika、Xie Xiaonan、Yoneyama Kaori、Asami Kei、Seto Yoshiya、Nomura Takahito、Yamaguchi Shinjiro、Yoneyama Koichi、Akiyama Kohki
    • Journal Title

      Phytochemistry

      Volume: 174 Pages: 112349-112365

    • DOI

      10.1016/j.phytochem.2020.112349

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A gain-of-function T-DNA insertion mutant of <i>Marchantia polymorpha</i> hyper-accumulates flavonoid riccionidin A2019

    • Author(s)
      Hamashima Noriko、Xie Xiaonan、Hikawa Mio、Suzuki Tomohiro、Kodama Yutaka
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 3 Pages: 201-204

    • DOI

      10.5511/plantbiotechnology.19.0722a

    • NAID

      130007724772

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-09-25
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lotuslactone, a non-canonical strigolactone from Lotus japonicus2019

    • Author(s)
      Xie Xiaonan、Mori Narumi、Yoneyama Kaori、Nomura Takahito、Uchida Kenichi、Yoneyama Koichi、Akiyama Kohki
    • Journal Title

      Phytochemistry

      Volume: 157 Pages: 200

    • DOI

      10.1016/j.phytochem.2018.10.034

    • URL

      https://pure.teikyo.jp/en/publications/25238d06-3a1b-4f5b-b3c8-aa7fe7d99c98

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genetic and Biochemical Diversity for <i>N</i>-acylhomoserine Lactone Biosynthesis in the Plant Pathogen <i>Pectobacterium carotovorum</i> subsp. <i>carotovorum</i>2019

    • Author(s)
      Morohoshi Tomohiro、Ogasawara Yuto、Xie Xiaonan、Hamamoto Hiroshi、Someya Nobutaka
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 4 Pages: 429-435

    • DOI

      10.1264/jsme2.ME19105

    • NAID

      130007776124

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Corrigendum to: Which are the major players, canonical or non-canonical strigolactones?2019

    • Author(s)
      Yoneyama Koichi、Xie Xiaonan、Yoneyama Kaori、Kisugi Takaya、Nomura Takahito、Nakatani Yoshifumi、Akiyama Kohki、McErlean Christopher S P
    • Journal Title

      Journal of Experimental Botany

      Volume: 70 Issue: 6 Pages: 1987-1987

    • DOI

      10.1093/jxb/erz001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Regulation of biosynthesis, perception, and functions of strigolactones for promoting arbuscular mycorrhizal symbiosis and managing root parasitic weeds2019

    • Author(s)
      Yoneyama Koichi、Xie Xiaonan、Yoneyama Kaori、Nomura Takahito、Takahashi Ikuo、Asami Tadao、Mori Narumi、Akiyama Kohki、Kusajima Miyuki、Nakashita Hideo
    • Journal Title

      Pest Management Science

      Volume: 2 Issue: 9 Pages: 200-205

    • DOI

      10.1002/ps.5401

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] OsPIN1b is Involved in Rice Seminal Root Elongation by Regulating Root Apical Meristem Activity in Response to Low Nitrogen and Phosphate2018

    • Author(s)
      Sun Huwei、Tao Jinyuan、Bi Yang、Hou Mengmeng、Lou Jiajing、Chen Xinni、Zhang Xuhong、Luo Le、Xie Xiaonan、Yoneyama Koichi、Zhao Quanzhi、Xu Guohua、Zhang Yali
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 200-205

    • DOI

      10.1038/s41598-018-29784-x

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] N-Acylhomoserine lactone-mediated quorum sensing regulates biofilm structure in Methylobacterium populi P-1M, an isolate from a pink-pigmented household biofilm2018

    • Author(s)
      Morohoshi Tomohiro、Xie Xiaonan、Ikeda Tsukasa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 83 Issue: 1 Pages: 174-180

    • DOI

      10.1080/09168451.2018.1518701

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of S-abscisic acid treatment on fruit color and quality in ‘Kyoho’ grape2018

    • Author(s)
      Yamane K.、Xie X.、Kurokura T.
    • Journal Title

      Acta Horticulturae

      Volume: 2 Issue: 1206 Pages: 239-246

    • DOI

      10.17660/actahortic.2018.1206.33

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Conversion of carlactone to carlactonoic acid is a conserved function of MAX1 homologs in strigolactone biosynthesis.2018

    • Author(s)
      Yoneyama K, Mori N, Sato T, Yoda A, Xie X, Okamoto M, Iwanaga M, Ohnishi T, Nishiwaki H, Asami T, Yokota T, Akiyama K, Yoneyama K, Nomura T.
    • Journal Title

      New Phytol.

