| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we revealed the metabolic properties of N-acetylglucosamine (GlcNAc), a component monosaccharide of chitin, in the GlcNAc-assimilating yeast Scheffersomyces stipitis by dynamic metabolic profiling using stable nitrogen isotope (15N)-labeled GlcNAc. We also established a genetic modification techniques for this yeast. The metabolically-engineered S. stipitis strain produced a valuable aromatic compound resveratrol from various sugars including GlcNAc. Furthermore, we added the chitin degradation ability to this resveratrol-producing strain by using cell-surface engineering technology. The constructed S. stipitis strain successfully produced resveratrol directly from chitin by simultaneous saccharification and fermentation.
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