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Critical roles of autophagy and enzymatic ROS production in the regulation of tapetal programmed cell death in rice

Research Project

Project/Area Number 18K05562
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionSuwa University of Science

Principal Investigator

KURUSU Takamitsu  公立諏訪東京理科大学, 工学部, 准教授 (50422499)

Project Period (FY) 2018-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsオートファジー / 活性酸素種(ROS) / タペート層 / プログラム細胞死 / 花粉発達 / イネ / 転写ネットワーク / 葯分化 / タペート細胞
Outline of Final Research Achievements

We comprehensively analyzed the effect of autophagy disruption on gene expression profile during the tapetal PCD in rice anther development using high-throughput RNA sequencing. Expression of thousands of genes, including specific transcription factors and several proteases required for tapetal degradation, fluctuated synchronously at specific stages during tapetal PCD progression in the wild type anthers, while this fluctuation showed significant delay in the autophagy-deficient mutant Osatg7-1. The state of ROS accumulation was apparently changed and suppressed in the ROS mutant during anther development. The numbers of autophagosome-like structures were reduced in the tapetum of ROS mutant at stage 10 compared to that of control plants. These results suggest that redox signals contribute to anther development as well as promoting pollen maturation, and may be involved in the regulation of tapetal autophagy in rice.

Academic Significance and Societal Importance of the Research Achievements

本研究では、葯発達や花粉への栄養供給細胞であるタペート細胞のPCD過程において、オートファジー誘導やレドックスシグナルの重要性に着目し、遺伝子発現・ROS蓄積を介した時空間的オートファジー制御機構の解明を進め、PCD進行過程におけるROSシグナルとオートファジー機構の生理的意義・重要性を明らかにすることが出来た。本成果は,栽培イネの花粉発達の新たな仕組みの解明だけでなく,将来的にオートファジー制御による作物の細胞内有害物質の低減化への活用が期待される。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (12 results)

All 2021 2020 2019 2018 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] ヘルシンキ大学(フィンランド)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Cell Cycle-Dependence of Autophagic Activity and Inhibition of Autophagosome Formation at M Phase in Tobacco BY-2 Cells2020

    • Author(s)
      Hanamata Shigeru、Kurusu Takamitsu、Kuchitsu Kazuyuki
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 23 Pages: 9166-9166

    • DOI

      10.3390/ijms21239166

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impact of Autophagy on Gene Expression and Tapetal Programmed Cell Death During Pollen Development in Rice2020

    • Author(s)
      Hanamata Shigeru、Sawada Jumpei、Ono Seijiro、Ogawa Kazunori、Fukunaga Togo、Nonomura Ken-Ichi、Kimura Seisuke、Kurusu Takamitsu、Kuchitsu Kazuyuki
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 172-172

    • DOI

      10.3389/fpls.2020.00172

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monitoring autophagy in rice tapetal cells during pollen maturation2019

    • Author(s)
      Hanamata S., Sawada J., Toh B., Ono S., Ogawa K., Fukunaga T., Nonomura KI., Kurusu T., Kuchitsu K.
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 2 Pages: 99-105

    • DOI

      10.5511/plantbiotechnology.19.0417a

    • NAID

      130007672670

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-06-25
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation2019

    • Author(s)
      Sera Yuri、Hanamata Shigeru、Sakamoto Shingo、Ono Seijiro、Kaneko Kentaro、Mitsui Yuudai、Koyano Tomoko、Fujita Naoko、Sasou Ai、Masumura Takehiro、Saji Hikaru、Nonomura Ken-Ichi、Mitsuda Nobutaka、Mitsui Toshiaki、Kurusu Takamitsu、Kuchitsu Kazuyuki
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 18544-18544

    • DOI

      10.1038/s41598-019-54361-1

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] イネの生殖過程におけるオートファジーの重要性2018

    • Author(s)
      来須 孝光、花俣 繁、瀬良 ゆり、朽津 和幸
    • Journal Title

      BSJ-Review

      Volume: 9 Issue: A Pages: 29-35

    • DOI

      10.24480/bsj-review.9a5.00131

    • ISSN
      2432-9819
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] イネの花粉成熟・タペート細胞のプログラム細胞死におけるオートファジー・ROS生成酵素 Rbohの役割2021

    • Author(s)
      小川和准、澤田隼平、福永任吾、花俣繁、橋本研志、小野聖二郎、野々村賢一、木村成介、来須孝光、朽津和幸
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネの花粉成熟・種子登熟における オートファジーの役割とその制御機構2020

    • Author(s)
      小川和准、福永任吾、澤田隼平、瀬良ゆり、花俣繁、小野聖二郎、野々村賢一、木村成介、橋本 研志、来須孝光、朽津和幸
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ葯タペート層のプログラム細胞死・花粉成熟におけるROS生成・オートファジーの役割2020

    • Author(s)
      来須孝光、花俣繁、澤田隼平、福永任吾、小川和准、小野聖二郎、賀屋秀隆、土岐精一、野々村賢一、朽津和幸
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] イネ葯タペート層のプログラム細胞死・花粉成熟におけるROS生成・オートファジーの役割2018

    • Author(s)
      来須孝光、澤田隼平、福永任吾、花俣繁、小野聖二郎、賀屋秀隆、土岐精一、野々村賢一、朽津和幸
    • Organizer
      第36回日本植物細胞分子生物学会(金沢)大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネにおけるオートファジーと活性酸素シグナルネットワークの生理機能の解明2018

    • Author(s)
      来須孝光
    • Organizer
      第36回日本植物細胞分子生物学会(金沢)大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Critical roles of autophagy and ROS in the regulation of tapetal programmed cell death in rice2018

    • Author(s)
      Kurusu T., Sawada J., Fukunaga T., Hanamata S., Ono S., Ogawa K., Kaya H., Toki S., Nonomura K.I, Kuchitsu K.
    • Organizer
      Japan-Finland seminar 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-04-23   Modified: 2022-01-27  

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