Screening and production of novel trichothecenes to establish survey system of mycotoxins in food
| Project/Area Number |
18K05524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Toyo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
木村 真 名古屋大学, 生命農学研究科, 准教授 (20261167)
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| Project Period (FY) |
2018-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 新規トリコテセン / Fusarium / 遺伝子組換え株 / d-typeトリコテセン / MS/MSライブラリー / 糖抱合 / カビ毒 / トリコテセン / 食の安全 / LC-MS/MS / マイコトキシン / データベース化 |
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| Outline of Final Research Achievements |
Trichothecenes are a group of mycotoxins that contain trichothecene skeleton in common. They are produced when filamentous fungi such as Fusarium spp. infect wheat and other important crops. Recently, a series of studies reported previously unknown trichothecenes such as newly evolved trichothecenes and conjugated trichothecenes. These novel trichothecenes might not be able to be detected by conventional methods, thus, can pose a major threat to food safety. Therefore, in this study, we aimed to create an MS/MS library of various trichothecenes including novel ones, so that we can detect and identify newly evolved trichothecenes emerged in the field. In this study, we created and identified several analogs of T-2 toxin, which is highly toxic and frequently found in agricultural products in Japan. We also succeeded to produce and identify novel glucose conjugates of trichothecenes. These results are expected to contribute to build a novel food safety inspection system.
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| Academic Significance and Societal Importance of the Research Achievements |
トリコテセン類は、農作物を汚染するカビ毒として食の安全を脅かす存在である。しかしこれまで、トリコテセンは十分な防除がなされてきたとは言えない状況であった。近年、新たな進化型トリコテセンや抱合型トリコテセンの存在が次々と明らかになってきており、その生合成経路や進化を解明することは学術的意義が高いと言える。また、これらの新興トリコテセンの同定システムの構築は、トリコテセン防除において極めて高い社会的意義を持つ。本研究では、複数の新規トリコテセンを生産、同定することができ、それらをMS/MSライブラリーに組み込み、その毒性の検証にも成功した。今後、さらにこれらのリスクを検証することが重要となろう。
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Report
(2 results)
Research Products
(17 results)
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[Journal Article] Comparison of HPLC-UV and LC-MS methods for evaluating the amount of deoxynivalenol-type trichothecenes in axenic solid culture of <i>Fusarium graminearum</i>2019
Author(s)
１． TANAKA, A., SHINKAI, K., MAEDA, K., NAKAJIMA, Y., ISHII, S., YOSHIDA, Y., KIMURA, M. and TAKAHASHI-ANDO, N.
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Journal Title
JSM Mycotoxins
Volume: 69
Issue: 1
Pages: 15-17
DOI
NAID
ISSN
0285-1466, 1881-0128
Year and Date
2019-01-31
Related Report
Peer Reviewed / Open Access
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[Journal Article] Exploring an artificial metabolic route in Fusarium sporotrichioides: Production and characterization of 7-Hydroxy T-2 toxin.2018
Author(s)
KAMATA, K., SATO, H., MAEDA, K., FURIHATA, K., AIKAWA, S., ADACHI, K., TANAKA, A., TOKAI, T., NAKAJIMA, Y., YOSHIDA, Y., SAKUDA, S., KIMURA, M. and TAKAHASHI-ANDO, N.
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Journal Title
J. Nat. Prod.
Volume: 印刷中
Issue: 4
Pages: 1041-1044
DOI
Related Report
Peer Reviewed / Open Access
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