| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We have studied on the molecular mechanisms of storage of postmeiotic mRNAs and their deadenylation, which accompanies translational activation, during murine spermiogenesis. It was found that translationally repressed mRNAs are associated with cap-binding protein eIF4E and poly(A)-binding protein PABPC1. Y-box RNA-binding protein 2 (YBX2), which has been thought to be involved in global translational repression, may contribute to stabilization and storage of postmeiotic mRNAs. We also revealed that formation of ladder-like deadenylation intermediates with a periodic spacing of ~30 nt is mediated by PABPC and canonical deadenylase complexes and is intrinsic to neither postmeiotic mRNAs nor late haploid cells.
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