| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Filamentous fungi are composed of tubular cells called mycelium, which grow by extending their tips. The apical growth of mycelium is caused by the cyclic influx of Ca2+, which synchronizes actin polymerization and enzyme secretion to gradually elongate the mycelium. In this study, we visualized the intracellular Ca2+ conduction by live imaging and analyzed its characteristics. In addition, to investigate how Ca2+ signals are transmitted downstream, we constructed strains expressing fusion proteins of Calmodulin CaM or Calmodulin-dependent kinase, CmkA, CmkB, and CmkC, to which GFP was added. We purified the interacting proteins by immunoprecipitation with GFP antibody. They were subjected to LC-MS/MS mass spectrometry to identify 20-60 candidate targets for CaM, CmkA, CmkB, and CmkC, respectively. Interestingly, there was little overlap among them, suggesting that each protein uses different signaling strategies by targeting different proteins.
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