Elucidation of circRNA transport pathway
| Project/Area Number |
18K05563
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38060:Applied molecular and cellular biology-related
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| Research Institution | Setsunan University (2020-2021)
Fujita Health University (2018-2019) |
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Principal Investigator |
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| Project Period (FY) |
2018-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2021: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | RNA / 環状RNA / circRNA / スプライシング / 輸送 / 核外輸送 / RNAプロセシング |
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| Outline of Final Research Achievements |
ASO inhibition experiments using human neuroblastoma (SH-SY5Y) cells expressing ciRS-7 have revealed the presence of a complementary sequence in the ciRS-7 intron that is required for cyclization. Bioinformatics analysis and reporter experiments revealed that a repeat sequence called MIR (Mammalian Interspersed Repeats) (SINE) is required for circularization. Furthermore, we discovered many circRNAs produced in an MIR-dependent manner by analyzing RNA-Seq data, and established that MIR-dependent circularization that is highly conserved from mouse to human.
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| Academic Significance and Societal Importance of the Research Achievements |
circRNAのほとんどは細胞質に局在し、主な機能としては①マイクロRNA(miRNA)を吸着することによりmiRNAの転写後発現調節を制御するもの、②RNA結合タンパク質(RBP)の機能を制御するもの、そして③mRNA由来の翻訳枠(ORF)を持ち翻訳を受けるものが存在する(図1)。したがってcircRNAはマイクロRNA(miRNA)や長鎖ノンコーディングRNA(lncRNA)に並ぶ新たな機能性RNA群であり、circRNAの機能解明は、重要な疾患の治療に貢献できる。
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Report
(5 results)
Research Products
(13 results)
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[Journal Article] Spliceostatin A interaction with SF3B1 limits U1 snRNP availability and causes premature cleavage and polyadenylation.2021
Author(s)
Yoshimoto, R., Chhipi-Shrestha, J.K., Schneider-Poetsch, T., Furuno, M., Burroughs, A.M., Noma, S., Suzuki, H., Hayashizaki, Y., Mayeda, A., Nakagawa, S., Kaida, D., Iwasaki, S., Yoshida, M.
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Journal Title
Cell Chem. Biol.
Volume: -
Issue: 9
Pages: 1356-1365
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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