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In situ visualization of intestinal absorption processes of polyphenols by using MALDI-MS imaging

Research Project

Project/Area Number 18K05534
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionKyushu University

Principal Investigator

Tanaka Mitsuru  九州大学, 五感応用デバイス研究開発センター, 准教授 (70584209)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsMS イメージング / 腸管吸収 / ポリフェノール / 代謝 / MALDI-MS / イメージング / 腸管 / 吸収 / MALDI-MS imaging / 可視化
Outline of Final Research Achievements

In the present study, MALDI-MS imaging was used as a visualization technique to investigate the intestinal absorption of polyphenols. Nifedipine/phytic acid-aided MALDI-MS imaging was performed to visualize theaflavin-3’-O-gallate (TF3’G) and epicatechin-3-O-gallate (ECG) in the rat jejunum. Non-absorbable TF3’G was successfully visualized at the apical region, whereas absorbable ECG was detected throughout the rat jejunum. MALDI-MS imaging was also performed to determine the transport routes of the target metabolites. The inhibitor aided-intestinal MALDI-MS imaging visualized the behavior in which TF3’G and ECG were incorporated into intestinal tissues, followed by efflux back to the apical compartment. In addition, MALDI-MS imaging analyses suggested that TF3’G was resistant to phase II metabolism during the influx/efflux processes, whereas ECG was susceptible to methylation and sulphation reactions.

Academic Significance and Societal Importance of the Research Achievements

これまでの食品成分の吸収性評価は、摂取した成分の血中移行性の評価に終始しているものがほとんどで、実際の吸収を制御する腸管での動態や代謝物を考慮した解析にはほとんど至れていない。対して、本研究にて構築したラット腸管 in situ MALDI-MS イメージング法は、腸管組織での成分の透過過程、並びに、一連の代謝物を可視化することが可能な方法である。すなわち、本法はこれまではブラックボックスとされてきた腸管での吸収動態を可視化という直截的な手法で評価可能であり、生体利用性の解明を基盤とした食機能研究の遂行に極めて有用な評価法であると考えられる。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Matrix-assisted laser desorption/ionization mass spectrometry-guided visualization analysis of intestinal absorption of acylated anthocyanins in Sprague-Dawley rats2021

    • Author(s)
      Hahm Tae Hun、Tanaka Mitsuru、Nguyen Huu-Nghi、Tsutsumi Ayaka、Aizawa Koichi、Matsui Toshiro
    • Journal Title

      Food Chemistry

      Volume: 334 Pages: 127586-127586

    • DOI

      10.1016/j.foodchem.2020.127586

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] MALDI-MSイメージング法による食品成分の腸管吸収過程の可視化2020

    • Author(s)
      咸 兌勳, 松井利郎, 田中 充
    • Journal Title

      食品加工技術

      Volume: 39 Pages: 12-20

    • NAID

      40022167881

    • Related Report
      2019 Research-status Report
  • [Journal Article] Detection and Visualization of Food-derived Polyphenols by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Imaging2019

    • Author(s)
      Huu-Nghi Nguyen, Tae Hun Ham, Toshiro Matsui, and Mitsuru Tanaka
    • Journal Title

      Sensors and Materials

      Volume: 31 Issue: 7 Pages: 2333

    • DOI

      10.18494/SAM.2019.2371

    • ISSN
      0914-4935, 2435-0869
    • Year and Date
      2019-07-30
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel in situ visualisation of rat intestinal absorption of polyphenols via matrix-assisted laser desorption/ionisation mass spectrometry imaging2019

    • Author(s)
      Nguyen Huu-Nghi、Tanaka Mitsuru、Li Baorui、Ueno Tomoya、Matsuda Hideki、Matsui Toshiro
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s41598-019-39405-w

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Brain-transportable dipeptides across the blood-brain barrier in mice2019

    • Author(s)
      Tanaka Mitsuru、Dohgu Shinya、Komabayashi Genki、Kiyohara Hayato、Takata Fuyuko、Kataoka Yasufumi、Nirasawa Takashi、Maebuchi Motohiro、Matsui Toshiro
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-019-42099-9

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] MALDI-MS Imaging法を用いたアシル化アントシアニン類の腸管吸収挙動の解明2020

    • Author(s)
      咸 兌勳,Huu Nghi Nguyen,田中 充, 堤 彩香,相澤 宏, Qing Qiang Hu, 松井利郎
    • Organizer
      第74回日本栄養食糧学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Importance of the ADME Analysis for Peptide Functionality2019

    • Author(s)
      Mitsuru Tanaka, Toshiro Matsui
    • Organizer
      Frontiers in Chemistry, Biology, and Earth Science
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of absorption and metabolism of polyphenols in rat intestine by nifedipine aided-MALDI-MS imaging2019

    • Author(s)
      Mitsuru Tanaka, Tae Hun Ham, Huu-Nghi Nguyen, Toshiro Matsui
    • Organizer
      The 7th International Conference on Food Factors
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 腸管を作用場とする機能性ポリフェノールの吸収・代謝挙動の可視化2019

    • Author(s)
      田中 充、グエン フナイ、李宝瑞、松井 利郎
    • Organizer
      日本農芸化学会 2019 大会
    • Related Report
      2018 Research-status Report
  • [Presentation] MALDI-MS Imaging法を用いた非吸収性ポリフェノールの腸管分布解析2018

    • Author(s)
      咸 兌勳,Huu-Nghi Nguyen,田中 充,Baorui Li, 山本采佳, 松田秀喜, 松井利郎
    • Organizer
      平成30年度 日本食品科学工学会西日本支部大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Analytical methods for evaluation of food compounds2018

    • Author(s)
      Mitsuru Tanaka
    • Organizer
      The Third International Five-Sense Symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] MALDI-MS imaging法を用いた ポリフェノール類の腸管吸収挙動の可視化2018

    • Author(s)
      清原隼人,Huu-Nghi Nguyen,田中 充,Baorui Li, 山本采佳, 松田秀喜, 松井利郎
    • Organizer
      日本食品科学工学会 第65回大会
    • Related Report
      2018 Research-status Report

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Published: 2018-04-23   Modified: 2022-01-27  

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