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Environmental adaptation in response to extracellular signals in fungal growth

Research Project

Project/Area Number 18K05545
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

TAKESHITA Norio  筑波大学, 生命環境系, 准教授 (20745038)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords糸状菌 / 菌糸 / カルシウム / アクチン / カルモジュリン / エキソサイトーシス / 屈性 / 環境適応 / マイクロ流体デバイス / 極性
Outline of Final Research Achievements

Filamentous fungi are composed of tubular cells called mycelium, which grow by extending their tips. The apical growth of mycelium is caused by the cyclic influx of Ca2+, which synchronizes actin polymerization and enzyme secretion to gradually elongate the mycelium. In this study, we visualized the intracellular Ca2+ conduction by live imaging and analyzed its characteristics. In addition, to investigate how Ca2+ signals are transmitted downstream, we constructed strains expressing fusion proteins of Calmodulin CaM or Calmodulin-dependent kinase, CmkA, CmkB, and CmkC, to which GFP was added. We purified the interacting proteins by immunoprecipitation with GFP antibody. They were subjected to LC-MS/MS mass spectrometry to identify 20-60 candidate targets for CaM, CmkA, CmkB, and CmkC, respectively. Interestingly, there was little overlap among them, suggesting that each protein uses different signaling strategies by targeting different proteins.

Academic Significance and Societal Importance of the Research Achievements

本研究では、細胞内のCa2+の伝導をライブイメージングで可視化しその特性を解析した。さらに、Ca2+のシグナルがどのように下流に伝わるかを調べるため、CalmodulinまたはCalmodulin-dependent kinaseのターゲット候補をそれぞれ20-60個同定した。それらの重複はほとんど見られず、それぞれが異なるタンパク質をターゲットとすることで、シグナル伝達を使い分けていることが示唆された。本研究は、カビが関わる醸造・発酵、動植物への病原機構の解明、カビの高い環境適応能の理解と人間生活におけるカビ対策に貢献する。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (10 results)

All 2021 2020 2019 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (4 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] Trade-off between Plasticity and Velocity in Mycelial Growth2021

    • Author(s)
      Sayumi Fukuda, Riho Yamamoto, Naoki Yanagisawa, Naoki Takaya, Yoshikatsu Sato, Meritxell Riquelme, Norio Takeshita
    • Journal Title

      mBio

      Volume: 12 Issue: 2 Pages: 1-32

    • DOI

      10.1128/mbio.03196-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Invasive growth of Aspergillus oryzae in rice koji and increase of nuclear number2020

    • Author(s)
      Yasui Mizuki、Oda Ken、Masuo Shunsuke、Hosoda Shuji、Katayama Takuya、Maruyama Jun-ichi、Takaya Naoki、Takeshita Norio
    • Journal Title

      Fungal Biology and Biotechnology

      Volume: 7 Issue: 1 Pages: 8-8

    • DOI

      10.1186/s40694-020-00099-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fungal mycelia and bacterial thiamine establish a mutualistic growth mechanism.2020

    • Author(s)
      Abeysinghe G, Kuchira M, Kudo G, Masuo S, Ninomiya A, Takahashi K, Utada AS, Hagiwara D, Nomura N, Takaya N, Obana N, Takeshita N.
    • Journal Title

      Life Sci Alliance

      Volume: 3 Issue: 12 Pages: e202000878-e202000878

    • DOI

      10.26508/lsa.202000878

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fungal research in Japan: tradition and future2020

    • Author(s)
      Takeshita Norio
    • Journal Title

      Fungal Biology and Biotechnology

      Volume: 7 Issue: 1 Pages: 14-14

    • DOI

      10.1186/s40694-020-00104-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Control of Actin and Calcium for Chitin Synthase Delivery to the Hyphal Tip of Aspergillus2019

    • Author(s)
      Takeshita Norio
    • Journal Title

      Curr Top Microbiol Immunol

      Volume: 2020 Pages: 1-17

    • DOI

      10.1007/82_2019_193

    • ISBN
      9783030499273, 9783030499280
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 糸状菌と細菌の相利共生2021

    • Author(s)
      竹下典男
    • Organizer
      農芸化学会シンポジウム「微生物研究の新機軸としての相互作用研究」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 真菌と細菌の相利共生2021

    • Author(s)
      竹下典男
    • Organizer
      日本細菌学会「集団微生物学と細菌バイオフィルム研究の前線」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 菌糸の高速道路と細菌の通行料2020

    • Author(s)
      竹下典男
    • Organizer
      新学術領域「ポストコッホ生態」公開シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 糸状菌の先端生長におけるカルシウム情報伝達経路2019

    • Author(s)
      芹澤 知子, 桝尾 俊介, 別役 重之, 高谷 直樹, 竹下 典男
    • Organizer
      糸状菌分子生物学コンファレンス
    • Related Report
      2019 Research-status Report
  • [Remarks]

    • URL

      https://fungalcell.com

    • Related Report
      2020 Annual Research Report

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Published: 2018-04-23   Modified: 2022-01-27  

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