      Volume: 218 Issue: 4 Pages: 1522-1533

    • DOI

      10.1111/nph.15055

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イオンモビリティ MS による植物ホルモンの超高感度分析2020

    • Author(s)
      謝肖男
    • Organizer
      日本農芸化学会
    • Related Report
      2019 Research-status Report
  • [Presentation] コムギが産する根寄植物発芽刺激物質の探索2020

    • Author(s)
      竹原千恵、謝肖男
    • Organizer
      農薬学会
    • Related Report
      2019 Research-status Report
  • [Presentation] マメ科植物種子発芽における種皮、子葉、幼芽、胚軸、幼根のアブシジン酸の定量2019

    • Author(s)
      竹原千恵 、岡本昌憲、米山弘一、謝 肖男
    • Organizer
      農薬学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒマワリにおける ε-カーラクトンのヘリオラクトンへの 変換2019

    • Author(s)
      留河愛梨,謝 肖男,米山弘一,秋山康紀
    • Organizer
      農芸化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 食物アレルギー性下痢に対する抑制効果を示すユズ果皮 抽出物成分の解析2019

    • Author(s)
      松岡歩実,謝 肖男,粟野峻史,千野 晶,東 徳洋, 山田 潔
    • Organizer
      農芸化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 日本国内で分離された野菜類病原性 Pseudomonas 属細 菌における Quorum Sensing の多様性解析2019

    • Author(s)
      諸星知広,伊藤雄大,小笠原祐斗,謝 肖男, 染谷信孝
    • Organizer
      農芸化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] マコモタケの共生・不稔に関わる遺伝子および化合物の探索2019

    • Author(s)
      謝 肖男,崔 宰熏,河岸洋和, 鈴木智大
    • Organizer
      農芸化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ミヤコグサの 5DS および lotuslactone 生合成における MAX1 下流経路の解析2019

    • Author(s)
      森 愛美,謝 肖男,米山香織,野村崇人, 米山弘一,秋山康紀
    • Organizer
      農芸化学会
    • Related Report
      2018 Research-status Report
  • [Presentation] イオンモビリティMSによる植物ホルモンの超高感度分析2018

    • Author(s)
      謝肖男・ 野村崇人・ 米山弘一
    • Organizer
      Mass Spectrometry and Proteomics 2018
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] ストリゴラクトン生合成に関与するソルガム LGS1 酵素の機能解析2018

    • Author(s)
      依田彬義、森 愛美、謝 肖男、米山香織、秋山康紀、米山弘一、野村崇人
    • Organizer
      植物化学調節学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ストリゴラクトン生合成酵素 LBO の機能解析2018

    • Author(s)
      米山香織1,2、秋山康紀、高島 岬、依田彬義、謝 肖男、米山弘一、野村崇人
    • Organizer
      植物化学調節学会
    • Related Report
      2018 Research-status Report
  • [Presentation] マメ科植物種子発芽における種皮、子葉、幼芽、胚軸、幼根のアブシジン酸の定量2018

    • Author(s)
      竹原千恵 、岡本昌憲、米山弘一、謝 肖男
    • Organizer
      植物化学調節学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ストリゴラクトン生合成のフィードバック制御2018

    • Author(s)
      米山香織、謝 肖男、米山弘一、野村崇人
    • Organizer
      植物化学調節学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒマワリにおける ε-カーラクトンのヘリオラクトンへの変換2018

    • Author(s)
      留河愛梨、謝 肖男、米山弘一、秋山康紀
    • Organizer
      植物化学調節学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 植物における内生ヒドロキシカーラクトン酸メチルの同定2018

    • Author(s)
      高島 岬、米山香織、謝 肖男、野村崇人、米山弘一、秋山康紀
    • Organizer
      植物化学調節学会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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