• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Detection of foodborne pathogens by using volatile organic compounds released from them as indices

Research Project

Project/Area Number 18K05477
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionFukuoka Women's University

Principal Investigator

KOBAYASHI HIROSHI  福岡女子大学, 国際文理学部, 准教授 (00610255)

Co-Investigator(Kenkyū-buntansha) 石川 洋哉  福岡女子大学, 国際文理学部, 教授 (00325490)
Project Period (FY) 2018-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords食中毒細菌 / MVCs / 簡易迅速検査 / 迅速検査 / 超高速GC / 細菌由来揮発性化合物 / 細菌検査 / 揮発性化合物 / 食品危害細菌 / 微生物由来揮発性化合物
Outline of Final Research Achievements

A simple and rapid screening method for foodborne bacteria using volatile compounds (odor) produced by bacteria as an indicator was established. The analysis of microbial volatile compounds (MVCs) was performed using an electronic olfactory system. Under optimized conditions, some bacteria could be identified as genera or species in pure systems. The system was also applicable for the detection of foodborne bacteria (Staphylococcus aureus, Escherichia coli, or pathogenic Escherichia coli) in milk and ready-to-eat vegetables.

Academic Significance and Societal Importance of the Research Achievements

食品の細菌検査は、培養法、イムノクロマト法、PCR法など、増菌培養液から菌体(核酸、タンパク質)を取り出して検出する方法が多く用いられている。これらは確実・高感度である一方、培養液を取り出す際の液の飛び散りなど、食品工場においては汚染リスクになる問題があった。本研究で確立した検出法は、測定対象が培養液中の菌体ではなく、菌体が産生した揮発性化合物であるため、培養液を取り出すリスクはない。また、固相マイクロ抽出 (SPME) 法を用いることで、揮発性成分の濃縮が簡便になるため、簡易・迅速な食中毒細菌の検出が可能になった。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (1 results)

All 2019

All Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] Development of Bacteria Screening Method by Using Microbial Volatile Compounds2019

    • Author(s)
      小林 弘司、阿部 萌瑛、太田 香穂、南 育子、石川 洋哉
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2018-04-23   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi