| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
ADP ribosylation, one of post-translational modifications, is a reaction that transfers ADP ribose from nicotinamide adenine dinucleotide (NAD+) to proteins and plays an important role in a variety of cellular processes including DNA repair and transcriptional regulation. Its biological importance has widely been investigated but detailed functions of ADP ribosylation remain poorly understood. The main difficulty in the molecular level research is to obtain homogeneous ADP ribosylated molecules. In this study, we develop a highly efficient method for synthesis of ADP ribosyl molecules. We established protecting-free pyrophosphate formation by phosphate cross-coupling reaction and applied it to chemical synthesis of mono- and di-ADP riboses.
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