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Detection of viable but non-culturable foodborne pathogens

Research Project

Project/Area Number 18K05470
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionAzabu University (2021-2023)
Obihiro University of Agriculture and Veterinary Medicine (2018-2020)

Principal Investigator

Kawamoto Keiko  麻布大学, 獣医学部, 教授 (20360977)

Project Period (FY) 2018-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsVBNC / サルモネラ / ストレス応答 / 食品衛生 / 必須遺伝子 / ABCトランスポーター / Quorum Sensing / 細菌 / 食中毒 / 発現解析 / プロテオーム解析 / 病原菌
Outline of Final Research Achievements

A viable but non-culturable" (VBNC) state is recognized as a survival strategy employed by many bacteria in response to adverse environmental conditions. In this state, bacteria maintain low metabolic activity and remain viable, but fail to form colonies on standard growth media despite the presence of sufficient living cells. In this study, we have identified several VBNC-associated proteins by comparative two-dimensional fluorescence gel electrophoresis between the stationary phase and the VBNC state in Salmonella Typhimurium. The deletion of these molecules altered VBNC responses. Our results offer insights into the molecular mechanisms of the VBNC state, contributing to our understanding of this bacterial survival strategy. These findings may lead to new methods for detecting and controlling VBNC bacteria, with potential benefits for food safety and public health.

Academic Significance and Societal Importance of the Research Achievements

VBNC細菌は食中毒の潜在的な原因となるため、そのメカニズムの理解は新たな検出方法や制御手段の開発は、食品の安全性向上に貢献する。さらに、環境保全の観点からも、特に廃水処理などにおいてVBNC細菌の存在が問題となっており、そのメカニズム解明は効果的な環境管理につながる。また、現在の培養技術では検出できないVBNC状態の細菌を研究することで、新しい培養方法や検出技術の開発が進み、これまで見逃されていた細菌の研究が進展する可能性がある。このように、VBNC状態の細菌のメカニズム解明は、公衆衛生、食品科学、環境衛生など多岐にわたる分野に新知見を与え、健康と安全の向上に貢献することが期待できる。

Report

(6 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (7 results)

All 2023 2022

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results) Book (1 results)

  • [Journal Article] Heterogeneous IgE reactivities to <i>Staphylococcus pseudintermedius</i> strains in dogs with atopic dermatitis, and the identification of DM13-domain-containing protein as a bacterial IgE-reactive molecule2022

    • Author(s)
      Takemura-Uchiyama Iyo、Tsurui Hiroki、Shimakura Hidekatsu、Nasukawa Tadahiro、Imanishi Ichiro、Uchiyama Jumpei、Fukuyama Tomoki、Sakamoto Shuji、Morisawa Keiko、Fujimura Masato、Murakami Hironobu、Kanamaru Shuji、Kurokawa Kenji、Kawamoto Keiko、Iyori Keita、Sakaguchi Masahiro
    • Journal Title

      FEMS Microbiology Letters

      Volume: 369 Issue: 1 Pages: 1-7

    • DOI

      10.1093/femsle/fnac019

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of a <sub>D</sub>-Octaarginine-linked polymer as a transfection tool for transient and stable transgene expression in human and murine cell lines2022

    • Author(s)
      Sakuma S, Okamoto M, Matsushita N, Ukawa M, Tomono T, Kawamoto K, Ikeda T, Sakuma S.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 84 Issue: 4 Pages: 484-493

    • DOI

      10.1292/jvms.21-0647

    • NAID

      130008158321

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] マウス肥満細胞腫細胞株へのRNP複合体導入検討ならびに 機能遺伝子欠損細胞の作製2022

    • Author(s)
      磯貝亮弥、保坂真希、佐藤祐介、川本惠子、岡本まり子
    • Organizer
      第165回日本獣医学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 膜透過性ペプチド固定化高分子を用いたマウス樹状細胞のゲノム編集誘導の試み2022

    • Author(s)
      志水孝之、鵜川真実、佐久間信至、佐藤祐介、川本恵子、岡本まり子
    • Organizer
      第165回日本獣医学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 高齢動物におけるT細胞輸注療法の開発を目的とした、加齢によるイヌT細胞性状への影 響2022

    • Author(s)
      山内章寛、吉本翔、工藤綾乃、川本恵子、永根大幹、岡本まり子、山下匡、金井詠一、吉田大実、高木哲
    • Organizer
      第165回日本獣医学会
    • Related Report
      2022 Research-status Report
  • [Presentation] TCRシグナル刺激によるイヌTCRレパートリーへの影響2022

    • Author(s)
      塩川 麗良、岡本 憲明、渡邉 健司2、水上 洋一、永根 大幹、山内 章寛、金井 詠一、高木 哲、山下 匡、佐藤 祐介、川本 恵子、岡本まり子
    • Organizer
      第165回日本獣医学会
    • Related Report
      2022 Research-status Report
  • [Book] 生食のはなしーリスクを知っておいしく食べるー川本 伸一(編集代表)/朝倉 宏・稲津 康弘・畑江 敬子・山﨑 浩司(編)2023

    • Author(s)
      川本恵子「赤痢菌」
    • Total Pages
      160
    • Publisher
      朝倉書店
    • ISBN
      9784254431308
    • Related Report
      2022 Research-status Report

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Published: 2018-04-23   Modified: 2025-01-30  

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