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Development of the methods for evaluating the ability of absorption in human jejunum and for assessing the absorptive substance-mediated changes in gut immunity

Research Project

Project/Area Number 18K05503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionShinshu University

Principal Investigator

Maejima Daisuke  信州大学, 医学部, 特任講師 (20715130)

Co-Investigator(Kenkyū-buntansha) 河合 佳子  信州大学, 医学部, 特任教授 (10362112)
林 もゆる  信州大学, 医学部, 特任助教 (60548147)
大橋 俊夫  信州大学, 医学部, 特任教授 (80020832)
Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords水分摂取 / 養生訓 / podoplanin / IL-22 / 自然免疫 / enterochromaffin 細胞 / セロトニン / 腸リンパ / 長鎖脂肪酸 / 血漿アルブミン / ヒト臨床研究 / 腸管免疫 / 機能性食品 / ILC-3
Outline of Final Research Achievements

We investigated effects of water intake on podoplanin and IL-22 expressions in rat jejunal villi. Water intake accelerated podoplanin and Il-22 levels. ATP increased IL-22 mRNA expression in ILC-3. Hence, water intake may regulate the mucosal immunity. We investigated the roles of water intake in serotonin production ad release in rat jejunum. Water intake increased serotonin concentration in the portal vein, but not in the mesenteric lymph vessel. The flux of serotonin through the portal vein was significantly larger than that through the mesenteric lymph vessel. Water intake decreased the density of granules of serotonin in the enterochromaffin cells in the jejunal villi. The intravenous administration of serotonin increased significantly mesenteric lymph flow and the concentrations of albumin and IL-22. Serotonin released from enterochromaffin cells by water intake was mainly transported through the portal vein, which increased mesenteric lymph formation.

Academic Significance and Societal Importance of the Research Achievements

コロナの問題でヒトの臨床研究から動物実験に研究手法を変更した。江戸時代の養生訓で言われてきた「水分摂取」の健康法についてその科学的根拠を明確にするために本研究を実施した。水分を摂取したラットは空腸粘膜下組織に壁内の線維芽細胞からATPの分泌を促し、ポドプラニンを組織周囲に発現し、腸リンパへの水分吸収とそのリンパ液への自然免疫を担うIL-22輸送を誘導する事を発見した。併せて水分摂取は空腸壁のenterochromaffin細胞からセロトニン分泌亢進とセロトニンによるIL-22の遺伝子発現を増強する事を確認した。以上、水分摂取の健康維持の科学的根拠を明確にした点が本研究の社会的意義である。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (14 results)

All 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity2021

    • Author(s)
      Hayashi Moyuru、Watanabe-Asaka Tomomi、Nagashio Sachiho、Kaidoh Maki、Yokoyama Yumiko、Maejima Daisuke、Kajihara Ryo、Amari Kei、Arai Nariaki、Kawai Yoshiko、Ohhashi Toshio
    • Journal Title

      American Journal of Physiology-Gastrointestinal and Liver Physiology

      Volume: 320 Issue: 1 Pages: G54-G65

    • DOI

      10.1152/ajpgi.00303.2020

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluating Lymph Flow through the Thoracic Duct using Urine Osmolarity in Human Participants.2020

    • Author(s)
      Moyuru Hayashi, Tomomi Watanabe-Asaka, Yoshiko Kawai, Toshio Ohhashi et al
    • Journal Title

      Lymphat Res Biol

      Volume: in press Issue: 4 Pages: 351-356

    • DOI

      10.1089/lrb.2019.0054

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: New concept of absorption in jejunum.2019

    • Author(s)
      Nagashio S, Ajima K, Maejima D, Sanjo H, Kajihara R, Hayashi M, Watanabe-Asaka T, Kaidoh M, Yokoyama Y, Taki S, Kawai Y, Ohhashi T
    • Journal Title

      Am J Physiol Gastrointestinal and Liver Physiology

      Volume: 316

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: New concept of absorption in jejunum.2019

    • Author(s)
      Nagashio S, Ajima K, Maejima D, Sanjo H, Kajihara R, Hayashi M, Watanabe-Asaka T, Kaidoh M, Yokoyama Y, Taki S, Kawai Y, Ohhashi T
    • Journal Title

      Am J Physiol Gastrointestinal and Liver Physiology

      Volume: 316

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 飲水により空腸より分泌されたATPがIL-22依存性の自然免疫を調節する2020

    • Author(s)
      林もゆる、浅香智美、前島大輔、河合佳子、大橋俊夫
    • Organizer
      第44回日本リンパ学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 流れ刺激がリンパ管の機能におよぼす影響について2020

    • Author(s)
      河合佳子、浅香智美、林もゆる
    • Organizer
      第61回日本脈管学会総会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Water intake accelerates mesenteric lymph flow and the total flux of long-chain fatty acids in rats.2019

    • Author(s)
      Ohhashi T, Kawai Y
    • Organizer
      Lymphatic Forum 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Proposed new lymphology combined with lymphatic physiology, innate immunology, and oncology: From lymph flow points of view2019

    • Author(s)
      Kawai Y, Hayashi M, Watanabe-Asaka T, Ohhashi T
    • Organizer
      Lymphatic Forum 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Physiological evidence that mesenteric lymph has been called as white blood2019

    • Author(s)
      Tomomi Watanabe-Asaka, Daisuke Maejima, Moyuru Hayashi, Yoshiko Kawai, Toshio Ohhashi
    • Organizer
      FAOPS2019
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒト胸管リンパ流量を尿浸透圧変化から推定する測定法の開発:リンパ浮腫療法士の実技試験への応用2019

    • Author(s)
      林もゆる
    • Organizer
      第43回日本リンパ学会総会
    • Related Report
      2018 Research-status Report
  • [Book] Lymph Node Metastasis in Gastrointestinal Cancer2019

    • Author(s)
      Natsugoe S ed
    • Total Pages
      358
    • Publisher
      Springer Nature
    • Related Report
      2019 Research-status Report 2018 Research-status Report
  • [Patent(Industrial Property Rights)] ヒトの胸管リンパ液の流れの評価方法および評価具2019

    • Inventor(s)
      大橋俊夫、河合佳子
    • Industrial Property Rights Holder
      大橋俊夫、河合佳子
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] リンパ液流動性向上剤およびリンパ液流動性向上方法2019

    • Inventor(s)
      大橋俊夫
    • Industrial Property Rights Holder
      大橋俊夫
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] リンパ液流動性向上のための飲食品2019

    • Inventor(s)
      大橋俊夫
    • Industrial Property Rights Holder
      大橋俊夫
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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