| Project/Area Number |
18K05505
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Kobe University |
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Principal Investigator |
Fukuda Itsuko 神戸大学, 農学研究科, 助教 (50418943)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 腸内細菌叢 / ファイトケミカル / 腸内環境 / ケルセチン / イヌリン / ヒト腸内細菌叢 / 腸内動態 / 腸内フローラ |
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| Outline of Final Research Achievements |
In this study, I focused on quercetin (flavonoids), inulin (polysaccharides), lutein (carotenoids), and glucoraphanine (sulfur compounds) among phytochemicals, and evaluated their effects on intestinal dynamics and intestinal environment by using Kobe University Human Intestinal Microbiota Model (KUHIMM) and human colon adenocarcinoma HT29-MTX cells. While quercetin showed antibacterial activity, inulin was confirmed to act as a prebiotic, and inulin was found to be decomposed by the intestinal microbiota. Lutein and glucoraphanin did not affect the intestinal microbiota and its metabolites under the conditions of this study. Also, intestinal microbiota metabolites did not affect HT29-MTX cells.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、個々人で異なる腸内エコシステムにおいて、ファイトケミカルがどのような腸内動態を辿り、腸内環境にどのような影響をおよぼすのかを培養系ヒト腸内細菌叢モデルとヒト結腸がん由来HT29-MTX細胞を用いて解明した。腸内エコシステムは個々人で異なるため、個々人の糞便細菌叢を培養スターターとした培養系ヒト腸内細菌叢モデルを使用することに独自性があり、ヒト介入試験や動物実験では入手できない試料採取と解析を可能にできる点で学術的意義が高い。
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